Successful Treatment of a Recalcitrant Staphylococcus epidermidis Prosthetic Knee Infection with Intraoperative Bacteriophage Therapy
Abstract
:1. Introduction
2. Case
3. Methods
3.1. Bacterial Isolation
3.2. Bacteriophage Screening
3.3. Phage Amplification, Purification and Quality Control Testing
3.4. DAIR Procedure and Bacteriophage Administration
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Antibiotic | MIC µg/mL | Interpretation |
---|---|---|
Cefazolin | NA | Resistant |
Ciprofloxacin | ˃2 | Resistant |
Daptomycin | ≤0.25 | Sensitive |
Erythromycin | ˃4 | Resistant |
Gentamicin | ≤1 | Sensitive |
Linezolid | 1 | Sensitive |
Oxacillin | ˃2 | Resistant |
Rifampin | ≤1 | Sensitive |
Tetracycline | ≤2 | Sensitive |
TMS | ˃2/38 | Resistant |
Vancomycin | 1 | Sensitive |
Phage ID | Titer (PFU/mL) | Endotoxin (EU/dose) | USP <71> Sterility Testing |
---|---|---|---|
PM448 | 1 × 1010 | <1 | PASSED |
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Doub, J.B.; Ng, V.Y.; Wilson, E.; Corsini, L.; Chan, B.K. Successful Treatment of a Recalcitrant Staphylococcus epidermidis Prosthetic Knee Infection with Intraoperative Bacteriophage Therapy. Pharmaceuticals 2021, 14, 231. https://doi.org/10.3390/ph14030231
Doub JB, Ng VY, Wilson E, Corsini L, Chan BK. Successful Treatment of a Recalcitrant Staphylococcus epidermidis Prosthetic Knee Infection with Intraoperative Bacteriophage Therapy. Pharmaceuticals. 2021; 14(3):231. https://doi.org/10.3390/ph14030231
Chicago/Turabian StyleDoub, James B., Vincent Y. Ng, Eleanor Wilson, Lorenzo Corsini, and Benjamin K. Chan. 2021. "Successful Treatment of a Recalcitrant Staphylococcus epidermidis Prosthetic Knee Infection with Intraoperative Bacteriophage Therapy" Pharmaceuticals 14, no. 3: 231. https://doi.org/10.3390/ph14030231