Next Article in Journal
Relationship Between Remotely-sensed Vegetation Indices, Canopy Attributes and Plant Physiological Processes: What Vegetation Indices Can and Cannot Tell Us About the Landscape
Next Article in Special Issue
An Electrochemical Detection of Metallothioneins at the Zeptomole Level in Nanolitre Volumes
Previous Article in Journal
Mangrove Forest Distributions and Dynamics in Madagascar (1975–2005)
Previous Article in Special Issue
Electrochemical Sensors for Clinic Analysis
Article

Application of DNA Hybridization Biosensor as a Screening Method for the Detection of Genetically Modified Food Components

Chair of Biochemistry and Microbiology, Poznan University of Economics, Al. Niepodleglosci 10, 60-967 Poznan, Poland
*
Author to whom correspondence should be addressed.
Sensors 2008, 8(4), 2118-2135; https://doi.org/10.3390/s8042118
Received: 29 January 2008 / Accepted: 20 March 2008 / Published: 27 March 2008
An electrochemical biosensor for the detection of genetically modified food components is presented. The biosensor was based on 21-mer single-stranded oligonucleotide (ssDNA probe) specific to either 35S promoter or nos terminator, which are frequently present in transgenic DNA cassettes. ssDNA probe was covalently attached by 5’-phosphate end to amino group of cysteamine self-assembled monolayer (SAM) on gold electrode surface with the use of activating reagents – water soluble 1-ethyl-3(3’- dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxy-sulfosuccinimide (NHS). The hybridization reaction on the electrode surface was detected via methylene blue (MB) presenting higher affinity to ssDNA probe than to DNA duplex. The electrode modification procedure was optimized using 19-mer oligoG and oligoC nucleotides. The biosensor enabled distinction between DNA samples isolated from soybean RoundupReady® (RR soybean) and non-genetically modified soybean. The frequent introduction of investigated DNA sequences in other genetically modified organisms (GMOs) give a broad perspectives for analytical application of the biosensor. View Full-Text
Keywords: DNA biosensor; Self-assembled monolayer; Methylene blue; Roundup Ready soybeam DNA biosensor; Self-assembled monolayer; Methylene blue; Roundup Ready soybeam
Show Figures

MDPI and ACS Style

Tichoniuk, M.; Ligaj, M.; Filipiak, M. Application of DNA Hybridization Biosensor as a Screening Method for the Detection of Genetically Modified Food Components. Sensors 2008, 8, 2118-2135. https://doi.org/10.3390/s8042118

AMA Style

Tichoniuk M, Ligaj M, Filipiak M. Application of DNA Hybridization Biosensor as a Screening Method for the Detection of Genetically Modified Food Components. Sensors. 2008; 8(4):2118-2135. https://doi.org/10.3390/s8042118

Chicago/Turabian Style

Tichoniuk, Mariusz, Marta Ligaj, and Marian Filipiak. 2008. "Application of DNA Hybridization Biosensor as a Screening Method for the Detection of Genetically Modified Food Components" Sensors 8, no. 4: 2118-2135. https://doi.org/10.3390/s8042118

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop