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A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide

Department of Chemistry, Institute of Analytical Chemistry, Liaocheng University, Liaocheng, 252059, China
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Sensors 2005, 5(4), 266-276; https://doi.org/10.3390/s5040266
Received: 25 June 2004 / Accepted: 14 November 2004 / Published: 4 May 2005
(This article belongs to the Special Issue Papers presented at I3S2004, Nanjing)
An amperometric biosensor for hydrogen peroxide (H2O2) was developed viaan easy and effective enzyme immobilization method with the “sandwich” configuration:ferrocene-chitosan: HRP: chitosan-glyoxal using a glassy carbon electrode as the basicelectrode. In order to prevent the loss of immobilized HRP under optimized conditions,the biosensor surface was cross-linked with glyoxal. Ferrocene was selected andimmobilized on the glassy carbon electrode surface as a mediator. The fabricationprocedure was systematically optimized to improve the biosensor performance. Thebiosensor had a fast response of less than 10 s to H2O2, with a linear range of 3.5×10-5 to1.1×10-3 M, and a detection limit of 8.0×10-6 M based on S/N = 3. View Full-Text
Keywords: horseradish peroxidase; biosensor; hydrogen peroxide; chitosan horseradish peroxidase; biosensor; hydrogen peroxide; chitosan
MDPI and ACS Style

Wang, H.-S.; Pan, Q.-X.; Wang, G.-X. A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide. Sensors 2005, 5, 266-276.

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