A Novel NIR-FRET Biosensor for Reporting PS/γ-Secretase Activity in Live Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plasmid DNA
2.2. Cell Culture and Transfection
2.3. Subcellular Fractionation
2.4. Western Blotting
2.5. Aβ ELISA
2.6. LDH Cytotoxicity Assay
2.7. Spectral FRET Analysis
2.8. Statistics
3. Results
3.1. The C99 720-670 Biosensor Is Cleaved by Endogenous PS/γ-Secretase
3.2. The C99 720-670 Biosensor Reports Endogenous PS/γ-Secretase Activity in Live Cells
3.3. The C99 720-670 Biosensor is Compatible with CFP-YFP Based Biosensors
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Houser, M.C.; Hou, S.S.; Perrin, F.; Turchyna, Y.; Bacskai, B.J.; Berezovska, O.; Maesako, M. A Novel NIR-FRET Biosensor for Reporting PS/γ-Secretase Activity in Live Cells. Sensors 2020, 20, 5980. https://doi.org/10.3390/s20215980
Houser MC, Hou SS, Perrin F, Turchyna Y, Bacskai BJ, Berezovska O, Maesako M. A Novel NIR-FRET Biosensor for Reporting PS/γ-Secretase Activity in Live Cells. Sensors. 2020; 20(21):5980. https://doi.org/10.3390/s20215980
Chicago/Turabian StyleHouser, Mei CQ, Steven S Hou, Florian Perrin, Yuliia Turchyna, Brian J Bacskai, Oksana Berezovska, and Masato Maesako. 2020. "A Novel NIR-FRET Biosensor for Reporting PS/γ-Secretase Activity in Live Cells" Sensors 20, no. 21: 5980. https://doi.org/10.3390/s20215980
APA StyleHouser, M. C., Hou, S. S., Perrin, F., Turchyna, Y., Bacskai, B. J., Berezovska, O., & Maesako, M. (2020). A Novel NIR-FRET Biosensor for Reporting PS/γ-Secretase Activity in Live Cells. Sensors, 20(21), 5980. https://doi.org/10.3390/s20215980