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Article

In-Cell Determination of Lactate Dehydrogenase Activity in a Luminal Breast Cancer Model – ex vivo Investigation of Excised Xenograft Tumor Slices Using dDNP Hyperpolarized [1-13C]pyruvate

Department of Radiology, Hadassah Medical Center, Hebrew University of Jerusalem, The Faculty of Medicine, Jerusalem 9112001, Israel
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Sensors 2019, 19(9), 2089; https://doi.org/10.3390/s19092089
Received: 22 March 2019 / Revised: 18 April 2019 / Accepted: 30 April 2019 / Published: 5 May 2019
(This article belongs to the Special Issue Advanced Spectroscopy, Imaging and Sensing in Biomedicine)
[1-13C]pyruvate, the most widely used compound in dissolution-dynamic nuclear polarization (dDNP) magnetic resonance (MR), enables the visualization of lactate dehydrogenase (LDH) activity. This activity had been demonstrated in a wide variety of cancer models, ranging from cultured cells, to xenograft models, to human tumors in situ. Here we quantified the LDH activity in precision cut tumor slices (PCTS) of breast cancer xenografts. The Michigan Cancer Foundation-7 (MCF7) cell-line was chosen as a model for the luminal breast cancer type which is hormone responsive and is highly prevalent. The LDH activity, which was manifested as [1-13C]lactate production in the tumor slices, ranged between 3.8 and 6.1 nmole/nmole adenosine tri-phosphate (ATP) in 1 min (average 4.6 ± 1.0) on three different experimental set-ups consisting of arrested vs. continuous perfusion and non-selective and selective RF pulsation schemes and combinations thereof. This rate was converted to an expected LDH activity in a mass ranging between 3.3 and 5.2 µmole/g in 1 min, using the ATP level of these tumors. This indicated the likely utility of this approach in clinical dDNP of the human breast and may be useful as guidance for treatment response assessment in a large number of tumor types and therapies ex vivo. View Full-Text
Keywords: LDH; 13C-NMR; hyperpolarization; magnetic resonance; breast cancer; precision-cut tissue slices LDH; 13C-NMR; hyperpolarization; magnetic resonance; breast cancer; precision-cut tissue slices
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MDPI and ACS Style

Adler-Levy, Y.; Nardi-Schreiber, A.; Harris, T.; Shaul, D.; Uppala, S.; Sapir, G.; Lev-Cohain, N.; Sosna, J.; Goldberg, S.N.; Gomori, J.M.; Katz-Brull, R. In-Cell Determination of Lactate Dehydrogenase Activity in a Luminal Breast Cancer Model – ex vivo Investigation of Excised Xenograft Tumor Slices Using dDNP Hyperpolarized [1-13C]pyruvate. Sensors 2019, 19, 2089. https://doi.org/10.3390/s19092089

AMA Style

Adler-Levy Y, Nardi-Schreiber A, Harris T, Shaul D, Uppala S, Sapir G, Lev-Cohain N, Sosna J, Goldberg SN, Gomori JM, Katz-Brull R. In-Cell Determination of Lactate Dehydrogenase Activity in a Luminal Breast Cancer Model – ex vivo Investigation of Excised Xenograft Tumor Slices Using dDNP Hyperpolarized [1-13C]pyruvate. Sensors. 2019; 19(9):2089. https://doi.org/10.3390/s19092089

Chicago/Turabian Style

Adler-Levy, Yael, Atara Nardi-Schreiber, Talia Harris, David Shaul, Sivaranjan Uppala, Gal Sapir, Naama Lev-Cohain, Jacob Sosna, Shraga N. Goldberg, J. M. Gomori, and Rachel Katz-Brull. 2019. "In-Cell Determination of Lactate Dehydrogenase Activity in a Luminal Breast Cancer Model – ex vivo Investigation of Excised Xenograft Tumor Slices Using dDNP Hyperpolarized [1-13C]pyruvate" Sensors 19, no. 9: 2089. https://doi.org/10.3390/s19092089

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