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Open AccessArticle

Application of a Portable Multi-Analyte Biosensor for Organic Acid Determination in Silage

Institute of Nano- and Biotechnologies (INB), FH Aachen, Heinrich-Mußmann-Straße 1, 52428 Jülich, Germany
Institute of Pharmaceutical Chemistry, Philipps-Universität Marburg, 35037 Marburg, Germany
Institute of Complex Systems 8 (ICS-8); Forschungszentrum Jülich GmbH, Wilhelm-Johnen-Straße 1, 52425 Jülich, Germany
Author to whom correspondence should be addressed.
Sensors 2018, 18(5), 1470;
Received: 1 March 2018 / Revised: 25 April 2018 / Accepted: 3 May 2018 / Published: 8 May 2018
(This article belongs to the Special Issue I3S 2017 Selected Papers)
Multi-analyte biosensors may offer the opportunity to perform cost-effective and rapid analysis with reduced sample volume, as compared to electrochemical biosensing of each analyte individually. This work describes the development of an enzyme-based biosensor system for multi-parametric determination of four different organic acids. The biosensor array comprises five working electrodes for simultaneous sensing of ethanol, formate, d-lactate, and l-lactate, and an integrated counter electrode. Storage stability of the biosensor was evaluated under different conditions (stored at +4 °C in buffer solution and dry at −21 °C, +4 °C, and room temperature) over a period of 140 days. After repeated and regular application, the individual sensing electrodes exhibited the best stability when stored at −21 °C. Furthermore, measurements in silage samples (maize and sugarcane silage) were conducted with the portable biosensor system. Comparison with a conventional photometric technique demonstrated successful employment for rapid monitoring of complex media. View Full-Text
Keywords: biosensor; ethanol; formate; d-/l-lactate, multi-analyte; silage biosensor; ethanol; formate; d-/l-lactate, multi-analyte; silage
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MDPI and ACS Style

Pilas, J.; Yazici, Y.; Selmer, T.; Keusgen, M.; Schöning, M.J. Application of a Portable Multi-Analyte Biosensor for Organic Acid Determination in Silage. Sensors 2018, 18, 1470.

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