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Sensors 2018, 18(3), 687;

Lab-on-a-Disc Platform for Automated Chemical Cell Lysis

College of Information and Communication Engineering, Sungkyunkwan University, Suwon 440-746, Korea
Author to whom correspondence should be addressed.
Received: 12 January 2018 / Revised: 20 February 2018 / Accepted: 23 February 2018 / Published: 26 February 2018
(This article belongs to the Special Issue Lab-on-a-Chip–From Point of Care to Precision Medicine)
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Chemical cell lysis is an interesting topic in the research to Lab-on-a-Disc (LOD) platforms on account of its perfect compatibility with the centrifugal spin column format. However, standard procedures followed in chemical cell lysis require sophisticated non-contact temperature control as well as the use of pressure resistant valves. These requirements pose a significant challenge thereby making the automation of chemical cell lysis on an LOD extremely difficult to achieve. In this study, an LOD capable of performing fully automated chemical cell lysis is proposed, where a combination of chemical and thermal methods has been used. It comprises a sample inlet, phase change material sheet (PCMS)-based temperature sensor, heating chamber, and pressure resistant valves. The PCMS melts and solidifies at a certain temperature and thus is capable of indicating whether the heating chamber has reached a specific temperature. Compared to conventional cell lysis systems, the proposed system offers advantages of reduced manual labor and a compact structure that can be readily integrated onto an LOD. Experiments using Salmonella typhimurium strains were conducted to confirm the performance of the proposed cell lysis system. The experimental results demonstrate that the proposed system has great potential in realizing chemical cell lysis on an LOD whilst achieving higher throughput in terms of purity and yield of DNA thereby providing a good alternative to conventional cell lysis systems. View Full-Text
Keywords: chemical cell lysis; Lab-on-a-Disc; laser module; pressure resistant valve; phase change material sheet chemical cell lysis; Lab-on-a-Disc; laser module; pressure resistant valve; phase change material sheet

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Seo, M.-J.; Yoo, J.-C. Lab-on-a-Disc Platform for Automated Chemical Cell Lysis. Sensors 2018, 18, 687.

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