A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection
AbstractIn this paper, we present a phase-intensity surface plasmon resonance (SPR) biosensor and demonstrate its use for avian influenza A (H5N1) antibody biomarker detection. The sensor probes the intensity variation produced by the steep phase response at surface plasmon excitation. The prism sensor head is fixed between a pair of polarizers with a perpendicular orientation angle and a forbidden transmission path. At SPR, a steep phase change is introduced between the p- and s-polarized light, and this rotates the polarization ellipse of the transmission beam. This allows the light at resonance to be transmitted and a corresponding intensity change to be detected. Neither time-consuming interference fringe analysis nor a phase extraction process is required. In refractive index sensing experiments, the sensor resolution was determined to be 6.3 × 10−6 refractive index values (RIU). The sensor has been further applied for H5N1 antibody biomarker detection, and the sensor resolution was determined to be 193.3 ng mL−1, compared to 1 μg mL−1 and 0.5 μg mL−1, as reported in literature for influenza antibody detection using commercial Biacore systems. It represents a 517.3% and 258.7% improvement in detection limit, respectively. With the unique features of label-free, real-time, and sensitive detection, the phase-intensity SPR biosensor has promising potential applications in influenza detection. View Full-Text
- Supplementary File 1:
Supplementary (PDF, 413 KB)
Share & Cite This Article
Wong, C.L.; Chua, M.; Mittman, H.; Choo, L.X.; Lim, H.Q.; Olivo, M. A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection. Sensors 2017, 17, 2363.
Wong CL, Chua M, Mittman H, Choo LX, Lim HQ, Olivo M. A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection. Sensors. 2017; 17(10):2363.Chicago/Turabian Style
Wong, Chi Lok; Chua, Marissa; Mittman, Heather; Choo, Li Xian; Lim, Hann Qian; Olivo, Malini. 2017. "A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection." Sensors 17, no. 10: 2363.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.