Quantum Dot-Based Molecular Beacon to Monitor Intracellular MicroRNAs
AbstractFluorescence monitoring of endogenous microRNA (miRNA or miR) activity related to neuronal development using nano-sized materials provides crucial information on miRNA expression patterns in a noninvasive manner. In this study, we report a new method to monitor intracellular miRNA124a using quantum dot-based molecular beacon (R9-QD-miR124a beacon). The R9-QD-miR124a beacon was constructed using QDs and two probes, miR124a-targeting oligomer and arginine rich cell-penetrating peptide (R9 peptide). The miR124a-targeting oligomer contains a miR124a binging sequence and a black hole quencher 1 (BHQ1). In the absence of target miR124a, the R9-QD-miR124a beacon forms a partial duplex beacon and remained in quenched state because the BHQ1 quenches the fluorescence signal of the R9-QD-miR124a beacon. The binding of miR124a to the miR124a binding sequence of the miR124a-targeting oligomer triggered the separation of the BHQ1 quencher and subsequent signal-on of a red fluorescence signal. Moreover, enhanced cellular uptake was achieved by conjugation with the R9 peptide, which resulted in increased fluorescent signal of the R9-QD-miR124a beacons in P19 cells during neurogenesis due to the endogenous expression of miR124a. View Full-Text
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Lee, J.; Moon, S.U.; Lee, Y.S.; Ali, B.A.; Al-Khedhairy, A.A.; Ali, D.; Ahmed, J.; Al Salem, A.M.; Kim, S. Quantum Dot-Based Molecular Beacon to Monitor Intracellular MicroRNAs. Sensors 2015, 15, 12872-12883.
Lee J, Moon SU, Lee YS, Ali BA, Al-Khedhairy AA, Ali D, Ahmed J, Al Salem AM, Kim S. Quantum Dot-Based Molecular Beacon to Monitor Intracellular MicroRNAs. Sensors. 2015; 15(6):12872-12883.Chicago/Turabian Style
Lee, Jonghwan; Moon, Sung U.; Lee, Yong S.; Ali, Bahy A.; Al-Khedhairy, Abdulaziz A.; Ali, Daoud; Ahmed, Javed; Al Salem, Abdullah M.; Kim, Soonhag. 2015. "Quantum Dot-Based Molecular Beacon to Monitor Intracellular MicroRNAs." Sensors 15, no. 6: 12872-12883.