Next Article in Journal
An Uncertainty-Based Distributed Fault Detection Mechanism for Wireless Sensor Networks
Next Article in Special Issue
Metal Oxide Nanosensors Using Polymeric Membranes, Enzymes and Antibody Receptors as Ion and Molecular Recognition Elements
Previous Article in Journal
A Comparative Study of Information-Based Source Number Estimation Methods and Experimental Validations on Mechanical Systems
Previous Article in Special Issue
Development of a Sweetness Sensor for Aspartame, a Positively Charged High-Potency Sweetener
Communication

The First Electrochemical MIP Sensor for Tamoxifen

by 1,2 and 1,2,*
1
Fraunhofer Institute for Biomedical Engineering IBMT, Am Mühlenberg 13, 14476 Potsdam, Germany
2
Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany
*
Author to whom correspondence should be addressed.
Sensors 2014, 14(5), 7647-7654; https://doi.org/10.3390/s140507647
Received: 28 February 2014 / Revised: 11 April 2014 / Accepted: 24 April 2014 / Published: 25 April 2014
(This article belongs to the Special Issue Biomimetic Receptors and Sensors)
We present an electrochemical MIP sensor for tamoxifen (TAM)—a nonsteroidal anti-estrogen—which is based on the electropolymerisation of an O-phenylenediamine‒resorcinol mixture directly on the electrode surface in the presence of the template molecule. Up to now only “bulk” MIPs for TAM have been described in literature, which are applied for separation in chromatography columns. Electro-polymerisation of the monomers in the presence of TAM generated a film which completely suppressed the reduction of ferricyanide. Removal of the template gave a markedly increased ferricyanide signal, which was again suppressed after rebinding as expected for filling of the cavities by target binding. The decrease of the ferricyanide peak of the MIP electrode depended linearly on the TAM concentration between 1 and 100 nM. The TAM-imprinted electrode showed a 2.3 times higher recognition of the template molecule itself as compared to its metabolite 4-hydroxytamoxifen and no cross-reactivity with the anticancer drug doxorubucin was found. Measurements at +1.1 V caused a fouling of the electrode surface, whilst pretreatment of TAM with peroxide in presence of HRP generated an oxidation product which was reducible at 0 mV, thus circumventing the polymer formation and electrochemical interferences. View Full-Text
Keywords: molecularly imprinted polymers; anticancer drug; tamoxifen; electropolymerisation molecularly imprinted polymers; anticancer drug; tamoxifen; electropolymerisation
Show Figures

MDPI and ACS Style

Yarman, A.; Scheller, F.W. The First Electrochemical MIP Sensor for Tamoxifen. Sensors 2014, 14, 7647-7654. https://doi.org/10.3390/s140507647

AMA Style

Yarman A, Scheller FW. The First Electrochemical MIP Sensor for Tamoxifen. Sensors. 2014; 14(5):7647-7654. https://doi.org/10.3390/s140507647

Chicago/Turabian Style

Yarman, Aysu, and Frieder W. Scheller. 2014. "The First Electrochemical MIP Sensor for Tamoxifen" Sensors 14, no. 5: 7647-7654. https://doi.org/10.3390/s140507647

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop