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Sensors 2011, 11(8), 7879-7891; https://doi.org/10.3390/s110807879

Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats

1
Division of Biology, Office of Science and Engineering Laboratories, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA
2
Fischell Department of Bioengineering, University of Maryland, College Park, MD 20993, USA
3
eBioscience, Inc., 10255 Science Center Drive, San Diego, CA 92121, USA
4
Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, 4555 Overlook Avenue, S.W., Washington, DC 20375, USA
5
College of Science, George Mason University, 4400 University Dr., Fairfax, VA 22030, USA
*
Authors to whom correspondence should be addressed.
Received: 25 April 2011 / Revised: 29 July 2011 / Accepted: 1 August 2011 / Published: 11 August 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
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Abstract

The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry using a facile protocol that took View Full-Text
Keywords: quantum dot (QD); nanocrystal (NC); semiconductor; bioconjugation; sensor; multiplex; immunoassay; sulfhydryl chemistry quantum dot (QD); nanocrystal (NC); semiconductor; bioconjugation; sensor; multiplex; immunoassay; sulfhydryl chemistry
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Sapsford, K.E.; Spindel, S.; Jennings, T.; Tao, G.; Triulzi, R.C.; Algar, W.R.; Medintz, I.L. Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats. Sensors 2011, 11, 7879-7891.

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