Next Article in Journal
Pressure Sensing in High-Refractive-Index Liquids Using Long-Period Gratings Nanocoated with Silicon Nitride
Next Article in Special Issue
Direct Detection of the Biological Toxin in Acidic Environment by Electrochemical Impedimetric Immunosensor
Previous Article in Journal
A High-Throughput Enzyme Assay for Organophosphate Residues in Milk
Previous Article in Special Issue
Small Molecule Immunosensing Using Surface Plasmon Resonance
Article Menu

Export Article

Open AccessReview
Sensors 2010, 10(12), 11287-11300;

Ca2+-Regulated Photoproteins: Effective Immunoassay Reporters

Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 50, Krasnoyarsk, 660036, Russia
Received: 29 October 2010 / Revised: 24 November 2010 / Accepted: 3 December 2010 / Published: 10 December 2010
(This article belongs to the Special Issue Immunosensors)
Full-Text   |   PDF [326 KB, uploaded 21 June 2014]


Ca2+-regulated photoproteins of luminous marine coelenterates are of interest and a challenge for researchers as a unique bioluminescent system and as a promising analytical instrument for both in vivo and in vitro applications. The proteins are comprehensively studied as to biochemical properties, tertiary structures, bioluminescence mechanism, etc. This knowledge, along with available recombinant proteins serves the basis for development of unique bioluminescent detection systems that are “self-contained”, triggerable, fast, highly sensitive, and non-hazardous. In the paper, we focus on the use of photoproteins as reporters in binding assays based on immunological recognition element—bioluminescent immunoassay and hybridization immunoassay, their advantages and prospects. View Full-Text
Keywords: bioluminescence; Ca2+-regulated photoprotein; immunoassay; PCR-ELISA; multiplex assay; re-engineered photoproteins bioluminescence; Ca2+-regulated photoprotein; immunoassay; PCR-ELISA; multiplex assay; re-engineered photoproteins
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Frank, L.A. Ca2+-Regulated Photoproteins: Effective Immunoassay Reporters. Sensors 2010, 10, 11287-11300.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top