Recognition Properties and Competitive Assays of a Dual Dopamine/Serotonin Selective Molecularly Imprinted Polymer
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and characterisation of DS-MIP
2.2. Template recognition by DS-MIP
2.3. Binding characteristics of DS-MIP
2.4. The specificity of DS-MIP
2.5. Specificity of the ergot derivatives towards DS-MIP
2.6. Affinity of the ergot derivatives towards DS-MIP
3. Experimental Section
3.1. Chemicals
3.2. Apparatus
3.3. Investigation of template/functional monomer complexation by 1H-NMR spectroscopy
3.4. Polymer synthesis
3.5. Determination of the selective recognition ability of DS-MIP
3.6. Determination of the binding characteristics of DS-MIP
3.7. Determination of the specificity of DS-MIP
3.8. Determination of the binding-reactivity of the ergot derivatives
3.9. Competitive ligand binding assays for the tested ergot derivatives
3.10. Determination of the binding affinities of the ergot derivatives to the natural receptor
3.11. Statistical analysis
4. Conclusions
Acknowledgments
References and Notes
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Polymer | Particle size a) (μm) | Pore diameter (nm) | Pore volume b) (mL/g) | BET Surface area b) (m2/g) |
---|---|---|---|---|
NIP | 29.10 | 52.21 | 0.25 (0.009) | 201.9 (24.6) |
DS-MIP | 22.23 | 18.36 | 0.21 (0.006) | 133.3 (17.5) |
D-MIP c) | 19.93 | 30.17 | 0.24 (0.014) | 130.4 (34.4) |
S-MIP | 19.43 | 21.09 | 0.31 (0.005) | 158.4 (14.8) |
Polymer | Ligand | Bmax (μmol/g) | Kd1 (mM) | Kd2 (mM) |
---|---|---|---|---|
NIP | D | 0.52 ± 0.17 | 1.21 ± 0.23 | 0.55 ± 0.23 |
S | 1.45 ± 0.60 | 3.33 ± 0.73 | 0.24 ± 0.09 | |
DS-MIP | D | 3.10 ± 0.52 | 0.073 ± 0.002 | 10.41 ± 0.21 |
S | 5.71 ± 0.32 | 0.027 ± 0.0003 | 1.67 ± 0.13 | |
D-MIP | D | 3.48 ± 0.70 | 0.094 ± 0.028 | 1.25 ± 0.60 |
S | 4.63 ± 1.32 | 0.196 ± 0.054 | 3.53 ± 1.02 | |
S-MIP | D | 3.34 ± 0.74 | 0.278 ± 0.092 | 10.01 ± 0.36 |
S | 4.65 ± 0.32 | 0.074 ± 0.012 | 14.49 ± 3.25 |
DS-MIP | D-MIP | S-MIP | |
---|---|---|---|
D | |||
Nt | 3.64 ± 0.01 μmol/g | 2.76 ± 0.19 μmol/g | 10.27 ± 0.66 μmol/g |
a | 3.30 ± 0.01 mM−1 | 3.44 ± 0.05 mM−1 | 1.02 ± 0.12 mM−1 |
Ko | 4.17 ± 0.01 mM−1 | 4.48 ± 0.04 mM−1 | 1.03 ± 0.16 mM−1 |
m | 0.84 ± 0.01 | 0.83 ± 0.03 | 0.75 ± 0.02 |
Limits of affinity distribution* | 0.09–9.5 mM−1 | 0.09–9.5 mM−1 | 0.19–9.5 mM−1 |
R2 | 0.998 | 0.966 | 0.975 |
S | |||
Nt | 5.86 ± 0.01 μmol/g | 46.54 ± 16.81 μmol/g | 5.00 ± 0.37 μmol/g |
a | 4.73 ± 0.08 mM−1 | 0.07 ± 0.01 mM−1 | 4.02 ± 0.16 mM−1 |
Ko | 12.85 ± 1.09 mM−1 | 0.03 ± 0.001 mM−1 | 8.08 ± 0.36 mM−1 |
m | 0.61 ± 0.02 | 0.82 ± 0.02 | 0.67 ± 0.01 |
Limits of affinity distribution* | 0.21–25.0 mM−1 | 0.19–9.5 mM−1 | 0.20–14.18 mM−1 |
R2 | 0.976 | 0.993 | 0.997 |
Ergot | Ki (μM)
| |||
---|---|---|---|---|
D ligand | S ligand | |||
DS-MIP a) | Rat hypothalamus b) | DS-MIP a) | Rat hypothalamus b) | |
Terguride | 0.14 ± 0.01 | 0.00003 ± 0.000005 | 7.79 ± 0.17 | 0.0013 ± 0.0001 |
Pergolide | 0.20 ± 0.01 | 0.0007 ± 0.0001 | 12.78 ± 0.09 | 0.0060 ± 0.0002 |
Agroclavine | 0.40 ± 0.01 | 0.0015 ± 0.0002 | 5.31 ± 0.22 | 0.0012 ± 0.0001 |
Ergonovine | 69.57 ± 0.51 | 0.0025 ± 0.0001 | 14.74 ± 0.29 | 0.0012 ± 0.0001 |
Ergocryptine | 124.88 ± 1.22 | 0.0028 ± 0.0002 | 24.85 ± 0.69 | 0.0100 ± 0.0004 |
Ergocristine | 141.08 ± 1.47 | 0.0076 ± 0.0001 | 19.33 ± 0.60 | 0.0068 ± 0.0002 |
Ergocornine | 216.60 ± 2.59 | 0.0104 ± 0.0002 | 27.25 ± 0.78 | 0.0200 ± 0.0001 |
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Suedee, R.; Seechamnanturakit, V.; Suksuwan, A.; Canyuk, B. Recognition Properties and Competitive Assays of a Dual Dopamine/Serotonin Selective Molecularly Imprinted Polymer. Int. J. Mol. Sci. 2008, 9, 2333-2356. https://doi.org/10.3390/ijms9122333
Suedee R, Seechamnanturakit V, Suksuwan A, Canyuk B. Recognition Properties and Competitive Assays of a Dual Dopamine/Serotonin Selective Molecularly Imprinted Polymer. International Journal of Molecular Sciences. 2008; 9(12):2333-2356. https://doi.org/10.3390/ijms9122333
Chicago/Turabian StyleSuedee, Roongnapa, Vatcharee Seechamnanturakit, Acharee Suksuwan, and Bhutorn Canyuk. 2008. "Recognition Properties and Competitive Assays of a Dual Dopamine/Serotonin Selective Molecularly Imprinted Polymer" International Journal of Molecular Sciences 9, no. 12: 2333-2356. https://doi.org/10.3390/ijms9122333