Exon 11 Polymorphism (rs1126797) in the Thyroid Peroxidase (TPO) Gene Among Caucasian Polish Patients with Autoimmune Thyroiditis: A Short Communication
Abstract
1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Patients
4.2. Methods
Genotyping of TPO Exon 11 SNP (rs1126797)
4.3. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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SNP | Allele/Genotype | AIT (n = 234), n (%) | Controls (n = 132), n (%) | p-Value | OR (95% CI) |
---|---|---|---|---|---|
rs1126797 | Genotypes | ||||
CC | 102 (43.59) | 54 (40.91) | 0.83 * 0.54 ** | ||
CT | 106 (45.30) | 61 (46.21) | |||
TT | 26 (11.11) | 17 (12.88) | |||
CC vs. CT + TT | 102 (43.59) vs. 132 (56.41) | 54 (40.91) vs. 78 (59.09) | 0.62 * | 1.12 (0.72–1.72) | |
CC + CT vs. TT | 208 (88.89) vs. 26 (11.11) | 115 (87.12) vs. 17 (12.88) | 0.61 * | 1.18 (0.62–2.27) | |
Alleles | |||||
C allele | 310 (66.24) | 169 (64.02) | 0.54 * | 1.10 (0.80–1.51) | |
T allele | 158 (33.76) | 95 (35.98) |
Author | Population | Statistics |
---|---|---|
Japanese 147 AIT and 92 healthy controls |
| |
Ahmed et al., 2021 [13] | Egyptian 200 AIT and 100 healthy controls |
|
Lacka et al. [present study] | Polish 234 AIT and 132 healthy controls |
|
PCR Reaction Mix (Total Volume: 20 μL) | ||
Component | Volume (μL) | |
Fast Probe qPCR Master Mix 2× | 10.0 | |
Nuclease-Free Water | 8.5 | |
TaqMan SNP Genotyping Assay | 0.5 | |
Genomic DNA | 1.0 | |
PCR Cycling Conditions | ||
Step | Temperature (°C) | Time |
Predenaturation | 95 | 10 min |
Denaturation | 92 | 15 s (at least 40 cycles) |
Annealing/Extension | 60 | 60 s |
Final Extension | 60 | 10 min |
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Lacka, K.; Maciejewski, A.; Łącka, A.M.; Herman, W.; Lacki, J.K.; Żaba, R.; Kowalczyk, M.J. Exon 11 Polymorphism (rs1126797) in the Thyroid Peroxidase (TPO) Gene Among Caucasian Polish Patients with Autoimmune Thyroiditis: A Short Communication. Int. J. Mol. Sci. 2025, 26, 6299. https://doi.org/10.3390/ijms26136299
Lacka K, Maciejewski A, Łącka AM, Herman W, Lacki JK, Żaba R, Kowalczyk MJ. Exon 11 Polymorphism (rs1126797) in the Thyroid Peroxidase (TPO) Gene Among Caucasian Polish Patients with Autoimmune Thyroiditis: A Short Communication. International Journal of Molecular Sciences. 2025; 26(13):6299. https://doi.org/10.3390/ijms26136299
Chicago/Turabian StyleLacka, Katarzyna, Adam Maciejewski, Aleksandra M. Łącka, Waldemar Herman, Jan K. Lacki, Ryszard Żaba, and Michał J. Kowalczyk. 2025. "Exon 11 Polymorphism (rs1126797) in the Thyroid Peroxidase (TPO) Gene Among Caucasian Polish Patients with Autoimmune Thyroiditis: A Short Communication" International Journal of Molecular Sciences 26, no. 13: 6299. https://doi.org/10.3390/ijms26136299
APA StyleLacka, K., Maciejewski, A., Łącka, A. M., Herman, W., Lacki, J. K., Żaba, R., & Kowalczyk, M. J. (2025). Exon 11 Polymorphism (rs1126797) in the Thyroid Peroxidase (TPO) Gene Among Caucasian Polish Patients with Autoimmune Thyroiditis: A Short Communication. International Journal of Molecular Sciences, 26(13), 6299. https://doi.org/10.3390/ijms26136299