Estradiol Promotes Myelin Repair in the Spinal Cord of Female Mice in a CXCR4 Chemokine Receptor-Independent Manner
Abstract
1. Introduction
2. Results
2.1. Spontaneous Remyelination in Female Mice and the Effect of Ovariectomy
2.2. Treatment of Ovariectomized Females with Estradiol or Testosterone Restores Remyelination
2.3. Inhibition of CXCR4 Blocks Testosterone-Dependent Remyelination—But Not Estradiol-Dependent Remyelination
2.4. Estradiol Increases the Number of Oligodendrocytes and Promotes Remyelination in Organotypic Cultures in a CXCR4-Independent Manner
2.5. In the Absence of Estradiol and Oligodendrocyte Remyelination, Schwann Cells Invade and Remyelinate the Demyelinated Lesion
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Focal Demyelination with Lysolecithin (LPC)
4.3. Organotypic Cerebellar Slice Cultures
4.4. Immunohistochemistry
4.5. Immunohistological Analysis
4.6. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Bardy-Lagarde, M.; Asbelaoui, N.; Schumacher, M.; Ghoumari, A.M. Estradiol Promotes Myelin Repair in the Spinal Cord of Female Mice in a CXCR4 Chemokine Receptor-Independent Manner. Int. J. Mol. Sci. 2025, 26, 4752. https://doi.org/10.3390/ijms26104752
Bardy-Lagarde M, Asbelaoui N, Schumacher M, Ghoumari AM. Estradiol Promotes Myelin Repair in the Spinal Cord of Female Mice in a CXCR4 Chemokine Receptor-Independent Manner. International Journal of Molecular Sciences. 2025; 26(10):4752. https://doi.org/10.3390/ijms26104752
Chicago/Turabian StyleBardy-Lagarde, Marianne, Narimene Asbelaoui, Michael Schumacher, and Abdel Mouman Ghoumari. 2025. "Estradiol Promotes Myelin Repair in the Spinal Cord of Female Mice in a CXCR4 Chemokine Receptor-Independent Manner" International Journal of Molecular Sciences 26, no. 10: 4752. https://doi.org/10.3390/ijms26104752
APA StyleBardy-Lagarde, M., Asbelaoui, N., Schumacher, M., & Ghoumari, A. M. (2025). Estradiol Promotes Myelin Repair in the Spinal Cord of Female Mice in a CXCR4 Chemokine Receptor-Independent Manner. International Journal of Molecular Sciences, 26(10), 4752. https://doi.org/10.3390/ijms26104752