TCF7L2 Polymorphism rs7903146 (C/T) and Gestational Diabetes Influence on Obstetric Outcome: A Romanian Case–Control Study
Abstract
1. Introduction
2. Results
2.1. Obstetrical Outcomes
2.2. Genetic Analysis
3. Discussion
4. Materials and Methods
4.1. Study Design
4.2. Blood Collection and Biochemical Assays
4.3. rs7903146 (C/T) Polymorphism Genotyping
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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GDM a (n = 61) | Non-GDM (n = 55) | p-Value d | |
---|---|---|---|
(arithmetic mean ± SD b) | |||
Maternal age (years) | 31.9 ± 3.88 | 30.53 ± 5.07 | 0.102 |
BMI c at admission | 31.49 ± 4.58 | 29.06 ± 4.24 | 0.004 |
Weight gain during pregnancy (kg) | 15.05 ± 5.58 | 15.89 ± 5.24 | 0.418 |
Gestational age at birth (weeks) | 38.44 ± 1.68 | 39.31 ± 1.17 | 0.002 |
Family history of diabetes no (%) | 16 (26.2%) | 8 (14.5%) | 0.119 |
Smoking habit, no (%) | 10 (16.4%) | 2 (3.6%) | 0.021 |
Parity, no (%) | 0.737 | ||
Nulliparous | 42 (68.9%) | 39 (70.9%) | |
Multiparous | 19 (31.1%) | 16 (29.1%) | |
Delivery route no (%) | 0.581 | ||
Vaginal | 29 (47.5%) | 29 (52.7%) | |
C-section | 32 (52.5%) | 26 (47.3%) | |
APGAR score at 5 min | 1.000 | ||
≥7 | 59 (96.7%) | 53 (96.4%) | |
<7 | 2 (3.3%) | 2 (3.6%) | |
Birth weight (centiles) | 0.015 | ||
≤p10 | 3 (4.9%) | 3 (5.5%) | |
p10–p90 | 23 (37.7%) | 35 (63.6%) | |
≤p90 | 35 (57.4%) | 17 (30.9%) |
GDM (N = 61) | Non-GDM (N = 55) | p-Value | |
---|---|---|---|
Maternal complications, (%) | |||
Hypertensive pathology | 12 (19.7%) | 1 (1.8%) | 0.002 |
Premature rupture of membranes | 5 (8.2%) | 0 | 0.059 |
Preterm birth | 6 (9.8%) | 1 (1.8%) | 0.117 |
Polyhydramnios | 5 (8.2%) | 0 | 0.059 |
Failure of labor induction | 7 (11.5%) | 0 | 0.014 |
Shoulder dystocia/soft tissue lacerations | 12 (19.7%) | 1 (1.8%) | 0.002 |
Fetal dystocia | 4 (6.6%) | 1 (1.8%) | 0.367 |
Fetal complications, (%) | |||
IUGR | 3 (4.9%) | 3 (5.5%) | 1.000 |
Macrosomia | 26 (42.6%) | 12 (21.8%) | 0.019 |
Perinatal asphyxia/acute fetal distress | 0 | 2 (3.6%) | 0.223 |
TCF7L2 rs7903146 Genotype | Frequencies | p-Value b | OR [95%CI] c | |
---|---|---|---|---|
GDM a (N = 61) | Non-GDM (N = 55) | |||
CC, no (%) | 23/61 (37.7%) | 28/55 (50.9%) | 0.074 | 0.58 [0.28–1.22] |
CT, no (%) | 23/61 (37.7%) | 22/55 (40%) | 0.91 [0.43–1.92] | |
TT, no (%) | 15/61 (24.6%) | 5/55 (9.1%) | 3.26 [1.10–9.68] | |
Alleles | ||||
C allele, no (%) | 69/122 (56.6%) | 78/110 (70.9%) | 0.016 | 0.53 [0.31–0.92] |
T allele, no (%) | 53/122 (43.3%) | 32/110 (29.1%) | 1.87 [1.09–3.23] | |
Dominant model | ||||
CC, no (%) | 23/61 (37.7%) | 28/55 (50.9%) | 0.107 | 0.58 [0.28–1.22] |
CT+TT, no (%) | 38/61 (62.3%) | 27/55 (49.1%) | 1.71 [0.82–3.59] | |
Recessive model | ||||
TT, no (%) | 15/61 (24.6%) | 5/55 (9.1%) | 0.024 | 3.26 [1.10–9.68] |
CC+CT, no (%) | 46/61 (75.4%) | 50/55 (90.1%) | 0.31 [0.10–0.91] | |
Co-dominant model | ||||
CT, no (%) | 23/61 (37.7%) | 22/55 (40%) | 0.475 | 0.91 [0.43–1.92] |
CC+TT, no (%) | 38/61 (62.3%) | 33/55 (60%) | 1.1 [0.52–2.33] | |
Homozygote | ||||
CC, no (%) | 23/38 (60.5%) | 28/33 (84.8%) | 0.021 | 0.27 [0.09–0.87] |
TT, no (%) | 15/38 (39.5%) | 5/33 (15.2%) | 3.65 [1.15–11.57] | |
Heterozygote | ||||
CC, no (%) | 23/48 (50%) | 28/50 (56%) | 0.351 | 0.79 [0.35–1.76] |
CT, no (%) | 23/48 (40%) | 22/50 (44%) | 1.27 [0.57–2.84] |
GDM Type | Insulin-Requiring GDM | Non-Insulin-Requiring GDM | OR (95% CI) |
---|---|---|---|
CC | 5 (22.7%) | 18 (46.2%) | 0.34 (0.11–1.12) |
CT | 7 (31.8%) | 16 (41%) | 0.67 (0.22–2.02) |
TT | 10 (45.5%) | 5 (12.8%) | 5.67 (1.61–19.97) |
p-value | 0.015 |
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Cruciat, G.; Florian, A.R.; Chaikh-Sulaiman, M.-S.; Staicu, A.; Caracostea, G.V.; Procopciuc, L.M.; Stamatian, F.; Muresan, D. TCF7L2 Polymorphism rs7903146 (C/T) and Gestational Diabetes Influence on Obstetric Outcome: A Romanian Case–Control Study. Int. J. Mol. Sci. 2024, 25, 4039. https://doi.org/10.3390/ijms25074039
Cruciat G, Florian AR, Chaikh-Sulaiman M-S, Staicu A, Caracostea GV, Procopciuc LM, Stamatian F, Muresan D. TCF7L2 Polymorphism rs7903146 (C/T) and Gestational Diabetes Influence on Obstetric Outcome: A Romanian Case–Control Study. International Journal of Molecular Sciences. 2024; 25(7):4039. https://doi.org/10.3390/ijms25074039
Chicago/Turabian StyleCruciat, Gheorghe, Andreea Roxana Florian, Mariam-Suzana Chaikh-Sulaiman, Adelina Staicu, Gabriela Valentina Caracostea, Lucia Maria Procopciuc, Florin Stamatian, and Daniel Muresan. 2024. "TCF7L2 Polymorphism rs7903146 (C/T) and Gestational Diabetes Influence on Obstetric Outcome: A Romanian Case–Control Study" International Journal of Molecular Sciences 25, no. 7: 4039. https://doi.org/10.3390/ijms25074039
APA StyleCruciat, G., Florian, A. R., Chaikh-Sulaiman, M.-S., Staicu, A., Caracostea, G. V., Procopciuc, L. M., Stamatian, F., & Muresan, D. (2024). TCF7L2 Polymorphism rs7903146 (C/T) and Gestational Diabetes Influence on Obstetric Outcome: A Romanian Case–Control Study. International Journal of Molecular Sciences, 25(7), 4039. https://doi.org/10.3390/ijms25074039