Identification of Candidate Male-Reproduction-Related Genes from the Testis and Androgenic Gland of Macrobrachium nipponense, Regulated by PDHE1, through Transcriptome Profiling Analysis
Abstract
:1. Introduction
2. Results
2.1. Measurement of the Efficiency of dsPDHE1
2.2. Transcriptome Profiling Analysis in Testis
2.3. Transcriptome Profiling Analysis in Androgenic Gland
2.4. Selection of Male-Reproduction-Related Genes
2.5. qPCR Analysis of DEGs
3. Materials and Methods
3.1. Sample Preparation
3.2. Synthesize of dsPDHE1 and dsGFP
3.3. RNAi Analysis
3.4. qPCR Analysis
3.5. Transcriptome Profiling Analysis
3.6. Statistical Analysis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Accession Number | Species | E-Value | Fold Change (dsPDHE1 vs. dsGFP) | |
---|---|---|---|---|---|
Testis | Androgenic Gland | ||||
Calcification-associated peptide (Cap) | WGF13344.1 | Macrobrachium nipponense | 6.87 × 10−8 | 21.07 | −10.38 |
Chitin binding-like protein (CBP) | ROT74831.1 | Penaeus vannamei | 3.79 × 10−5 | 3.25 | −2.53 |
Ribosomal protein S3 (RPS3) | KAF6343595.1 | Pipistrellus kuhlii | 0.00011 | −3.12 | −2.78 |
Uncharacterized protein LOC113829596 (UP) | XP_027238594.1 | Penaeus vannamei | 4.28 × 10−6 | 6.89 | 6.37 |
Glucoside xylosyltransferase 2 | XP_045611801.1 | Procambarus clarkii | 8.33 × 10−5 | −21.36 | |
Skeletal muscle actin 6 | QPC96639.1 | Macrobrachium nipponense | 3.61 × 10−5 | −19.23 | |
Polycystic kidney disease protein 1 | XP_047488675.1 | Penaeus chinensis | 4.12 × 10−5 | −13.26 | |
Cytochrome P450 3A30 | XP_037798812.1 | Penaeus monodon | 4.11 × 10−5 | −12.37 | |
Nesprin-1 | XP_045608233.1 | Procambarus clarkii | 5.18 × 10−5 | −10.39 | |
Histidine-rich protein DDB_G0274557 | XP_047473847.1 | Penaeus chinensis | 6.77 × 10−9 | −22.65 | |
Cuticle protein AM1199 | XP_042888222.1 | Penaeus japonicus | 8.87 × 10−9 | −22.47 | |
Cuticle protein 21 | XP_045617846.1 | Procambarus clarkii | 2.58 × 10−5 | −21.50 | |
Pro-resilin-like | XP_047479276.1 | Penaeus chinensis | 4.35 × 10−5 | −19.85 | |
Mucin-19-like | XP_037798457.1 | Penaeus monodon | 0.00012 | −17.23 |
Gene | Primer Sequence |
---|---|
dsPDHE1-F | TAATACGACTCACTATAGGGGTGCTCTTAGCACTGGAGGC |
dsPDHE1-R | TAATACGACTCACTATAGGGCCAAGTAGTGGAAGGCAGGA |
PDHE1-F | TGACCTTAACGGCAACGAGG |
PDHE1-R | TGACCTTAACGGCAACGAGG |
CAP-F | TGGAAGCTCGCCGTAGTTTT |
CAP-R | CTTGACGAAGTGCTGGTTGC |
CBP-F | CAAGGGCGTCTTCGAGTTCT |
CBP-R | AAGTTGCCAGGTTCGGGAAT |
RPS3-F | ATCATGGAGTCTGGCGCAAA |
RPS3-R | ATACCCAGCACACCTTGACG |
UP-F | TGTTGGGCGGGAGTTGAAAT |
UP-R | GTCCTCCTCACCTTGCATCC |
EIF-F | CATGGATGTACCTGTGGTGAAAC |
EIF-R | CATGGATGTACCTGTGGTGAAAC |
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Jin, S.; Xiong, Y.; Zhang, W.; Qiao, H.; Wu, Y.; Jiang, S.; Fu, H. Identification of Candidate Male-Reproduction-Related Genes from the Testis and Androgenic Gland of Macrobrachium nipponense, Regulated by PDHE1, through Transcriptome Profiling Analysis. Int. J. Mol. Sci. 2024, 25, 1940. https://doi.org/10.3390/ijms25031940
Jin S, Xiong Y, Zhang W, Qiao H, Wu Y, Jiang S, Fu H. Identification of Candidate Male-Reproduction-Related Genes from the Testis and Androgenic Gland of Macrobrachium nipponense, Regulated by PDHE1, through Transcriptome Profiling Analysis. International Journal of Molecular Sciences. 2024; 25(3):1940. https://doi.org/10.3390/ijms25031940
Chicago/Turabian StyleJin, Shubo, Yiwei Xiong, Wenyi Zhang, Hui Qiao, Yan Wu, Sufei Jiang, and Hongtuo Fu. 2024. "Identification of Candidate Male-Reproduction-Related Genes from the Testis and Androgenic Gland of Macrobrachium nipponense, Regulated by PDHE1, through Transcriptome Profiling Analysis" International Journal of Molecular Sciences 25, no. 3: 1940. https://doi.org/10.3390/ijms25031940