1,6-Hexanediol Is Inducing Homologous Recombination by Releasing BLM from Assemblysomes in Drosophila melanogaster
Abstract
:1. Introduction
2. Results
2.1. 1,6-Hexanediol Is Less Toxic If Larvae Are Treated with X-ray
2.2. Bloom Syndrome Helicase RNA Containing Assemblysomes Switches to Translating Ribosomes in Response to X-ray or HEX Treatment
2.3. The SMC5/6 Complex Is Involved in the Recruitment of Assemblysome-Regulated DNA Repair Machinery to the Site of DNA Damage
2.4. UV-Induced DNA Damage Is Additive to HEX’ Effect on Mortality
3. Discussion
4. Materials and Methods
4.1. Genotypes, Markers, Crosses, and Strains
4.2. Somatic Mutation and Recombination Test (SMART)
4.3. Larval Treatments
4.4. Isolation of Assemblysomes
4.5. RNA Extraction—Quantitative Real-Time Polymerase Chain Reaction
4.6. Chemicals
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Abbreviations
Sgs1 | Slow growth suppressor 1 |
BLM | Bloom syndrome helicase |
SMC5 | Structural maintenance of chromosomes protein 5 |
HR | Homologous recombination |
DmBLM | Drosophila melanogaster bloom syndrome helicase |
P-bodies | Processing bodies |
SGs | Stress granules |
mwh | multiple wing hairs |
HEX | 1,6-hexanediol |
RNCs | Ribosome nascent chain complexes |
qPCR | quantitative polymerase chain reaction |
EDTA | Ethylenediaminetetraacetic acid |
UAS | Upstream activation sequence |
RNAi | Ribonucleic acid interference |
DSBs | Double-strand breaks |
SMART | Somatic Mutation and Recombination Test |
flr | flare |
Ser | Serrate |
TM3 | Third Multiple 3 |
UV | Ultraviolet |
RT | Reverse transcription |
LLPS | Liquid–liquid phase separation |
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Control | HEX | X-ray Treatment | HEX+X-ray Treatment | |
---|---|---|---|---|
Number of treated larvae | 150 | 150 | 150 | 150 |
Number of mosaic spots (mwh) between mutants | 2 | 4 | 25 | 40 |
Number of mwh/TM3,Ser survivals | 31 | 27 | 19 | 32 |
Number of mwh/+ survivals | 97 | 25 | 55 | 36 |
Number of examined wing pairs (all survivals) | 128 | 52 | 74 | 68 |
Average size of mwh spots in trans-heterozygotes ± standard deviation | 3 ± 0 | 4.5 ± 3.4 | 27.6 ± 43.1 | 16.5 ± 30.9 |
Frequency of mwh spots (mwh) | 0.0103 | 0.08 | 0.2273 | 0.5556 |
Recombination frequency | 1.0309 × 10−6 | 1.0667 × 10−5 | 2.0939 × 10−4 | 3.0602 × 10−4 |
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Gombás, B.G.; Villányi, Z. 1,6-Hexanediol Is Inducing Homologous Recombination by Releasing BLM from Assemblysomes in Drosophila melanogaster. Int. J. Mol. Sci. 2024, 25, 1611. https://doi.org/10.3390/ijms25031611
Gombás BG, Villányi Z. 1,6-Hexanediol Is Inducing Homologous Recombination by Releasing BLM from Assemblysomes in Drosophila melanogaster. International Journal of Molecular Sciences. 2024; 25(3):1611. https://doi.org/10.3390/ijms25031611
Chicago/Turabian StyleGombás, Bence György, and Zoltán Villányi. 2024. "1,6-Hexanediol Is Inducing Homologous Recombination by Releasing BLM from Assemblysomes in Drosophila melanogaster" International Journal of Molecular Sciences 25, no. 3: 1611. https://doi.org/10.3390/ijms25031611