Molecular Characteristics of Circ_002156 and Its Effects on Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells
Abstract
1. Introduction
2. Results
2.1. Characteristics and Cellular Localization of Caprine Circ_002156
2.2. The Tissue Expression Characteristics of Circ_002156 and Its Parent Genes
2.3. The Expression Levels of Circ_002156 and Its Parent Genes in Caprine SMSCs
2.4. Circ_002156 Inhibits Viability of SMSCs
2.5. Circ_002156 Inhibits Proliferation of SMSCs
2.6. Circ_002156 Promotes Differentiation of SMSCs
3. Discussion
4. Materials and Methods
4.1. Sample Collection
4.2. Cell Culture
4.3. The Authenticity Validation of Circ_002156
4.4. Cellular Localization of Circ_002156 in SMSCs
4.5. RNA Isolation and RT-qPCR
4.6. Transfection of Circ_002156 into SMSCs
4.7. Viability and Proliferation of SMSCs Detected
4.8. Effect of Circ_002156 on Differentiation of SMSCs
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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circRNA | Nuclei/Myotube |
---|---|
si-NC | 45.976 ± 1.100 ** |
si-circ_002156 | 28.562 ± 2.276 |
Name | Forward (5′→3′) | Reverse (5′→3′) |
---|---|---|
circ_002156 | F: CTGAACTGACGGCCAAGAAG | R: TCATCCAGACCTGCCATCTC |
MYH2 | F: CTGGCTGGAGAAGAACAAGG | R: CACCGTCTGGAAAGAAGAGC |
MYH1 | F: ATGCGGGAACAGTGGACTAC | R: GCACCAGAGAACAGGAAAGC |
MYH4 | F: TCAAGGGGAGATCACAGTCC | R: GAGGTAGGCAGCCTTGTCAG |
CDK2 | F: GACCAGCTCTTCCGGATCTT | R: ACAAGCTCCGTCCATCTTCA |
CDK4 | F: ACTTTGTGGCCCTCAAGAGT | R: CCTGAGGTCTTGGTCCACAT |
PCNA | F: GAACCTCACCAGCATGTCCAA | R: TTCACCAGAAGGCATCTTTACT |
CyclinE | F: CTCCCTGATTCCCACACCTG | R: CATAAGATGCTTGTCCCTCA |
p53 | F: CGGCTTGCAGAAACCTCTTT | R: CCCTTTTCTACCTCCTGCCA |
MYHC | F: CCACATCTTCTCCATCTCTG | R: GGTTCCTCCTTCTTCTTCTC |
MYOD | F: GTGCAAACGCAAGACGACTA | R: GCTGGTTTGGGTTGCTAGAC |
MYOG | F: CGTGGGCGTGTAAGGTGT | R: GGCGCTCTATGTACTGGATGG |
MEF2C | F: ATCCTGATGCAGACGATTCAG | R: GGTGGAACAGCACACAATCTT |
β-tubulin | F: AGCGTATCTCAGAGCAGTTC | R: AATCCTCTTCCTCTTCTGCG |
GAPDH | F: ACACTGAGGACCAGGTTGTG | R: GACAAAGTGGTCGTTGAGGG |
β-actin | F: AGCCTTCCTTCCTGGGCATGGA | R: GGACAGCACCGTGTTGGCGTAA |
U6 | F: GGAACGATACAGAGAAGATTAGC | R: TGGAACGCTTCACGAATTTGCG |
si-circ_002156 | F: GAGAACAAGGUGAAAAACCTT | R: TGGAACGCTTCACGAATTTGCG |
si-NC | F: UUCUCCGAACGUGUCACGUTT | R: GGUUUUUCACCUUGUUCUCTT |
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Gu, Y.; Shen, J.; Hao, Z.; Zhen, H.; Wu, X.; Wang, J.; Li, M.; Ren, C.; Liu, Y.; Zhao, Y.; et al. Molecular Characteristics of Circ_002156 and Its Effects on Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells. Int. J. Mol. Sci. 2024, 25, 12745. https://doi.org/10.3390/ijms252312745
Gu Y, Shen J, Hao Z, Zhen H, Wu X, Wang J, Li M, Ren C, Liu Y, Zhao Y, et al. Molecular Characteristics of Circ_002156 and Its Effects on Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells. International Journal of Molecular Sciences. 2024; 25(23):12745. https://doi.org/10.3390/ijms252312745
Chicago/Turabian StyleGu, Yuanhua, Jiyuan Shen, Zhiyun Hao, Huimin Zhen, Xinmiao Wu, Jiqing Wang, Mingna Li, Chunyan Ren, Yuan Liu, Yuan Zhao, and et al. 2024. "Molecular Characteristics of Circ_002156 and Its Effects on Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells" International Journal of Molecular Sciences 25, no. 23: 12745. https://doi.org/10.3390/ijms252312745
APA StyleGu, Y., Shen, J., Hao, Z., Zhen, H., Wu, X., Wang, J., Li, M., Ren, C., Liu, Y., Zhao, Y., Yang, P., & Wang, X. (2024). Molecular Characteristics of Circ_002156 and Its Effects on Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells. International Journal of Molecular Sciences, 25(23), 12745. https://doi.org/10.3390/ijms252312745