Evaluation of a Novel Immunochromatographic Device for Detecting Porphyromonas gingivalis in Patients with Periodontal Disease
Abstract
1. Introduction
2. Results
Comparison of a Novel Immunochromatographic Device and Real-Time PCR Method in P. gingivalis Detection
3. Discussion
4. Materials and Methods
4.1. Study Population
4.2. Subgingival Plaque Sampling
4.3. Immunochromatographic Device for P. gingivalis
4.4. Real-Time PCR Method
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Comparison of the Novel Immunochromatographic Device and Real-Time PCR (Real-Time PCR) in Subgingival Plaque Samples | |||||||||
---|---|---|---|---|---|---|---|---|---|
real-time PCR | sensitivity | specificity | PPV | NPV | accuracy | ||||
POS | NEG | TOTAL | 77% | 98% | 94% | 89% | 90% | ||
TEST DEVICE | POS | 33 | 2 | 35 | |||||
NEG | 10 | 80 | 90 | ||||||
TOTAL | 43 | 82 | 88 | ||||||
X2 (p value) | p < 0.01 |
Parameters | Device Score (Test Device) | Number of P. gingivalis (Real-Time PCR) |
---|---|---|
PPD (mm) | 0.612 | 0.594 |
CAL (mm) | 0.605 | 0.606 |
BOP | 0.403 | 0.365 |
Healthy Subjects | Patients with Periodontitis | Difference | |
---|---|---|---|
Number Male Female | n = 53 23 30 | n = 72 36 36 | |
Age (average) | 47.51 ± 21.10 | 69.81 ± 11.82 | p < 0.01 |
PPD (mm) | 2.32 ± 0.51 | 4.96 ± 1.54 | p < 0.01 |
CAL (mm) | 0.74 ± 1.07 | 5.60 ± 1.97 | p < 0.01 |
BOP | 0.06 ± 0.23 | 0.40 ± 0.49 | p < 0.01 |
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Yamanaka, R.; Usui, M.; Kobayashi, K.; Onizuka, S.; Kasai, S.; Sano, K.; Hironaka, S.; Yamasaki, R.; Yoshii, S.; Sato, T.; et al. Evaluation of a Novel Immunochromatographic Device for Detecting Porphyromonas gingivalis in Patients with Periodontal Disease. Int. J. Mol. Sci. 2024, 25, 8187. https://doi.org/10.3390/ijms25158187
Yamanaka R, Usui M, Kobayashi K, Onizuka S, Kasai S, Sano K, Hironaka S, Yamasaki R, Yoshii S, Sato T, et al. Evaluation of a Novel Immunochromatographic Device for Detecting Porphyromonas gingivalis in Patients with Periodontal Disease. International Journal of Molecular Sciences. 2024; 25(15):8187. https://doi.org/10.3390/ijms25158187
Chicago/Turabian StyleYamanaka, Rieko, Michihiko Usui, Kaoru Kobayashi, Satoru Onizuka, Shingo Kasai, Kotaro Sano, Shou Hironaka, Ryota Yamasaki, Shinji Yoshii, Tsuyoshi Sato, and et al. 2024. "Evaluation of a Novel Immunochromatographic Device for Detecting Porphyromonas gingivalis in Patients with Periodontal Disease" International Journal of Molecular Sciences 25, no. 15: 8187. https://doi.org/10.3390/ijms25158187
APA StyleYamanaka, R., Usui, M., Kobayashi, K., Onizuka, S., Kasai, S., Sano, K., Hironaka, S., Yamasaki, R., Yoshii, S., Sato, T., Fujii, W., Iwasaki, M., Ariyoshi, W., Nakashima, K., & Nishihara, T. (2024). Evaluation of a Novel Immunochromatographic Device for Detecting Porphyromonas gingivalis in Patients with Periodontal Disease. International Journal of Molecular Sciences, 25(15), 8187. https://doi.org/10.3390/ijms25158187