The miR-182-5p/GPX4 Pathway Contributes to Sevoflurane-Induced Ototoxicity via Ferroptosis
Abstract
1. Introduction
2. Results
2.1. Fer-1 Attenuated the Ototoxic Effects of Sevoflurane in Mice
2.2. Fer-1 Attenuated Sevoflurane-Induced Ototoxic Effects in Cochlear Explants
2.3. Sevoflurane-Induced Ferroptosis in Cochlear Explants
2.4. Sevoflurane-Induced Ferroptosis in HEI-OC1 Cells
2.5. MiR-182-5p Targeted GPX4 in HEI-OC1 Cells
2.6. Inhibition of miR-182-5p Attenuated Sevoflurane-Induced Ferroptosis in HEI-OC1 Cells
3. Discussion
4. Materials and Methods
4.1. Animals and Anesthesia Treatment
4.2. Cochlear Explant Culture and Treatment
4.3. HEI-OC1 Cell Culture and Treatment
4.4. Acquisition of Mouse Cochlear Basement Membrane
4.5. Immunofluorescence Staining
4.6. Cell Viability Test
4.7. Fe2+ Measurement
4.8. ROS Measurement
4.9. RNA Isolation and Quantitative Real-Time PCR
4.10. Western Blot Analysis
4.11. Dual Luciferase Reporter Gene Assay
4.12. ABR Test
4.13. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jin, L.; Yu, X.; Zhou, X.; Li, G.; Li, W.; He, Y.; Li, H.; Shen, X. The miR-182-5p/GPX4 Pathway Contributes to Sevoflurane-Induced Ototoxicity via Ferroptosis. Int. J. Mol. Sci. 2024, 25, 6774. https://doi.org/10.3390/ijms25126774
Jin L, Yu X, Zhou X, Li G, Li W, He Y, Li H, Shen X. The miR-182-5p/GPX4 Pathway Contributes to Sevoflurane-Induced Ototoxicity via Ferroptosis. International Journal of Molecular Sciences. 2024; 25(12):6774. https://doi.org/10.3390/ijms25126774
Chicago/Turabian StyleJin, Lin, Xiaopei Yu, Xuehua Zhou, Gang Li, Wen Li, Yingzi He, Huawei Li, and Xia Shen. 2024. "The miR-182-5p/GPX4 Pathway Contributes to Sevoflurane-Induced Ototoxicity via Ferroptosis" International Journal of Molecular Sciences 25, no. 12: 6774. https://doi.org/10.3390/ijms25126774
APA StyleJin, L., Yu, X., Zhou, X., Li, G., Li, W., He, Y., Li, H., & Shen, X. (2024). The miR-182-5p/GPX4 Pathway Contributes to Sevoflurane-Induced Ototoxicity via Ferroptosis. International Journal of Molecular Sciences, 25(12), 6774. https://doi.org/10.3390/ijms25126774