Next Article in Journal
Analyzing the Functional Roles and Immunological Features of Chemokines in COAD
Previous Article in Journal
Integrating Genome-Scale Metabolic Models with Patient Plasma Metabolome to Study Endothelial Metabolism In Situ
Previous Article in Special Issue
Metabolic Crossroads: Unveiling the Complex Interactions between Obstructive Sleep Apnoea and Metabolic Syndrome
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Association of Glutathione Peroxidase 3 (GPx3) and miR-196a with Carbohydrate Metabolism Disorders in the Elderly

by
Adam Włodarski
1,
Izabela Szymczak-Pajor
1,
Jacek Kasznicki
2,
Egle Morta Antanaviciute
3,
Bożena Szymańska
4 and
Agnieszka Śliwińska
1,*
1
Department of Nucleic Acid Biochemistry, Medical University of Lodz, 92-213 Lodz, Poland
2
Department of Internal Diseases, Diabetology and Clinical Pharmacology, Medical University of Lodz, 92-213 Lodz, Poland
3
Centre for Cellular Microenvironments, Mazumdar-Shaw Advanced Research Centre, University of Glasgow, Glasgow G12 8QQ, UK
4
Research Laboratory CoreLab, Medical University of Lodz, Mazowiecka 6/8 St., 92-215 Lodz, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(10), 5409; https://doi.org/10.3390/ijms25105409
Submission received: 10 April 2024 / Revised: 7 May 2024 / Accepted: 14 May 2024 / Published: 15 May 2024
(This article belongs to the Special Issue Current Research on Diabetes and Metabolic Syndrome)

Abstract

The escalating prevalence of carbohydrate metabolism disorders (CMDs) prompts the need for early diagnosis and effective markers for their prediction. Hyperglycemia, the primary indicator of CMDs including prediabetes and type 2 diabetes mellitus (T2DM), leads to overproduction of reactive oxygen species (ROS) and oxidative stress (OxS). This condition, resulting from chronic hyperglycemia and insufficient antioxidant defense, causes damage to biomolecules, triggering diabetes complications. Additionally, aging itself can serve as a source of OxS due to the weakening of antioxidant defense mechanisms. Notably, previous research indicates that miR-196a, by downregulating glutathione peroxidase 3 (GPx3), contributes to insulin resistance (IR). Additionally, a GPx3 decrease is observed in overweight/obese and insulin-resistant individuals and in the elderly population. This study investigates plasma GPx3 levels and miR-196a expression as potential CMD risk indicators. We used ELISA to measure GPx3 and qRT-PCR for miR-196a expression, supplemented by multivariate linear regression and receiver operating characteristic (ROC) analysis. Our findings included a significant GPx3 reduction in the CMD patients (n = 126), especially in the T2DM patients (n = 51), and a decreasing trend in the prediabetes group (n = 37). miR-196a expression, although higher in the CMD and T2DM groups than in the controls, was not statistically significant, potentially due to the small sample size. In the individuals with CMD, GPx3 levels exhibited a negative correlation with the mass of adipose tissue, muscle, and total body water, while miR-196a positively correlated with fat mass. In the CMD group, the analysis revealed a weak negative correlation between glucose and GPx3 levels. ROC analysis indicated a 5.2-fold increased CMD risk with GPx3 below 419.501 ng/mL. Logistic regression suggested that each 100 ng/mL GPx3 increase corresponded to a roughly 20% lower CMD risk (OR = 0.998; 95% CI: 0.996–0.999; p = 0.031). These results support the potential of GPx3 as a biomarker for CMD, particularly in T2DM, and the lack of a significant decline in GPx3 levels in prediabetic individuals suggests that it may not serve reliably as an early indicator of CMDs, warranting further large-scale validation.
Keywords: microRNA-196a; GPx3; prediabetes; oxidative stress; biomarker; obesity; insulin resistance; diabetes; hyperglycemia microRNA-196a; GPx3; prediabetes; oxidative stress; biomarker; obesity; insulin resistance; diabetes; hyperglycemia

Share and Cite

MDPI and ACS Style

Włodarski, A.; Szymczak-Pajor, I.; Kasznicki, J.; Antanaviciute, E.M.; Szymańska, B.; Śliwińska, A. Association of Glutathione Peroxidase 3 (GPx3) and miR-196a with Carbohydrate Metabolism Disorders in the Elderly. Int. J. Mol. Sci. 2024, 25, 5409. https://doi.org/10.3390/ijms25105409

AMA Style

Włodarski A, Szymczak-Pajor I, Kasznicki J, Antanaviciute EM, Szymańska B, Śliwińska A. Association of Glutathione Peroxidase 3 (GPx3) and miR-196a with Carbohydrate Metabolism Disorders in the Elderly. International Journal of Molecular Sciences. 2024; 25(10):5409. https://doi.org/10.3390/ijms25105409

Chicago/Turabian Style

Włodarski, Adam, Izabela Szymczak-Pajor, Jacek Kasznicki, Egle Morta Antanaviciute, Bożena Szymańska, and Agnieszka Śliwińska. 2024. "Association of Glutathione Peroxidase 3 (GPx3) and miR-196a with Carbohydrate Metabolism Disorders in the Elderly" International Journal of Molecular Sciences 25, no. 10: 5409. https://doi.org/10.3390/ijms25105409

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop