Microglial FABP4-UCP2 Axis Modulates Neuroinflammation and Cognitive Decline in Obese Mice
Abstract
:1. Introduction
2. Results
2.1. HFD Increases Fat Mass, Lean Mass, Body Weight, Fat/Lean Ratio, and Body Fat Percentage
2.2. HFD Reduces Locomotor Activity
2.3. Loss of FABP4 Protects against Diet-Induced Cognitive Decline
2.4. Loss of FABP4 Leads to Decrease in Hippocampal IBA1 Expression in Mice Fed HFD
2.5. Loss of FABP4 Protects against HFD-Induced Increase in Hippocampal Inflammatory Cytokine Expression
2.6. Loss of FABP4 Alleviates Decrease in Microglial UCP2 mRNA Caused by HFD
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Body Composition
4.3. Locomotor Activity
4.4. Barnes Maze
4.5. T-Maze Spontaneous Alternation
4.6. Immunohistochemistry
4.7. Inflammatory Cytokine Marker Screening
4.8. Real-Time PCR
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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So, S.W.; Fleming, K.M.; Duffy, C.M.; Nixon, J.P.; Bernlohr, D.A.; Butterick, T.A. Microglial FABP4-UCP2 Axis Modulates Neuroinflammation and Cognitive Decline in Obese Mice. Int. J. Mol. Sci. 2022, 23, 4354. https://doi.org/10.3390/ijms23084354
So SW, Fleming KM, Duffy CM, Nixon JP, Bernlohr DA, Butterick TA. Microglial FABP4-UCP2 Axis Modulates Neuroinflammation and Cognitive Decline in Obese Mice. International Journal of Molecular Sciences. 2022; 23(8):4354. https://doi.org/10.3390/ijms23084354
Chicago/Turabian StyleSo, Simon W., Kendra M. Fleming, Cayla M. Duffy, Joshua P. Nixon, David A. Bernlohr, and Tammy A. Butterick. 2022. "Microglial FABP4-UCP2 Axis Modulates Neuroinflammation and Cognitive Decline in Obese Mice" International Journal of Molecular Sciences 23, no. 8: 4354. https://doi.org/10.3390/ijms23084354