Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers
Abstract
:1. Introduction
2. Results
2.1. Purification of Extracellular Vesicles
2.2. Size and ZP Profile of Purified EV Samples
2.3. EV Array Phenotyping
2.4. Small RNA Profile of EVs
2.5. Differentially Enriched miRNAs
3. Discussion
4. Materials and Methods
4.1. Patient Groups and Blood Serum Samples
4.2. Purification of Extracellular Vesicles
4.3. Nanoparticle Tracking Analysis
4.4. Western Blot Analysis
4.5. Transmission Electron Microscopy
4.6. Multiplexed Phenotyping of EVs by EV Array
4.7. Small RNA Sequencing
4.8. Statistical Analysis
4.8.1. NTA Data
4.8.2. EV Array Data
4.8.3. Small RNA Sequencing Data
4.8.4. Data Visualization
4.9. Experimental Design
4.9.1. Investigating the Physical Characteristics of Blood Serum-Derived EVs
4.9.2. EV miRNA and Surface Proteome Profiling of Patient and Control Groups
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Comparison | miRNA | FC | p-Value | FDR |
---|---|---|---|---|
PsA to PsV | QXBT12 | 0.46 | 0.00053 | 0.058 |
hsa-miR-33a-5p | 1.82 | 0.000547 | 0.058 | |
hsa-miR-26a-5p | 1.36 | 0.00101 | 0.0596 | |
hsa-miR-mizuguchi-225 | 1.81 | 0.00113 | 0.0596 | |
miTC3 | 0.43 | 0.00242 | 0.0879 | |
hsa-miR-338-5p | 2.32 | 0.00273 | 0.0879 | |
hsa-miR-671-3p | 0.43 | 0.0029 | 0.0879 | |
hsa-miR-342-3p | 0.52 | 0.00375 | 0.0993 | |
PsA to C | hsa-miR-10b-5p | 0.46 | 0.000204 | 0.0433 |
hsa-miR-197-3p | 0.53 | 0.000873 | 0.0811 | |
hsa-miR-425-5p | 1.51 | 0.00128 | 0.0811 | |
hsa-miR-199a-5p | 1.57 | 0.00153 | 0.0811 | |
hsa-miR-203a-3p | 0.35 | 0.002 | 0.0849 | |
hsa-miR-10a-5p | 0.54 | 0.0025 | 0.0882 | |
hsa-miR-34a-5p | 0.46 | 0.00292 | 0.0884 | |
PsV to C | hsa-miR-423-5p | 2.09 | 3.58 × 10−5 | 0.00419 |
hsa-miR-335-5p | 2.29 | 6.08 × 10−5 | 0.00419 | |
hsa-miR-342-3p | 2.57 | 6.52 × 10−5 | 0.00419 | |
miTC1 | 2.90 | 7.91 × 10−5 | 0.00419 | |
has-miR-425-5p | 1.62 | 0.000183 | 0.00775 | |
hsa-miR-99b-5p | 0.56 | 0.000898 | 0.0317 | |
hsa-miR-17-5p | 1.44 | 0.00148 | 0.0384 | |
hsa-miR-18a-5p | 1.55 | 0.00176 | 0.0384 | |
hsa-miR-27a-3p | 0.72 | 0.0018 | 0.0384 | |
hsa-miR-451a | 1.77 | 0.00181 | 0.0384 | |
QXBT12 | 2.01 | 0.00204 | 0.0394 | |
miTC3 | 2.48 | 0.00279 | 0.0493 | |
hsa-miR-6803-3p | 1.94 | 0.00459 | 0.0697 | |
hsa-miR-199a-5p | 1.50 | 0.0046 | 0.0697 | |
hsa-miR-30b-5p | 0.67 | 0.00554 | 0.0783 | |
hsa-miR-20a-5p | 1.37 | 0.00595 | 0.0789 | |
hsa-miR-340-3p | 1.93 | 0.00641 | 0.0799 | |
hsa-miR-30d-5p | 0.71 | 0.00728 | 0.0858 |
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Lättekivi, F.; Guljavina, I.; Midekessa, G.; Viil, J.; Heath, P.R.; Bæk, R.; Jørgensen, M.M.; Andronowska, A.; Kingo, K.; Fazeli, A. Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers. Int. J. Mol. Sci. 2022, 23, 4005. https://doi.org/10.3390/ijms23074005
Lättekivi F, Guljavina I, Midekessa G, Viil J, Heath PR, Bæk R, Jørgensen MM, Andronowska A, Kingo K, Fazeli A. Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers. International Journal of Molecular Sciences. 2022; 23(7):4005. https://doi.org/10.3390/ijms23074005
Chicago/Turabian StyleLättekivi, Freddy, Irina Guljavina, Getnet Midekessa, Janeli Viil, Paul R. Heath, Rikke Bæk, Malene Møller Jørgensen, Aneta Andronowska, Kulli Kingo, and Alireza Fazeli. 2022. "Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers" International Journal of Molecular Sciences 23, no. 7: 4005. https://doi.org/10.3390/ijms23074005