Benzyl Isothiocyanate Attenuates Inflammasome Activation in Pseudomonas aeruginosa LPS-Stimulated THP-1 Cells and Exerts Regulation through the MAPKs/NF-&kappa;B Pathway

Park, Won Sun; Lee, Jeonghan; Na, Giyoun; Park, SaeGwang; Seo, Su-Kil; Choi, Jung Sik; Jung, Won-Kyo; Choi, Il-Whan

doi:10.3390/ijms23031228

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Benzyl Isothiocyanate Attenuates Inflammasome Activation in Pseudomonas aeruginosa LPS-Stimulated THP-1 Cells and Exerts Regulation through the MAPKs/NF-κB Pathway

^1,†,

^2,†

³,

³,

³,

⁴,

⁵

and

Il-Whan Choi

^3,*

Department of Physiology, Kangwon National University School of Medicine, Chuncheon 24341, Korea

Department of Anesthesiology and Pain Medicine, Busan Paik Hospital, Inje University, Busan 47392, Korea

Department of Microbiology and Immunology, College of Medicine, Inje University, Busan 47392, Korea

⁴

Department of Internal Medicine, Busan Paik Hospital, Inje University, Busan 47392, Korea

⁵

Department of Biomedical Engineering, Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 48513, Korea

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Academic Editor: Giuseppe Pugliese

Int. J. Mol. Sci. 2022, 23(3), 1228; https://doi.org/10.3390/ijms23031228

Received: 24 December 2021 / Revised: 17 January 2022 / Accepted: 20 January 2022 / Published: 22 January 2022

(This article belongs to the Section Molecular Immunology)

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Abstract

Inflammasomes are a group of intracellular multiprotein platforms that play important roles in immune systems. Benzyl isothiocyanate (BITC) is a constituent of cruciferous plants and has been confirmed to exhibit various biological activities. The modulatory effects of BITC on inflammasome-mediated interleukin (IL)-1β expression and its regulatory mechanisms in Pseudomonas aeruginosa (P. aeruginosa) LPS/ATP-stimulated THP-1 cells was investigated. Monocytic THP-1 cells were treated with phorbol myristate acetate (PMA) to induce differentiation into macrophages. Enzyme-linked immunosorbent assays (ELISA) were performed to measure the levels of IL-1β produced in P. aeruginosa LPS/ATP-exposed THP-1 cells. Western blotting was performed to examine the BITC modulatory mechanisms in inflammasome-mediated signaling pathways. BITC inhibited IL-1β production in P. aeruginosa LPS/ATP-induced THP-1 cells. BITC also inhibited activation of leucine-rich repeat protein-3 (NLRP3) and caspase-1 in P. aeruginosa LPS/ATP-induced THP-1 cells. Furthermore, we show that mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) activation in P. aeruginosa LPS was attenuated by BITC. These BITC-mediated modulatory effects on IL-1β production may have therapeutic potential for inflammasome-mediated disorders such as a nasal polyp. View Full-Text

Keywords: inflammasomes; benzyl isothiocyanate; interleukin-1β; Pseudomonas aeruginosa; THP-1 cells; nuclear factor-κB

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Park, W.S.; Lee, J.; Na, G.; Park, S.; Seo, S.-K.; Choi, J.S.; Jung, W.-K.; Choi, I.-W. Benzyl Isothiocyanate Attenuates Inflammasome Activation in Pseudomonas aeruginosa LPS-Stimulated THP-1 Cells and Exerts Regulation through the MAPKs/NF-κB Pathway. Int. J. Mol. Sci. 2022, 23, 1228. https://doi.org/10.3390/ijms23031228

AMA Style

Park WS, Lee J, Na G, Park S, Seo S-K, Choi JS, Jung W-K, Choi I-W. Benzyl Isothiocyanate Attenuates Inflammasome Activation in Pseudomonas aeruginosa LPS-Stimulated THP-1 Cells and Exerts Regulation through the MAPKs/NF-κB Pathway. International Journal of Molecular Sciences. 2022; 23(3):1228. https://doi.org/10.3390/ijms23031228

Chicago/Turabian Style

Park, Won S., Jeonghan Lee, Giyoun Na, SaeGwang Park, Su-Kil Seo, Jung S. Choi, Won-Kyo Jung, and Il-Whan Choi. 2022. "Benzyl Isothiocyanate Attenuates Inflammasome Activation in Pseudomonas aeruginosa LPS-Stimulated THP-1 Cells and Exerts Regulation through the MAPKs/NF-κB Pathway" International Journal of Molecular Sciences 23, no. 3: 1228. https://doi.org/10.3390/ijms23031228

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Benzyl Isothiocyanate Attenuates Inflammasome Activation in Pseudomonas aeruginosa LPS-Stimulated THP-1 Cells and Exerts Regulation through the MAPKs/NF-κB Pathway

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