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Article

Benzyl Isothiocyanate Attenuates Inflammasome Activation in Pseudomonas aeruginosa LPS-Stimulated THP-1 Cells and Exerts Regulation through the MAPKs/NF-κB Pathway

1
Department of Physiology, Kangwon National University School of Medicine, Chuncheon 24341, Korea
2
Department of Anesthesiology and Pain Medicine, Busan Paik Hospital, Inje University, Busan 47392, Korea
3
Department of Microbiology and Immunology, College of Medicine, Inje University, Busan 47392, Korea
4
Department of Internal Medicine, Busan Paik Hospital, Inje University, Busan 47392, Korea
5
Department of Biomedical Engineering, Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 48513, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Giuseppe Pugliese
Int. J. Mol. Sci. 2022, 23(3), 1228; https://doi.org/10.3390/ijms23031228
Received: 24 December 2021 / Revised: 17 January 2022 / Accepted: 20 January 2022 / Published: 22 January 2022
(This article belongs to the Section Molecular Immunology)
Inflammasomes are a group of intracellular multiprotein platforms that play important roles in immune systems. Benzyl isothiocyanate (BITC) is a constituent of cruciferous plants and has been confirmed to exhibit various biological activities. The modulatory effects of BITC on inflammasome-mediated interleukin (IL)-1β expression and its regulatory mechanisms in Pseudomonas aeruginosa (P. aeruginosa) LPS/ATP-stimulated THP-1 cells was investigated. Monocytic THP-1 cells were treated with phorbol myristate acetate (PMA) to induce differentiation into macrophages. Enzyme-linked immunosorbent assays (ELISA) were performed to measure the levels of IL-1β produced in P. aeruginosa LPS/ATP-exposed THP-1 cells. Western blotting was performed to examine the BITC modulatory mechanisms in inflammasome-mediated signaling pathways. BITC inhibited IL-1β production in P. aeruginosa LPS/ATP-induced THP-1 cells. BITC also inhibited activation of leucine-rich repeat protein-3 (NLRP3) and caspase-1 in P. aeruginosa LPS/ATP-induced THP-1 cells. Furthermore, we show that mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) activation in P. aeruginosa LPS was attenuated by BITC. These BITC-mediated modulatory effects on IL-1β production may have therapeutic potential for inflammasome-mediated disorders such as a nasal polyp. View Full-Text
Keywords: inflammasomes; benzyl isothiocyanate; interleukin-1β; Pseudomonas aeruginosa; THP-1 cells; nuclear factor-κB inflammasomes; benzyl isothiocyanate; interleukin-1β; Pseudomonas aeruginosa; THP-1 cells; nuclear factor-κB
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MDPI and ACS Style

Park, W.S.; Lee, J.; Na, G.; Park, S.; Seo, S.-K.; Choi, J.S.; Jung, W.-K.; Choi, I.-W. Benzyl Isothiocyanate Attenuates Inflammasome Activation in Pseudomonas aeruginosa LPS-Stimulated THP-1 Cells and Exerts Regulation through the MAPKs/NF-κB Pathway. Int. J. Mol. Sci. 2022, 23, 1228. https://doi.org/10.3390/ijms23031228

AMA Style

Park WS, Lee J, Na G, Park S, Seo S-K, Choi JS, Jung W-K, Choi I-W. Benzyl Isothiocyanate Attenuates Inflammasome Activation in Pseudomonas aeruginosa LPS-Stimulated THP-1 Cells and Exerts Regulation through the MAPKs/NF-κB Pathway. International Journal of Molecular Sciences. 2022; 23(3):1228. https://doi.org/10.3390/ijms23031228

Chicago/Turabian Style

Park, Won S., Jeonghan Lee, Giyoun Na, SaeGwang Park, Su-Kil Seo, Jung S. Choi, Won-Kyo Jung, and Il-Whan Choi. 2022. "Benzyl Isothiocyanate Attenuates Inflammasome Activation in Pseudomonas aeruginosa LPS-Stimulated THP-1 Cells and Exerts Regulation through the MAPKs/NF-κB Pathway" International Journal of Molecular Sciences 23, no. 3: 1228. https://doi.org/10.3390/ijms23031228

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