Reduced Elastin Fibers and Melanocyte Loss in Vitiliginous Skin Are Restored after Repigmentation by Phototherapy and/or Autologous Minigraft Transplantation
Abstract
:1. Introduction
2. Results
2.1. Repigmentation of Lesional Skin after Phototherapy and/or mMG
2.2. Melanocytes and Melanoblasts in Nonlesional, Lesional, and Repigmented Skin
2.3. Collagen and Elastin Fibers in Nonlesional, Lesional, and Repigmented Skin
2.4. Differences between Nonlesional and Repigmented Skin in the Number of Epidermal Melanocytes and Melanoblasts
2.5. Differences between Single and Double Phototherapies in the Recovery of Epidermal Melanocytes/Melanocytes and Elastin Fibers in the Repigmented Skin
2.6. Differences between Phototherapy Alone and Phototherapy plus mMG in the Recovery of Epidermal Melanocytes/Melanocytes and Elastin Fibers in the Repigmented Skin
2.7. Gender Differences in the Recovery of Epidermal Melanocytes/Melanocytes and Elastin Fibers in the Repigmented Skin after Phototherapy and/or Phototherapy plus mMG
2.8. Age Differences in the Recovery of Epidermal Melanocytes/Melanocytes and Elastin Fibers in the Repigmented Skin after Phototherapy and Phototherapy plus mMG
3. Discussion
4. Materials and Methods
4.1. Exposures of MEL and NB-UVB
4.2. Skin Transplantation (mMG)
4.3. Collection of Skin Samples
4.4. Histochemistry
4.5. Statistics
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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No. | Gender (Age) | Skin | MEL/NB (No. of Sess) | MEL/NB Month | Dose J/cm2 | mMG Month | No. of M | No. of Mb | Collagen | Elastin | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Non | Les | Repig | Non | Les | Repig | Les | Repig | Les | Repig | |||||||
1. | M (15) | Belly | NB (50) | 12 | 38.3 | No | 110.9 | 0 | 105.8 | 104.3 | 0 | 106.3 | → | → | ↓↓↓ | ↑↑↑ |
2. | M (31) | Chin | NB (70) | 10 | 51.3 | No | 134.8 | 0 | 120.4 | 117.4 | 0 | 82.9 | → | → | ↓↓ | ↑↑↑ |
3. | F (11) | Neck | MEL (8) + NB (8) | 4 | 4.1 | 4 | 127.5 | 0 | 177.9 | 103.8 | 0 | 103.9 | ↓ | ↑ | ↓↓↓ | ↑↑↑ |
4. | M (71) | Neck | MEL (1) + NB (6) | 1 | 10.1 | 1 | 53.7 | 0 | 82.3 | 95.0 | 0 | 94.0 | → | → | ↓↓↓ | ↑↑↑ |
5. | F (45) | Neck | MEL (5) + NB (6) | 0.7 | 4.5 | 0.7 | 87.5 | 0 | 91.6 | 90.0 | 0 | 71.1 | → | → | ↓↓↓ | ↑↑↑ |
6. | M (15) | Neck | MEL (3) + NB (1) | 2 | 1.1 | 2 | 111.3 | 0 | 167.8 | 98.9 | 0 | 150.4 | → | → | ↓↓ | ↑↑↑ |
7. | M (6) | Eyelid | MEL (27) + NB (3) | 5 | 8.0 | 5 | 107.8 | 0 | 108.3 | 99.9 | 0 | 126.9 | → | → | ↓ | ↑↑ |
8. | M (10) | Head | MEL (35) + NB (31) | 10 | 50.1 | 10 | 125.2 | 0 | 299.5 | 100.1 | 0 | 283.5 | → | → | ↓↓ | ↑↑ |
9. | F (14) | Eyelid | MEL (4) | 1 | 0.3 | No | 130.5 | 0 | 85.7 | 102.7 | 0 | 96.4 | → | → | ↓ | ↑↑ |
10. | F (13) | Face | MEL (12) + NB (13) | 3 | 7.6 | No | 93.4 | 0 | 126.7 | 101.2 | 0 | 128.6 | → | → | ↓ | ↑↑ |
11. | F (41) | Eyelid | MEL (2) + NB (38) | 7 | 25.5 | 7 | 109.9 | 0 | 174.1 | 113.8 | 0 | 108.1 | → | → | ↓↓↓ | ↑↑↑ |
12. | F (71) | Arm | NB (51) | 24 | 52.4 | No | 98.5 | 0 | 107.6 | 103.5 | 0 | 97.9 | ↓ | ↑ | ↓↓↓ | ↑↑↑ |
13. | F (77) | Hand | NB (151) | 12 | 64.1 | No | 150.5 | 0 | 138.6 | 107.3 | 0 | 99.5 | → | → | ↓↓↓ | ↑↑↑ |
14. | M (65) | Cheek | MEL (12) + NB (12) | 2 | 8.2 | 2 | 120.8 | 0 | 88.4 | 106.5 | 0 | 89.9 | → | → | ↓↓↓ | ↑↑↑ |
Ave | 34.6 ± 6.8 | 39.2 ± 10.0 | 6.7 ± 1.7 | 23.3 ± 5.9 | 111.6 ± 6.1 | 133.9 ± 14.9 | 103.2 ± 1.8 | 117.1 ± 13.4 |
No. of Melanocytes/0.1 mm2 | No. of Melanoblasts/0.1 mm2 | |||
---|---|---|---|---|
(A) | Single MEL/NB vs Double MEL/NB | |||
Single | Double | Single | Double | |
(n = 5) | (n = 9) | (n = 5) | (n = 9) | |
111.6 ± 7.8 | 146.3 ± 21.7 | 96.6 ± 3.4 | 128.5 ± 19.7 | |
(B) | MEL/NB alone vsMEL/NB + mMG | |||
MEL/NB | MEL/NB + mMG | MEL/NB | MEL/NB + mMG | |
(n = 6) | (n = 8) | (n = 6) | (n = 8) | |
114.1 ± 6.9 | 148.7 ± 24.2 | 101.9 ± 5.6 | 128.5 ± 22.2 | |
(C) | Gender | |||
Female | Male | Female | Male | |
(n = 7) | (n = 7) | (n = 7) | (n = 7) | |
128.9 ± 13.0 | 138.9 ± 26.6 | 100.8 ± 6.0 | 133.4 ± 24.6 | |
(D) | Age | |||
Young | Old | Young | Old | |
(n = 7) | (n = 7) | (n = 7) | (n = 7) | |
153.1 ± 25.5 | 114.7 ± 11.5 | 142.3 ± 22.7 * | 91.9 ± 4.2 * |
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Hirobe, T.; Enami, H. Reduced Elastin Fibers and Melanocyte Loss in Vitiliginous Skin Are Restored after Repigmentation by Phototherapy and/or Autologous Minigraft Transplantation. Int. J. Mol. Sci. 2022, 23, 15361. https://doi.org/10.3390/ijms232315361
Hirobe T, Enami H. Reduced Elastin Fibers and Melanocyte Loss in Vitiliginous Skin Are Restored after Repigmentation by Phototherapy and/or Autologous Minigraft Transplantation. International Journal of Molecular Sciences. 2022; 23(23):15361. https://doi.org/10.3390/ijms232315361
Chicago/Turabian StyleHirobe, Tomohisa, and Hisao Enami. 2022. "Reduced Elastin Fibers and Melanocyte Loss in Vitiliginous Skin Are Restored after Repigmentation by Phototherapy and/or Autologous Minigraft Transplantation" International Journal of Molecular Sciences 23, no. 23: 15361. https://doi.org/10.3390/ijms232315361