Fluoxetine Enhances Synaptic Vesicle Trafficking and Energy Metabolism in the Hippocampus of Socially Isolated Rats
Abstract
:1. Introduction
2. Results
2.1. Differential Proteomics Analysis
2.2. Analysis of Protein–Protein Network Interaction by STRING
2.3. Western Blot Analysis
3. Discussion
Limitation
4. Materials and Methods
4.1. Animals
4.2. Study Design
4.3. Fluoxetine-Hydrochloride Administration
4.4. Preparation of Synaptosomal Fractions from the Rat Hippocampus
4.5. Electrophoresis, In-Gel Digestion and LC-MS/MS
4.6. Western Blot Validation of the Proteomic Results
4.7. Bioinformatics and Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Accession No | Gene | Ratio | Matched Peptides | Unique Peptides |
---|---|---|---|---|---|
ATP synthase subunit alpha, mitochondrial | P15999 | Atp5f1a | 0.80 | 11 | 10 |
Malic enzyme | A0A0G2K4C6 | Me3 | 0.79 | 2 | 2 |
RAB5B, member RAS oncogene family | A1L1J8 | Rab5b | 0.79 | 3 | 2 |
Enolase 1, (Alpha) | Q5EB49 | Eno1 | 0.78 | 18 | 16 |
Annexin | O70371 | N/A | 0.77 | 15 | 15 |
Septin 5 | D3ZDH8 | Sep5 | 0.77 | 5 | 5 |
Malate dehydrogenase, cytoplasmic | O88989 | Mdh1 | 0.76 | 13 | 13 |
F-actin-capping protein subunit alpha-2 | Q3T1K5 | Capza2 | 0.76 | 3 | 3 |
Fructose-bisphosphate aldolase A | P05065 | Aldoa | 0.75 | 22 | 18 |
NAD-dependent protein deacetylase sirtuin-2 | Q5RJQ4 | Sirt2 | 0.75 | 3 | 3 |
Pyruvate dehydrogenase E1 component subunit beta | A0A0G2KAM3 | Pdhb | 0.74 | 18 | 16 |
Cofilin-1 | P45592 | Cfl1 | 0.74 | 2 | 2 |
Annexin | Q5U362 | Anxa4 | 0.72 | 6 | 6 |
Creatine kinase B-type | P07335 | Ckb | 0.70 | 27 | 25 |
Annexin | Q6IMZ3 | Anxa6 | 0.70 | 17 | 15 |
Malate dehydrogenase, mitochondrial | P04636 | Mdh2 | 0.68 | 31 | 30 |
Peroxiredoxin 3 | G3V7I0 | Prdx3 | 0.65 | 3 | 3 |
Glutathione S-transferase P | P04906 | Gstp1 | 0.63 | 4 | 4 |
Annexin A3 | P14669 | Anxa3 | 0.62 | 11 | 11 |
Cytochrome c oxidase subunit | D3ZD09 | Cox6b1 | 0.50 | 2 | 2 |
Name | Accession No | Gene | Ratio | Matched Peptides | Unique Peptides |
---|---|---|---|---|---|
ATP synthase protein 8 | Q5UAJ5 | ATP8 | 5.77 | 2 | 2 |
Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a | Q5XI34 | Ppp2r1a | 2.25 | 2 | 2 |
Tropomyosin alpha-3 chain | Q63610 | Tpm3 | 2.11 | 5 | 3 |
10 kDa heat shock protein, mitochondrial | P26772 | Hspe1 | 2.10 | 8 | 8 |
Calcium/calmodulin-dependent protein kinase type II subunit alpha | P11275 | Camk2a | 1.72 | 5 | 3 |
Polyubiquitin | Q63654 | UBC | 1.68 | 6 | 2 |
Isoform Non-brain of Clathrin light chain | P08082-2 | Cltb | 1.64 | 2 | 2 |
Elongation factor 1-gamma | Q68FR6 | Eef1g | 1.52 | 3 | 3 |
Name | Accession No | Gene | Ratio | Matched Peptides | Unique Peptides |
---|---|---|---|---|---|
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit | A0A0G2JTA1 | Ppp2r5e | 2.31 | 3 | 2 |
Tubulin beta-2A chain | P85108 | Tubb2a | 2.27 | 15 | 5 |
ATP synthase subunit beta | G3V6D3 | Atp5f1b | 2.21 | 26 | 24 |
V-type proton ATPase subunit H | A0A0G2K9J2 | Atp6v1h | 2.20 | 6 | 5 |
AP-2 complex subunit beta | P62944 | Ap2b1 | 2.20 | 11 | 5 |
T-complex protein 1 subunit beta | Q5XIM9 | Cct2 | 2.17 | 3 | 3 |
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform | P63329 | Ppp3ca | 2.15 | 8 | 6 |
Alpha-actinin-1 | Q9Z1P2 | Actn1 | 2.03 | 14 | 8 |
ATP synthase subunit alpha, mitochondrial | P15999 | Atp5f1a | 2.03 | 14 | 14 |
26S proteasome regulatory subunit 7 | Q63347 | Psmc2 | 2.00 | 2 | 2 |
Beta-soluble NSF attachment protein | F8WFM2 | Napb | 1.97 | 7 | 6 |
Neurochondrin | O35095 | Ncdn | 1.92 | 8 | 6 |
Long-chain-fatty-acid--CoA ligase ACSBG1 | Q924N5 | Acsbg1 | 1.89 | 5 | 3 |
Dynamin-1 | P21575 | Dnm1 | 1.89 | 32 | 29 |
Clathrin heavy chain 1 | P11442 | Cltc | 1.88 | 75 | 71 |
Clathrin heavy chain | F1M779 | Cltc | 1.88 | 75 | 72 |
Phosphoglucomutase 1 | Q499Q4 | Pgm1 | 1.87 | 5 | 5 |
4-aminobutyrate aminotransferase, mitochondrial | P50554 | Abat | 1.87 | 17 | 16 |
Heat shock 70kDa protein 12A (Predicted), isoform CRA_a | D3ZC55 | Hspa12a | 1.86 | 3 | 3 |
Kynurenine--oxoglutarate transaminase 3 | Q58FK9 | Kyat3 | 1.86 | 3 | 3 |
Adducin 1 (Alpha), isoform CRA_b | A0A0G2JSM7 | Add1 | 1.86 | 5 | 4 |
Synaptotagmin-1 | P21707 | Syt1 | 1.85 | 4 | 3 |
Dihydropyrimidinase-related protein | Q9JMG8 | N/A | 1.83 | 3 | 3 |
Glutaminase kidney isoform, mitochondrial | P13264 | Gls | 1.83 | 14 | 10 |
Dmx-like 2 | F1M3W5 | Dmxl2 | 1.82 | 2 | 2 |
Phosphodiesterase | F8WFW5 | Pde2a | 1.82 | 2 | 2 |
Fumarylacetoacetate hydrolase domain-containing protein 2 | B2RYW9 | Fahd2 | 1.79 | 5 | 5 |
Polyubiquitin-C | F1LML2 | Ubc | 1.79 | 7 | 6 |
Guanine deaminase | Q9JKB7 | Gda | 1.79 | 11 | 11 |
Copine 6 protein | H1UBM5 | Cpne6 | 1.78 | 6 | 5 |
Heat shock protein 105 kDa | Q66HA8 | Hsph1 | 1.78 | 12 | 8 |
Aspartate aminotransferase, mitochondrial | P00507 | Got2 | 1.76 | 5 | 2 |
AP-2 complex subunit alpha | D3ZUY8 | Ap2a1 | 1.76 | 14 | 7 |
Endophilin-B2 | Q5PPJ9 | Sh3glb2 | 1.72 | 3 | 2 |
Eukaryotic initiation factor 4A-II | Q5RKI1 | Eif4a2 | 1.72 | 4 | 4 |
Chaperonin containing Tcp1, subunit 6A (Zeta 1) | Q3MHS9 | Cct6a | 1.70 | 3 | 2 |
Gamma-enolase | P07323 | Eno2 | 1.69 | 10 | 7 |
2-oxoglutarate dehydrogenase, mitochondrial | Q5XI78 | Ogdh | 1.69 | 12 | 8 |
Endophilin-A1 | O35179 | Sh3gl2 | 1.69 | 11 | 10 |
Rab GDP dissociation inhibitor alpha | P50398 | Gdi1 | 1.67 | 29 | 21 |
Adenylyl cyclase-associated protein 2 | P52481 | Cap2 | 1.67 | 7 | 5 |
Aldehyde dehydrogenase X, mitochondrial | G3V7I5 | Aldh1b1 | 1.66 | 7 | 5 |
Oxidation resistance protein 1 | A0A0G2K7Y2 | Oxr1 | 1.64 | 6 | 5 |
Protein NDRG2 | Q8VBU2 | Ndrg2 | 1.64 | 9 | 9 |
Spectrin beta chain | A0A0G2K8W9 | Sptbn1 | 1.64 | 23 | 19 |
Amine oxidase | B2GV33 | Maoa | 1.63 | 3 | 3 |
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 | P10687 | Plcb1 | 1.62 | 2 | 2 |
ATPase, H+ transporting, V1 subunit E isoform 1, isoform CRA_a | G3V7L8 | Atp6v1e1 | 1.62 | 9 | 8 |
Endoplasmic reticulum chaperone BiP | P06761 | Hspa5 | 1.62 | 13 | 10 |
Pyruvate kinase PKM | P11980 | Pkm | 1.61 | 34 | 4 |
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial | Q5XIH3 | Ndufv1 | 1.60 | 4 | 4 |
Pck2 protein | B2RYG2 | Pck2 | 1.60 | 12 | 12 |
Ubiquitin-like modifier-activating enzyme 1 | Q5U300 | Uba1 | 1.60 | 16 | 14 |
14-3-3 protein epsilon | P62260 | Ywhae | 1.60 | 18 | 15 |
Heat shock 70kDa protein 4-like (Predicted), isoform CRA_b | B4F772 | Hspa4l | 1.59 | 9 | 7 |
Dihydropyrimidinase-related protein 3 | Q62952 | Dpysl3 | 1.58 | 10 | 5 |
Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex | A0A0G2JZH8 | Pdhx | 1.58 | 9 | 7 |
Alpha-1,4 glucan phosphorylase | G3V6Y6 | Pygb | 1.58 | 12 | 8 |
Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a | Q5XI34 | Ppp2r1a | 1.58 | 10 | 10 |
D-3-phosphoglycerate dehydrogenase | O08651 | Phgdh | 1.57 | 4 | 3 |
N-ethylmaleimide sensitive fusion protein, isoform CRA_b | F1LQ81 | Nsf | 1.57 | 22 | 21 |
Heat shock cognate 71 kDa protein | P63018 | Hspa8 | 1.57 | 44 | 36 |
Heat shock 70 kDa protein 4 | F1LRV4 | Hspa4 | 1.56 | 16 | 12 |
Heat shock protein HSP 90-alpha | P82995 | Hsp90aa1 | 1.56 | 27 | 20 |
Dynein light chain 1, cytoplasmic | P63170 | Dynll1 | 1.55 | 2 | 2 |
Elongation factor 1-gamma | Q68FR6 | Eef1g | 1.55 | 2 | 2 |
Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial | Q02253 | Aldh6a1 | 1.55 | 3 | 3 |
Tenascin R, isoform CRA_b | A0A096MJE6 | Tnr | 1.55 | 12 | 10 |
ATPase H+-transporting V1 subunit A | D4A133 | Atp6v1a | 1.55 | 39 | 38 |
5′-nucleotidase domain-containing 3 | D3ZAI6 | Nt5dc3 | 1.54 | 8 | 7 |
Aminopeptidase | F1M9V7 | Npepps | 1.54 | 8 | 7 |
L-lactate dehydrogenase B chain | P42123 | Ldhb | 1.54 | 15 | 12 |
60 kDa heat shock protein, mitochondrial | P63039 | Hspd1 | 1.54 | 39 | 36 |
Phosphatidylethanolamine-binding protein 1 | P31044 | Pebp1 | 1.53 | 3 | 3 |
Malate dehydrogenase, cytoplasmic | O88989 | Mdh1 | 1.53 | 12 | 12 |
Intercellular adhesion molecule 5 | D4A435 | Icam5 | 1.52 | 4 | 4 |
Fructose-bisphosphate aldolase A | P05065 | Aldoa | 1.52 | 5 | 4 |
Ras-related protein Rab-3A | P63012 | Rab3a | 1.52 | 11 | 10 |
Spectrin alpha chain, nonerythrocytic 1 | A0A0G2JZ69 | Sptan1 | 1.52 | 44 | 35 |
Receptor-type tyrosine-protein phosphatase zeta | F1LMY3 | Ptprz1 | 1.51 | 2 | 2 |
Opioid-binding protein/cell adhesion molecule | P32736 | Opcml | 1.51 | 4 | 3 |
Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial | B2GV06 | Oxct1 | 1.51 | 16 | 15 |
Enolase 1, (Alpha) | Q5EB49 | Eno1 | 1.51 | 22 | 19 |
Dihydropyrimidinase-related protein 2 | P47942 | Dpysl2 | 1.51 | 49 | 41 |
Profilin-2 | Q9EPC6 | Pfn2 | 1.50 | 2 | 2 |
Microtubule-associated protein 6 | Q63560 | Map6 | 0.79 | 3 | 3 |
10 kDa heat shock protein, mitochondrial | P26772 | Hspe1 | 0.79 | 6 | 6 |
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 | D3ZS58 | Ndufa2 | 0.73 | 2 | 2 |
Isoform Excitatory amino acid transporter 2 | P31596-2 | Slc1a2 | 0.73 | 7 | 7 |
Myelin proteolipid protein | P60203 | Plp1 | 0.69 | 6 | 5 |
ATP synthase subunit e, mitochondrial | P29419 | Atp5me | 0.36 | 4 | 3 |
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Filipović, D.; Costina, V.; Findeisen, P.; Inta, D. Fluoxetine Enhances Synaptic Vesicle Trafficking and Energy Metabolism in the Hippocampus of Socially Isolated Rats. Int. J. Mol. Sci. 2022, 23, 15351. https://doi.org/10.3390/ijms232315351
Filipović D, Costina V, Findeisen P, Inta D. Fluoxetine Enhances Synaptic Vesicle Trafficking and Energy Metabolism in the Hippocampus of Socially Isolated Rats. International Journal of Molecular Sciences. 2022; 23(23):15351. https://doi.org/10.3390/ijms232315351
Chicago/Turabian StyleFilipović, Dragana, Victor Costina, Peter Findeisen, and Dragos Inta. 2022. "Fluoxetine Enhances Synaptic Vesicle Trafficking and Energy Metabolism in the Hippocampus of Socially Isolated Rats" International Journal of Molecular Sciences 23, no. 23: 15351. https://doi.org/10.3390/ijms232315351