Nutrition Alters the Stiffness of Adipose Tissue and Cell Signaling
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Histology and Immunofluorecence Staining and Analysis
4.3. Picrosirius Red Staining for Collagen Fibers
4.4. Transmission Electron Microscopy (TEM)
4.5. Tissue Extracellular Matrix (ECM) and Decellularization
4.6. Rheological Measurements
4.7. Whole-Mount immunofluorescence staining
4.8. Biochemistry and Protein Identification Western Blot (WB)
4.9. RNA Isolation and qPCR
Gene | Forward Primer | Reverse Primer |
Actin | CATCGTGGGCCGCCCTAGGCACCA | CGGTTGGCCTTAGGGTTCAGGGGG |
Rage | AACACAGCCCCCATCCAA | GCTCA CCAACAGCTGAATG |
Glut4 | TTCACGTTGGTCTCGGTGCT | TAGCTCATGGCTGGAACCCG |
4.10. Statistical Analysis
4.11. Schematic Illustrations
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Naftaly, A.; Kislev, N.; Izgilov, R.; Adler, R.; Silber, M.; Shalgi, R.; Benayahu, D. Nutrition Alters the Stiffness of Adipose Tissue and Cell Signaling. Int. J. Mol. Sci. 2022, 23, 15237. https://doi.org/10.3390/ijms232315237
Naftaly A, Kislev N, Izgilov R, Adler R, Silber M, Shalgi R, Benayahu D. Nutrition Alters the Stiffness of Adipose Tissue and Cell Signaling. International Journal of Molecular Sciences. 2022; 23(23):15237. https://doi.org/10.3390/ijms232315237
Chicago/Turabian StyleNaftaly, Alex, Nadav Kislev, Roza Izgilov, Raizel Adler, Michal Silber, Ruth Shalgi, and Dafna Benayahu. 2022. "Nutrition Alters the Stiffness of Adipose Tissue and Cell Signaling" International Journal of Molecular Sciences 23, no. 23: 15237. https://doi.org/10.3390/ijms232315237