Secretome Analysis of the Plant Biostimulant Bacteria Strains Bacillus subtilis (EB2004S) and Lactobacillus helveticus (EL2006H) in Response to pH Changes
Abstract
:1. Introduction
2. Results
2.1. Proteome Analysis of L. helveticus (EL2006H) CFS
2.2. Groups of Identified Proteins from L. helveticus (EL2006H) CFS and Their Functional Annotation
2.3. Identified Proteins from L. helveticus (EL2006H) CFS
2.4. Proteome Analysis of B. subtilis (EB2004S) CFS
2.5. Groups of Identified Proteins from B. subtilis (EB2004S) CFS and Their Functional Annotation
2.6. Identified Proteins with Differential Identities from B. subtilis (EB2004S) CFS
3. Discussion
4. Materials and Methods
4.1. Microbial Strain Growth Conditions and Media
Proteomics Study from the CFSs
4.2. Protein Profiling
4.3. Protein Identification Confidence Level
4.4. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameter | B. subtilis | L. helveticus |
---|---|---|
Genome size (bp) | 4,214,630 [23,32] | 21,000 [30] |
Optimum growth pH range | 7.0 [18,29] | 5.5 to 5.8 [18,30] |
Proteins protein-coding genes | 4610 [33] | 2462 [34] |
Enzyme Code Classes | B. subtilis (EB2004S) | L. helveticus (EL2006H) | |||
---|---|---|---|---|---|
pH 7 | pH 5 | pH 8 | pH 7 | pH 5 | |
Hydrolases | 591 | 34 (↓94%) | 644 (↑9%) | 431 | 118 (↓73%) |
Isomerases | 92 | 2 (↓98%) | 123 (↑33%) | 66.3 | 13.7 (↓79%) |
Ligases | 155 | 0 (↓100%) | 188 (↑21%) | 103.3 | 1.7 (↓98%) |
Lyases | 134 | 0 (↓100%) | 199 (↑49%) | 44.7 | 6.7 (↓85%) |
Oxidoreductases | 392 | 20 (↓95%) | 490 (↑25%) | 72 | 0 (↓100%) |
Transferases | 457 | 6 (↓98 %) | 584 (↑28%) | 194.7 | 3 (↓99%) |
Translocases | 95 | 0 (↓100%) | 119 (↑26%) | 31.3 | 6.7 (↓79%) |
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Msimbira, L.A.; Subramanian, S.; Naamala, J.; Antar, M.; Smith, D.L. Secretome Analysis of the Plant Biostimulant Bacteria Strains Bacillus subtilis (EB2004S) and Lactobacillus helveticus (EL2006H) in Response to pH Changes. Int. J. Mol. Sci. 2022, 23, 15144. https://doi.org/10.3390/ijms232315144
Msimbira LA, Subramanian S, Naamala J, Antar M, Smith DL. Secretome Analysis of the Plant Biostimulant Bacteria Strains Bacillus subtilis (EB2004S) and Lactobacillus helveticus (EL2006H) in Response to pH Changes. International Journal of Molecular Sciences. 2022; 23(23):15144. https://doi.org/10.3390/ijms232315144
Chicago/Turabian StyleMsimbira, Levini A., Sowmyalakshmi Subramanian, Judith Naamala, Mohammed Antar, and Donald L. Smith. 2022. "Secretome Analysis of the Plant Biostimulant Bacteria Strains Bacillus subtilis (EB2004S) and Lactobacillus helveticus (EL2006H) in Response to pH Changes" International Journal of Molecular Sciences 23, no. 23: 15144. https://doi.org/10.3390/ijms232315144