Rapid Detection of Brettanomyces bruxellensis in Wine by Polychromatic Flow Cytometry
Abstract
:1. Introduction
2. Results
2.1. Specificity of the Flow Cytometry Measurements
2.2. Sensitivity of B. bruxellensis Flow Cytometry Measurements
2.3. Flow Cytometry Analyses of S. cerevisiae and B. bruxellensis Mixed Cultures
2.4. Flow Cytometry Identification and Count of B. bruxellensis in Wine Samples
3. Discussion
4. Materials and Methods
4.1. B. bruxellensis Cell Cultures
4.2. Wine Samples
4.3. Flow Cytometry Analyses
4.4. B. bruxellensis Counts
4.5. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wine Sample | Inoculated Cells | Concentration (Cells/mL) |
---|---|---|
S1 | B. bruxellensis | 8 × 105 |
S2 | B. bruxellensis | 8 × 104 |
S. cerevisiae | 8 × 103 | |
S3 | B. bruxellensis | 8 × 103 |
S. cerevisiae | 8 × 103 | |
Pichia spp. | 8 × 103 |
Wine Sample | Inoculated Cells | Concentration (Cells/mL) |
---|---|---|
SW5 | B. bruxellensis | (6.0 ± 0.3) × 103 |
SW6 | B. bruxellensis | (3.9 ± 0.1) × 104 |
O. oeni | (3.3 ± 0.3) × 103 | |
SW7 | B. bruxellensis | (9.4 ± 0.5) × 102 |
Pichia spp. | (2.6 ± 0.7) × 102 |
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De Bellis, D.; Di Stefano, A.; Simeone, P.; Catitti, G.; Vespa, S.; Patruno, A.; Marchisio, M.; Mari, E.; Granchi, L.; Viti, C.; et al. Rapid Detection of Brettanomyces bruxellensis in Wine by Polychromatic Flow Cytometry. Int. J. Mol. Sci. 2022, 23, 15091. https://doi.org/10.3390/ijms232315091
De Bellis D, Di Stefano A, Simeone P, Catitti G, Vespa S, Patruno A, Marchisio M, Mari E, Granchi L, Viti C, et al. Rapid Detection of Brettanomyces bruxellensis in Wine by Polychromatic Flow Cytometry. International Journal of Molecular Sciences. 2022; 23(23):15091. https://doi.org/10.3390/ijms232315091
Chicago/Turabian StyleDe Bellis, Domenico, Alessio Di Stefano, Pasquale Simeone, Giulia Catitti, Simone Vespa, Antonia Patruno, Marco Marchisio, Eleonora Mari, Lisa Granchi, Carlo Viti, and et al. 2022. "Rapid Detection of Brettanomyces bruxellensis in Wine by Polychromatic Flow Cytometry" International Journal of Molecular Sciences 23, no. 23: 15091. https://doi.org/10.3390/ijms232315091