The Upregulation of Regenerative Activity for Extracellular Vesicles with Melatonin Modulation in Chemically Defined Media
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of hUCMSC-Derived EVs Depending on Culture Conditions
2.2. The Expectations for the Functionality of EVs through Comparative Analysis on Internal miRNA of EVs
2.3. Angiogenesis and Wound-Healing Effects of EVs
2.4. Anti-Inflammation Effects of EVs
2.5. Anti-Oxidative and Anti-Apoptosis Effects of EVs
3. Materials and Methods
3.1. Cell Culture
3.2. Isolation and Characterization of Extracellular Vesicles (EVs)
3.3. Western Blot Analysis
3.4. Quantification of the miRNA in EVs
3.5. Bioinformatics Analysis of the Internal miRNA in EVs
3.6. Tube Formation Assay
3.7. Cell Migration Assay
3.8. Immunocytochemistry (ICC)
3.9. RT-qPCR for Pro-Inflammatory Factors
3.10. Cellular ROS Assay with 2′,7′-Dichlorofluorescin Diacetate (DCF-DA)
3.11. Apoptosis Assay
3.12. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Kim, J.Y.; Rhim, W.-K.; Woo, J.; Cha, S.-G.; Park, C.G.; Han, D.K. The Upregulation of Regenerative Activity for Extracellular Vesicles with Melatonin Modulation in Chemically Defined Media. Int. J. Mol. Sci. 2022, 23, 15089. https://doi.org/10.3390/ijms232315089
Kim JY, Rhim W-K, Woo J, Cha S-G, Park CG, Han DK. The Upregulation of Regenerative Activity for Extracellular Vesicles with Melatonin Modulation in Chemically Defined Media. International Journal of Molecular Sciences. 2022; 23(23):15089. https://doi.org/10.3390/ijms232315089
Chicago/Turabian StyleKim, Jun Yong, Won-Kyu Rhim, Jiwon Woo, Seung-Gyu Cha, Chun Gwon Park, and Dong Keun Han. 2022. "The Upregulation of Regenerative Activity for Extracellular Vesicles with Melatonin Modulation in Chemically Defined Media" International Journal of Molecular Sciences 23, no. 23: 15089. https://doi.org/10.3390/ijms232315089