Down-Regulation of Neogenin Decreases Proliferation and Differentiation of Spermatogonia during the Early Phase of Spermatogenesis
Abstract
:1. Introduction
2. Results
2.1. Neogenin Expression Varies during Spermatogenesis
2.2. Neogenin Down-Regulation Hampers Differentiation of Primary Spermatocytes into Secondary Spermatocytes
2.3. Expression Profiles of Spermatogonial Stem Cell Markers in Neogenin-cKO Testes
2.4. Apoptosis in Neogenin-cKO Testes
3. Discussion
4. Materials and Methods
4.1. Preparation of Mouse Testes
4.2. Histochemical Examination of Testes
4.3. PCR for Gene Expression Analysis
4.4. Western Blotting for Protein Expression Analysis
4.5. cKO of Neogenin in the Testis
4.6. Analysis of Apoptosis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Park, J.W.; Kim, Y.J.; Lee, S.J.; Ko, J.J.; Kim, D.K.; Lee, J.H. Down-Regulation of Neogenin Decreases Proliferation and Differentiation of Spermatogonia during the Early Phase of Spermatogenesis. Int. J. Mol. Sci. 2022, 23, 14761. https://doi.org/10.3390/ijms232314761
Park JW, Kim YJ, Lee SJ, Ko JJ, Kim DK, Lee JH. Down-Regulation of Neogenin Decreases Proliferation and Differentiation of Spermatogonia during the Early Phase of Spermatogenesis. International Journal of Molecular Sciences. 2022; 23(23):14761. https://doi.org/10.3390/ijms232314761
Chicago/Turabian StylePark, Jin Woo, Yu Jin Kim, Sang Jin Lee, Jung Jae Ko, Dae Keun Kim, and Jae Ho Lee. 2022. "Down-Regulation of Neogenin Decreases Proliferation and Differentiation of Spermatogonia during the Early Phase of Spermatogenesis" International Journal of Molecular Sciences 23, no. 23: 14761. https://doi.org/10.3390/ijms232314761