Mitochondrial DNA as a Candidate Marker of Multiple Organ Failure after Cardiac Surgery
Abstract
:1. Introduction
2. Results
2.1. MtDNA Levels in Patients
2.2. Features of MOF in Patients with Complications
3. Discussion
4. Material and Methods
4.1. Cohort Description
4.2. Blood Sampling
4.3. DNA Isolation and Quantitative mtDNA Analysis
4.4. Statistical Data Processing
5. Conclusions
- The level of mtDNA significantly increases in case of the development of MOF, regardless of its cause, in a small cohort of cardiac surgery patients.
- The level of mtDNA correlates with the severity of MOF on the SOFA score.
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
Abbreviations
ADQI | Acute dialysis quality initiative |
CAM-ICU | Confusion Assessment Method for Intensive Care Unit |
SIRS | Systemic Inflammatory Response Syndrome |
COX | Cytochrome oxidase |
DAMP | Danger-associated molecular patterns |
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase |
NADH | Nicotinamide adenine dinucleotide hydrogen |
PAMP | Pathogen-associated molecular patterns |
SOFA | Sequential Organ Failure Assessment |
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Parameter | Patients without Complications (Group 1, n = 30) | Patients with Complications (Group 2, n = 30) | p |
---|---|---|---|
Age, years old | 57.2 (42.0–66.0) | 59.2 (48.0–66.9) | ns |
Male, abs. (%) | 15 (50) | 15 (41.6) | ns |
Body weight index, kg/m2 | 33.4 (32.1–35.2) | 36.4 (32.0–36.7) | ns |
Comorbidity by CIRS, points | 9.6 (8.0–11.1) | 9.0 (8.9–11.5) | ns |
Aortic valve surgery, abs (%) | 5 (16.6) | 7 (23) | ns |
Mitral valve surgery, abs aбc. (%) | 6 (20) | 3 (10) | ns |
Aortic + mitral valve surgery, abs (%) | 19 (63.4) | 20 (67) | ns |
The reasons of MOF, abs (%): | |||
| Not applicable | 11 (36) 7 (23) 12 (41) | 0.0001 |
The time of artificial circulation min | 121 (89–167) | 158 (101–201) | 0.001 |
Aortic cross clump, min | 98 (90–122) | 108 (99–145) | ns |
The need for transfusion after surgery, % | 0 | 100% |
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Grigoriev, E.; Ponasenko, A.V.; Sinitskaya, A.V.; Ivkin, A.A.; Kornelyuk, R.A. Mitochondrial DNA as a Candidate Marker of Multiple Organ Failure after Cardiac Surgery. Int. J. Mol. Sci. 2022, 23, 14748. https://doi.org/10.3390/ijms232314748
Grigoriev E, Ponasenko AV, Sinitskaya AV, Ivkin AA, Kornelyuk RA. Mitochondrial DNA as a Candidate Marker of Multiple Organ Failure after Cardiac Surgery. International Journal of Molecular Sciences. 2022; 23(23):14748. https://doi.org/10.3390/ijms232314748
Chicago/Turabian StyleGrigoriev, Evgeny, Anastasia V. Ponasenko, Anna V. Sinitskaya, Artem A. Ivkin, and Roman A. Kornelyuk. 2022. "Mitochondrial DNA as a Candidate Marker of Multiple Organ Failure after Cardiac Surgery" International Journal of Molecular Sciences 23, no. 23: 14748. https://doi.org/10.3390/ijms232314748