Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro
Abstract
:1. Introduction
2. Results
2.1. TCRV Inhibits PEDV Infection In Vitro
2.2. TCRV Promotes PEDV-Induced Apoptosis In Vitro
2.3. TCRV-Induced Mitochondrial Membrane Potential (MMP) Decreased in PEDV-Infected Cells
2.4. TCRV-Induced Cytochrome C Translocation after PEDV Infection
2.5. TCRV Inhibits PEDV Infection by the Regulation of Caspase-3
3. Discussion
4. Materials and Methods
4.1. Cytotoxicity Assay
4.2. Cells and Virus
4.3. Medicine Treatment
4.4. Indirect Immunofluorescence Assay (IFA)
4.5. Western Blotting (WB) Assay
4.6. RNA Extraction and Quantitative RT-PCR(RT-qPCR)
4.7. Annexin V-PE and 7-Amino-Actinomycin (7-AAD) Double-Staining Assay
4.8. Determination of Mitochondrial Membrane Potential (MMP)
4.9. Determination of the Levels of Intracellular Reactive Oxygen Species (ROS)
4.10. Mitochondria Isolation and Cytochrome C Release Assays
4.11. Indirect Immunofluorescence Assay (IFA) to Analyze Cytochrome C Subcellular Localization
4.12. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Genes | Forward 5′-3′ | Reverse 5′-3′ |
---|---|---|
PEDV ORF3 | TAGACAAGCTTCAAATGTGAC | GTATTAAAGATAATAAGGAGCGC |
GAPDH | AGGTCGGAGTCAACGGATTT | TAGTTGAGGTCAATGAAGGG |
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Wang, X.; Liu, Y.; Li, K.; Yang, M.; Wang, Q.; Hao, Z. Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro. Int. J. Mol. Sci. 2022, 23, 14499. https://doi.org/10.3390/ijms232314499
Wang X, Liu Y, Li K, Yang M, Wang Q, Hao Z. Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro. International Journal of Molecular Sciences. 2022; 23(23):14499. https://doi.org/10.3390/ijms232314499
Chicago/Turabian StyleWang, Xue, Yi Liu, Kaiyuan Li, Ming Yang, Qingtao Wang, and Zhihui Hao. 2022. "Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro" International Journal of Molecular Sciences 23, no. 23: 14499. https://doi.org/10.3390/ijms232314499