Cytochrome P450 BsCYP99A44 and BsCYP704A177 Confer Metabolic Resistance to ALS Herbicides in Beckmannia syzigachne
Abstract
:1. Introduction
2. Results
2.1. RNA-Seq and Bioinformatics Analysis
2.2. Differential Expression Analysis and Validation of P450s
2.3. Full Sequence Cloning and Analysis of CYP99A44 and CYP704A177
2.4. Sensitivity to Mesosulfuron-Methyl and Other Herbicides of Arabidopsis Overexpressing CYP99A44 and CYP704A177
2.5. Subcellular Location of CYP99A44 and CYP704A177
3. Discussion
4. Materials and Methods
4.1. Plant Material Preparation and RNA-Seq
4.2. Differential Expression Analysis and Validation of P450s
4.3. Arabidopsis Transformation with P450 Genes in B. syzigachne
4.4. Resistance Identification of Transgenic Plants
4.5. Subcellular Localization of CYP99A44 and CYP704A177
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene ID | Function Annotation | RNA-Seq | Fold Change: qRT-PCR | |||
---|---|---|---|---|---|---|
Log2Fold Change | p Value | 12 h | 24 h | 48 h | ||
TRINITY_DN11725_c0_g1 | CYP71C4 | 9.32 | 7.83 × 10−22 | 1.02 | 0.1 | 71.01 * |
TRINITY_DN12889_c1_g2 | CYP99A44 | 7.98 | 2.49 × 10−14 | 1.45 | 1.53 | 764.04 * |
TRINITY_DN13855_c0_g2 | CYP72A15 | 7.53 | 3.05 × 10−18 | 1.11 | 1.64 | 15.3 * |
TRINITY_DN4168_c0_g1 | CYP71P11 | 6.52 | 1.95 × 10−24 | 4.08 * | 0.38 | 405.91 * |
TRINITY_DN12796_c0_g1 | CYP71D55 | 6.45 | 1.71 × 10−19 | 2.17 * | 0.2 | 72.38 * |
TRINITY_DN14232_c0_g3 | CYP71A1 | 6.19 | 1.59 × 10−30 | 1.34 | 2.61 * | 1.96 |
TRINITY_DN13855_c0_g1 | CYP72A14 | 5.85 | 1.37 × 10−22 | 0.18 | 0.44 | 42.49 * |
TRINITY_DN12677_c3_g5 | CYP71D55 | 5.76 | 1.80 × 10−12 | 0.78 | 1.21 | 2.33 * |
TRINITY_DN11507_c1_g9 | CYP93A1 | 5.63 | 1.49 × 10−07 | 2.04 * | 6.93 * | 376.11 * |
TRINITY_DN13492_c1_g1 | CYP71D7 | 5.51 | 4.32 × 10−11 | 8.38 * | 9.4 * | 596.34 * |
TRINITY_DN11999_c1_g1 | CYP71Z7 | 5.22 | 8.45 × 10−15 | 0.44 | 1.93 | 1.22 |
TRINITY_DN12352_c4_g3 | CYP89E28 | 5.1 | 4.31 × 10−07 | 2.11 * | 0.87 | 86.37* |
TRINITY_DN13661_c0_g3 | CYP72A14 | 5.05 | 3.19 × 10−33 | 1.44 | 0.77 | 1.34 |
TRINITY_DN11183_c0_g1 | CYP96B84 | 4.79 | 3.08 × 10−24 | 9.63 * | 66.26 * | 4837.35 * |
TRINITY_DN13666_c3_g5 | CYP94A1 | 4.51 | 2.53 × 10−09 | 0.99 | 1.21 | 1.36 |
TRINITY_DN11248_c6_g1 | CYP74A4 | 4.39 | 7.35 × 10−19 | 1.21 | 1.38 | 2.21 * |
TRINITY_DN13901_c6_g2 | CYP93A1 | 4.22 | 7.27 × 10−34 | 1.55 | 1.68 | 2.01 * |
TRINITY_DN13830_c0_g10 | CYP72A219 | 3.97 | 3.11 × 10−63 | 1.65 | 4.62 * | 13.43 * |
TRINITY_DN12172_c1_g2 | CYP734A6 | 3.78 | 1.64 × 10−15 | 2.22 * | 1.25 | 1.64 |
TRINITY_DN12282_c2_g1 | CYP72A603 | 3.09 | 3.29 × 10−13 | 1.09 | 1.76 | 26.13 * |
TRINITY_DN8749_c0_g3 | CYP71A9 | 3.09 | 9.20 × 10−06 | 1.14 | 0.26 | 20.61 * |
TRINITY_DN13317_c1_g3 | CYP71A1 | 3.07 | 1.07 × 10−11 | 0.57 | 0.17 | 4.74 * |
TRINITY_DN12014_c1_g2 | CYP71Z7 | 2.97 | 1.78 × 10−38 | 1.43 | 2.24 * | 1.79 |
TRINITY_DN9718_c0_g3 | CYP76B6 | 2.86 | 2.18 × 10-4 | 1.21 | 1.65 | 1.77 |
TRINITY_DN11725_c0_g2 | CYP71C4 | 2.74 | 4.73 × 10−07 | 0.66 | 1.56 | 1.92 |
TRINITY_DN13955_c0_g1 | CYP99A2 | 2.61 | 1.56 × 10-2 | 0.36 | 0.79 | 1.65 |
TRINITY_DN8888_c0_g1 | CYP716B2 | 2.61 | 4.15 × 10-3 | 1.8 | 0.23 | 77.84 * |
TRINITY_DN12495_c1_g4 | CYP704A177 | 2.57 | 4.65 × 10−29 | 5.29 * | 4.45 * | 7.99 * |
TRINITY_DN12889_c0_g6 | CYP71D55 | 2.54 | 3.09 × 10−05 | 0.55 | 0.68 | 1.23 |
Arabidopsis | Mesosulfuron-Methyl | ||
---|---|---|---|
GR50 ± SE (g a.i./ha) 1 | GR90 ± SE (g a.i./ha) 2 | Resistance Index 3 | |
WT | 1.28 ± 0.15 | 8.99 ± 4.98 | 1.00 |
CYP99A44 | 2.88 ± 0.34 | 13.17 ± 3.46 | 2.25 |
CYP704A177 | 1.83 ± 0.76 | 5.85 ± 1.54 | 1.43 |
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Bai, S.; Yin, M.; Lyu, Q.; Jiang, B.; Li, L. Cytochrome P450 BsCYP99A44 and BsCYP704A177 Confer Metabolic Resistance to ALS Herbicides in Beckmannia syzigachne. Int. J. Mol. Sci. 2022, 23, 12175. https://doi.org/10.3390/ijms232012175
Bai S, Yin M, Lyu Q, Jiang B, Li L. Cytochrome P450 BsCYP99A44 and BsCYP704A177 Confer Metabolic Resistance to ALS Herbicides in Beckmannia syzigachne. International Journal of Molecular Sciences. 2022; 23(20):12175. https://doi.org/10.3390/ijms232012175
Chicago/Turabian StyleBai, Shuang, Mengjie Yin, Qinghao Lyu, Bo Jiang, and Lingxu Li. 2022. "Cytochrome P450 BsCYP99A44 and BsCYP704A177 Confer Metabolic Resistance to ALS Herbicides in Beckmannia syzigachne" International Journal of Molecular Sciences 23, no. 20: 12175. https://doi.org/10.3390/ijms232012175