Hypoxia Inhibits Subretinal Inflammation Resolution Thrombospondin-1 Dependently
Abstract
:1. Introduction
2. Material and Methods
2.1. Laser-Injury Model, Intravitreal Injections, and Hypoxia
2.2. Monocyte RPE Co-Cultures
2.3. Gene Expression Analysis
2.4. Immunohistochemistry, CNV and MPs Quantification
2.5. Statistical Analyses
3. Results
3.1. Hypoxia Increases the Infiltration of Subretinal MPs after Laser-Injury
3.2. Hypoxia Decreases Thbs1-Expression in Mos
3.3. TSP-1 Is Necessary for Hypoxia-Induced Inhibition of Inflammation Resolution after Laser-Injury
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Acknowledgments
Conflicts of Interest
References
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Touhami, S.; Béguier, F.; Yang, T.; Augustin, S.; Roubeix, C.; Blond, F.; Conart, J.B.; Sahel, J.A.; Bodaghi, B.; Delarasse, C.; et al. Hypoxia Inhibits Subretinal Inflammation Resolution Thrombospondin-1 Dependently. Int. J. Mol. Sci. 2022, 23, 681. https://doi.org/10.3390/ijms23020681
Touhami S, Béguier F, Yang T, Augustin S, Roubeix C, Blond F, Conart JB, Sahel JA, Bodaghi B, Delarasse C, et al. Hypoxia Inhibits Subretinal Inflammation Resolution Thrombospondin-1 Dependently. International Journal of Molecular Sciences. 2022; 23(2):681. https://doi.org/10.3390/ijms23020681
Chicago/Turabian StyleTouhami, Sara, Fanny Béguier, Tianxiang Yang, Sébastien Augustin, Christophe Roubeix, Frederic Blond, Jean Baptiste Conart, José Alain Sahel, Bahram Bodaghi, Cécile Delarasse, and et al. 2022. "Hypoxia Inhibits Subretinal Inflammation Resolution Thrombospondin-1 Dependently" International Journal of Molecular Sciences 23, no. 2: 681. https://doi.org/10.3390/ijms23020681