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Article

Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac Progenitor Specification during Mouse Gastrulation

1
Novo Nordisk Foundation Center for Stem Cell Biology (DanStem), University of Copenhagen, 2200 Copenhagen, Denmark
2
Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense, Denmark
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: J. Carlos Villaescusa
Int. J. Mol. Sci. 2022, 23(18), 10307; https://doi.org/10.3390/ijms231810307
Received: 8 August 2022 / Revised: 30 August 2022 / Accepted: 31 August 2022 / Published: 7 September 2022
Progesterone treatment is commonly employed to promote and support pregnancy. While maternal tissues are the main progesterone targets in humans and mice, its receptor (PGR) is expressed in the murine embryo, questioning its function during embryonic development. Progesterone has been previously associated with murine blastocyst development. Whether it contributes to lineage specification is largely unknown. Gastrulation initiates lineage specification and generation of the progenitors contributing to all organs. Cells passing through the primitive streak (PS) will give rise to the mesoderm and endoderm. Cells emerging posteriorly will form the extraembryonic mesodermal tissues supporting embryonic growth. Cells arising anteriorly will contribute to the embryonic heart in two sets of distinct progenitors, first (FHF) and second heart field (SHF). We found that PGR is expressed in a posterior–anterior gradient in the PS of gastrulating embryos. We established in vitro differentiation systems inducing posterior (extraembryonic) and anterior (cardiac) mesoderm to unravel PGR function. We discovered that PGR specifically modulates extraembryonic and cardiac mesoderm. Overexpression experiments revealed that PGR safeguards cardiac differentiation, blocking premature SHF progenitor specification and sustaining the FHF progenitor pool. This role of PGR in heart development indicates that progesterone administration should be closely monitored in potential early-pregnancy patients undergoing infertility treatment. View Full-Text
Keywords: progesterone receptor; cardiac differentiation; mesoderm induction; mouse gastrulation; epiblast stem cells; extraembryonic mesoderm; cell adhesion; ECM progesterone receptor; cardiac differentiation; mesoderm induction; mouse gastrulation; epiblast stem cells; extraembryonic mesoderm; cell adhesion; ECM
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MDPI and ACS Style

Drozd, A.M.; Mariani, L.; Guo, X.; Goitea, V.; Menezes, N.A.; Ferretti, E. Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac Progenitor Specification during Mouse Gastrulation. Int. J. Mol. Sci. 2022, 23, 10307. https://doi.org/10.3390/ijms231810307

AMA Style

Drozd AM, Mariani L, Guo X, Goitea V, Menezes NA, Ferretti E. Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac Progenitor Specification during Mouse Gastrulation. International Journal of Molecular Sciences. 2022; 23(18):10307. https://doi.org/10.3390/ijms231810307

Chicago/Turabian Style

Drozd, Anna Maria, Luca Mariani, Xiaogang Guo, Victor Goitea, Niels Alvaro Menezes, and Elisabetta Ferretti. 2022. "Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac Progenitor Specification during Mouse Gastrulation" International Journal of Molecular Sciences 23, no. 18: 10307. https://doi.org/10.3390/ijms231810307

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