Mechanical Stretch-Induced NLRP3 Inflammasome Expression on Human Annulus Fibrosus Cells Modulated by Endoplasmic Reticulum Stress
Abstract
:1. Introduction
2. Results
2.1. The 15% HCS Induced NLRP3 Expression in Human AF Cells
2.2. The 15% HCS Induced the Expression of IL-1β in Human AF Cells
2.3. Effects of 15% HCS on NOX2 Expression and ROS Production in Human AF Cells
2.4. ER Stress Regulates NOX2 Expression in Human AF Cells under 15% HCS
2.5. TXNIP and NLRP3 Expression Is Regulated by NOX2 and ER Stress in Human AF Cells under 15% HCS
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Culture of AF Cell Lines
4.3. Cyclic Stretching
4.4. Real-Time Quantitative PCR
4.5. IL-1β Enzyme-Linked Immunosorbent Assay (ELISA)
4.6. Western Blot Analysis
4.7. siRNA Transfection
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chang, H.-I.; Chen, C.-N.; Huang, K.-Y. Mechanical Stretch-Induced NLRP3 Inflammasome Expression on Human Annulus Fibrosus Cells Modulated by Endoplasmic Reticulum Stress. Int. J. Mol. Sci. 2022, 23, 7951. https://doi.org/10.3390/ijms23147951
Chang H-I, Chen C-N, Huang K-Y. Mechanical Stretch-Induced NLRP3 Inflammasome Expression on Human Annulus Fibrosus Cells Modulated by Endoplasmic Reticulum Stress. International Journal of Molecular Sciences. 2022; 23(14):7951. https://doi.org/10.3390/ijms23147951
Chicago/Turabian StyleChang, Hsin-I, Cheng-Nan Chen, and Kuo-Yuan Huang. 2022. "Mechanical Stretch-Induced NLRP3 Inflammasome Expression on Human Annulus Fibrosus Cells Modulated by Endoplasmic Reticulum Stress" International Journal of Molecular Sciences 23, no. 14: 7951. https://doi.org/10.3390/ijms23147951