Enhancing Animal Disease Resistance, Production Efficiency, and Welfare through Precise Genome Editing
Abstract
:1. Introduction
2. CRISPR/Cas System Mediated Genetic Manipulation
3. Disease Resistance Breeding
4. Improving Production Performance
5. Improving Animal Welfare
6. Regulations on GEAs
7. Prospects and Challenges
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Species | Gene | Modification * | Method * | Applications | References |
---|---|---|---|---|---|
Pig | RELA | KO | ZFN | Disease resistance | [79] |
CD169 | KO | HR | Disease resistance | [27] | |
CD163 | KO | CRISPR/Cas9 | Disease resistance | [29,30,31] | |
CD163 (SRCR 5 domain) | hCD163L1 SRCR domain 8 replacement | CRISPR/Cas9 | Disease resistance | [32,33] | |
CD163 (SRCR 5 domain) | Domain deletion | CRISPR/Cas9 | Disease resistance | [34,35,36] | |
HDAC6 | TG | SCNT | Disease resistance | [47] | |
CD163 | Partial domain deletion | CRISPR/Cas9 | Disease resistance | [37] | |
pAPN | KO | CRISPR/Cas9 | Disease resistance | [40] | |
CD163, pAPN | Double KO | CRISPR/Cas9 | Disease resistance | [42] | |
shRNA | TG | Injection | Disease resistance | [22] | |
CMAH | KO | CRISPR/Cas9 | Disease resistance | [43] | |
IGF2 | Regulatory element mutation | CRISPR/Cas9 | Meat production | [69,70] | |
IRX3 | KO | CRISPR/Cas9 | Fat content | [80] | |
FBXO40 | KO | CRISPR/Cas9 | Meat production | [81] | |
PBD-2 | KI | CRISPR/Cas9 | Disease resistance | [82] | |
MSTN | Point mutation | CRISPR/Cas9 | Meat production | [65] | |
MSTN | Partial deletion | CRISPR/Cpf1 | Meat production | [66] | |
FST | KI | CRISPR/Cas9 | Meat production | [67] | |
MSTN | KO | CRISPR/Cas9 | Meat production | [62] | |
RSAD2 | KI | CRISPR/Cas9 | Disease resistance | [83] | |
fat-1 | KI | CRISPR/Cas9 | Meat quality | [73] | |
fat-1, IGF1 | Double KI | CRISPR/Cas9 | Meat quality and meat production | [75] | |
UCP1 | KI | CRISPR/Cas9 | Fat content and animal welfare | [76] | |
KISSR | KO | CRISPR/Cas9 | Animal welfare | [78] | |
Cattle | SP110 | KI | TALENs | Disease resistance | [45] |
NRAMP1/SLC11A1 | KI | CRISPR/Cas9 | Disease resistance | [46] | |
CD18 | Point mutation | ZFN | Disease resistance | [24] | |
IARS | KI | CRISPR/Cas9 | Animal welfare | [84] | |
POLLED allele | KI | TALENs | Animal welfare | [77] | |
Sheep | BMPR1B | Point mutation | CRISPR/Cas9 | Reproductive traits | [85] |
MSTN | KO | CRISPR/Cas9 | Meat production | [60] | |
SOCS2 | Point mutation | Base Editor | Growth rate | [72] | |
Goat | GDF9 | KI | CRISPR/Cas9 | Reproductive traits | [86] |
fat-1, MSTN | KI and KO | CRISPR/Cas9 | Meat quality and meat production | [74] | |
MSTN | KO | CRISPR/Cas9 | Meat production | [61] |
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Liu, Z.; Wu, T.; Xiang, G.; Wang, H.; Wang, B.; Feng, Z.; Mu, Y.; Li, K. Enhancing Animal Disease Resistance, Production Efficiency, and Welfare through Precise Genome Editing. Int. J. Mol. Sci. 2022, 23, 7331. https://doi.org/10.3390/ijms23137331
Liu Z, Wu T, Xiang G, Wang H, Wang B, Feng Z, Mu Y, Li K. Enhancing Animal Disease Resistance, Production Efficiency, and Welfare through Precise Genome Editing. International Journal of Molecular Sciences. 2022; 23(13):7331. https://doi.org/10.3390/ijms23137331
Chicago/Turabian StyleLiu, Zhiguo, Tianwen Wu, Guangming Xiang, Hui Wang, Bingyuan Wang, Zheng Feng, Yulian Mu, and Kui Li. 2022. "Enhancing Animal Disease Resistance, Production Efficiency, and Welfare through Precise Genome Editing" International Journal of Molecular Sciences 23, no. 13: 7331. https://doi.org/10.3390/ijms23137331