Dynamics of Protein Phosphorylation during Arabidopsis Seed Germination
Abstract
:1. Introduction
2. Results
2.1. A Large Repertoire of Phosphoproteins Identified in Dry and Imbibed Arabidopsis Seeds
2.2. Modification of the Composition of Seed Phosphoproteome in Dark-Imbibed and Light-Imbibed Seeds
2.3. Modifications of the Abundance of Constitutive Seed Phosphoproteins in Dark-Imbibed and Light-Imbibed Seeds
3. Discussion
4. Materials and Methods
4.1. Seed Material
4.2. Germination Assays
4.3. Protein Extraction and In-Solution Digestion
4.4. Stable Isotope Dimethyl Labeling for Phosphoproteomic Approach
4.5. Peptide Fractionation Using Strong Cation Exchange Chromatography (SCX)
4.6. Enrichment of Phosphopeptides Using Immobilized Metal Ion Affinity Chromatography (IMAC)
4.7. nanoLC-MS/MS Analysis
4.8. Identification of Proteins and Phosphopeptides
4.9. Quantification of Peptides and Phosphopeptides
4.10. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Baudouin, E.; Puyaubert, J.; Meimoun, P.; Blein-Nicolas, M.; Davanture, M.; Zivy, M.; Bailly, C. Dynamics of Protein Phosphorylation during Arabidopsis Seed Germination. Int. J. Mol. Sci. 2022, 23, 7059. https://doi.org/10.3390/ijms23137059
Baudouin E, Puyaubert J, Meimoun P, Blein-Nicolas M, Davanture M, Zivy M, Bailly C. Dynamics of Protein Phosphorylation during Arabidopsis Seed Germination. International Journal of Molecular Sciences. 2022; 23(13):7059. https://doi.org/10.3390/ijms23137059
Chicago/Turabian StyleBaudouin, Emmanuel, Juliette Puyaubert, Patrice Meimoun, Mélisande Blein-Nicolas, Marlène Davanture, Michel Zivy, and Christophe Bailly. 2022. "Dynamics of Protein Phosphorylation during Arabidopsis Seed Germination" International Journal of Molecular Sciences 23, no. 13: 7059. https://doi.org/10.3390/ijms23137059