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Article

Neuroprotective Effect of Macrophage Migration Inhibitory Factor (MIF) in a Mouse Model of Ischemic Stroke

1
Asan Medical Center, Department of Rehabilitation Medicine, University of Ulsan College of Medicine, Seoul 05505, Korea
2
Asan Medical Center, Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul 05505, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Fabio Cavaliere
Int. J. Mol. Sci. 2022, 23(13), 6975; https://doi.org/10.3390/ijms23136975
Received: 31 May 2022 / Revised: 17 June 2022 / Accepted: 20 June 2022 / Published: 23 June 2022
(This article belongs to the Section Molecular Neurobiology)
The mechanism of the neuroprotective effect of the macrophage migration inhibitory factor (MIF) in vivo is unclear. We investigated whether the MIF promotes neurological recovery in an in vivo mouse model of ischemic stroke. Transient middle cerebral artery occlusion (MCAO) surgery was performed to make ischemic stroke mouse model. Male mice were allocated to a sham vehicle, a sham MIF, a middle cerebral artery occlusion (MCAO) vehicle, and MCAO+MIF groups. Transient MCAO (tMCAO) was performed in the MCAO groups, and the vehicle and the MIF were administered via the intracerebroventricular route. We evaluated the neurological functional scale, the rotarod test, and T2-weighted magnetic resonance imaging. The expression level of the microtubule-associated protein 2 (MAP2), Bcl2, and the brain-derived neurotrophic factor (BDNF) were further measured by Western blot assay. The Garcia test was significantly higher in the MCAO+MIF group than in the MCAO+vehicle group. The MCAO+MIF group exhibited significantly better performance on the rotarod test than the MCAO+vehicle group, which further had a significantly reduced total infarct volume on T2-weighted MRI imaging than the MCAO vehicle group. Expression levels of BDNF, and MAP2 tended to be higher in the MCAO+MIF group than in the MCAO+vehicle group. The MIF exerts a neuroprotective effect in an in vivo ischemic stroke model. The MIF facilitates neurological recovery and protects brain tissue from ischemic injury, indicating a possibility of future novel therapeutic agents for stroke patients. View Full-Text
Keywords: macrophage migration inhibitory factor; stroke; in vivo; neurogenesis macrophage migration inhibitory factor; stroke; in vivo; neurogenesis
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MDPI and ACS Style

Kim, J.A.; Kim, Y.Y.; Lee, S.H.; Jung, C.; Kim, M.H.; Kim, D.Y. Neuroprotective Effect of Macrophage Migration Inhibitory Factor (MIF) in a Mouse Model of Ischemic Stroke. Int. J. Mol. Sci. 2022, 23, 6975. https://doi.org/10.3390/ijms23136975

AMA Style

Kim JA, Kim YY, Lee SH, Jung C, Kim MH, Kim DY. Neuroprotective Effect of Macrophage Migration Inhibitory Factor (MIF) in a Mouse Model of Ischemic Stroke. International Journal of Molecular Sciences. 2022; 23(13):6975. https://doi.org/10.3390/ijms23136975

Chicago/Turabian Style

Kim, Ji Ae, Ye Young Kim, Seung Hak Lee, Chul Jung, Mi Hee Kim, and Dae Yul Kim. 2022. "Neuroprotective Effect of Macrophage Migration Inhibitory Factor (MIF) in a Mouse Model of Ischemic Stroke" International Journal of Molecular Sciences 23, no. 13: 6975. https://doi.org/10.3390/ijms23136975

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