Genome-Wide Analysis of the Peptidase M24 Superfamily in Triticum aestivum Demonstrates That TaM24-9 Is Involved in Abiotic Stress Response
Abstract
:1. Introduction
2. Results
2.1. Identification and Characterization of TaM24 Genes
2.2. Phylogenetic Analysis and Classification of TaM24 Proteins
2.3. Chromosome Mapping and Homologous Gene Identification of TaM24 Genes
2.4. Analyzing Gene Duplication Events and Natural Selection
2.5. Motif Analysis and Gene Structure Analysis
2.6. Gene Ontology (GO) Analysis and Protein Interaction Network (PPI) Analysis
2.7. Cis-Acting Element Analysis of Promoter Region
2.8. Analysis of Expression Patterns of TaM24 Genes
2.9. Overexpression of TaM24-9 Affects Seed Germination under Drought or NaCl Stress in Arabidopsis
2.10. Overexpression of TaM24-9 Affects Growth State of Arabidopsis at the Seedling Stage under Drought and NaCl Stress
3. Discussion
4. Materials and Methods
4.1. Identification of Peptidase M24 Gene Family Members in Wheat
4.2. Phylogenetic Analysis and Classification of TaM24 Proteins
4.3. Chromosomal Location and Identification of Homoeologs
4.4. Gene Duplication and Evolutionary Selection Analysis
4.5. Gene Structure and Conserved Motif Analysis
4.6. Cis-Acting Element Analysis
4.7. Gene Ontology (GO) Analysis and Protein Interaction Network (PPI) Analysis
4.8. Expression Analysis of TaM24 Genes
4.9. RNA Extraction and Quantitative Real-Time PCR
4.10. Plant Materials, Growth Conditions, and Stress Treatments
4.11. Measurement of Malondialdehyde and Proline Contents
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Homoeologous Group (A:B:D) | All Wheat Genes | All TaM24 Genes | ||
---|---|---|---|---|
Number of Groups | Number of Genes | % of Genes | ||
1:1:1 | 35.80% | 12 | 36 | 68% |
1:1:n/1:n:1/n:1:1, n > 1 | 5.70% | 3 | 14 | 26% |
1:1:0/1:0:1/0:1:1 | 13.20% | 0 | 0 | 0% |
Orphans/singletons | 37.10% | - | 3 | 6% |
Other rations | 8.00% | 0 | 0 | 0% |
Total | 99.80% | - | 53 | 100% |
MOTIF | Sequences | WIDTH |
---|---|---|
1 | TDEIDRAVHQMIIDAGAYPSPLGYGGFPKSVCTSVNECICH | 41 |
2 | GIPDDRTLQDGDIINIDVGVYYNGYHSDTSRTY | 33 |
3 | FNWEKIEMYKSFGGVRIESNLYVTAQGCKNLTNCPRETWEIEAVMAGAPW | 50 |
4 | ISHMQPGVKWIDMHKLAEQRILESLKKENIIHGDIGDMMNRRLGAVFMPH | 50 |
5 | WTAVTADGSLSAQFEHTVLVTETGAEVLT | 29 |
6 | SYFAYLFGVREPGFYGAVDIASGQSILFAP | 30 |
7 | LKEGMVFTVEPGLYF | 15 |
8 | KVTKEAMELAISACKPGVSFKAIGEVISK | 29 |
9 | PLLFLLYGKNTDSGNYSKPASFEGIEKFDTDLSTLHPILTEC | 42 |
10 | PVPEHIPRPPYVGSBKLPEVNPDRQMHDREGIVHMRAACELAARVLQFAG | 59 |
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Yan, L.-Y.; Guo, J.-G.; Zhang, X.; Liu, Y.; Xiong, X.-X.; Han, Y.-X.; Zhang, L.-L.; Zhang, X.-H.; Min, D.-H. Genome-Wide Analysis of the Peptidase M24 Superfamily in Triticum aestivum Demonstrates That TaM24-9 Is Involved in Abiotic Stress Response. Int. J. Mol. Sci. 2022, 23, 6904. https://doi.org/10.3390/ijms23136904
Yan L-Y, Guo J-G, Zhang X, Liu Y, Xiong X-X, Han Y-X, Zhang L-L, Zhang X-H, Min D-H. Genome-Wide Analysis of the Peptidase M24 Superfamily in Triticum aestivum Demonstrates That TaM24-9 Is Involved in Abiotic Stress Response. International Journal of Molecular Sciences. 2022; 23(13):6904. https://doi.org/10.3390/ijms23136904
Chicago/Turabian StyleYan, Lu-Yu, Jia-Gui Guo, Xin Zhang, Yang Liu, Xin-Xin Xiong, Yu-Xuan Han, Li-Li Zhang, Xiao-Hong Zhang, and Dong-Hong Min. 2022. "Genome-Wide Analysis of the Peptidase M24 Superfamily in Triticum aestivum Demonstrates That TaM24-9 Is Involved in Abiotic Stress Response" International Journal of Molecular Sciences 23, no. 13: 6904. https://doi.org/10.3390/ijms23136904