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Article

Long Non-Coding RNA MIR31HG Promotes the Transforming Growth Factor β-Induced Epithelial-Mesenchymal Transition in Pancreatic Ductal Adenocarcinoma Cells

by
Ching-Chung Ko
1,2,3,
Yao-Yu Hsieh
4,5 and
Pei-Ming Yang
6,7,8,9,10,*
1
Department of Medical Imaging, Chi Mei Medical Center, Tainan 71004, Taiwan
2
Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan
3
Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan
4
Division of Hematology and Oncology, Taipei Medical University Shuang Ho Hospital, New Taipei City 23561, Taiwan
5
Division of Hematology and Oncology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
6
Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan
7
PhD Program for Cancer Molecular Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei 11031, Taiwan
8
TMU Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 11031, Taiwan
9
Cancer Center, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan
10
TMU and Affiliated Hospitals Pancreatic Cancer Groups, Taipei Medical University, Taipei 11031, Taiwan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(12), 6559; https://doi.org/10.3390/ijms23126559
Submission received: 5 May 2022 / Revised: 10 June 2022 / Accepted: 10 June 2022 / Published: 12 June 2022
(This article belongs to the Special Issue Non-coding RNA (ncRNA) in Cancer)
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Abstract

:
The epithelial-to-mesenchymal transition (EMT) describes a biological process in which polarized epithelial cells are converted into highly motile mesenchymal cells. It promotes cancer cell dissemination, allowing them to form distal metastases, and also involves drug resistance in metastatic cancers. Transforming growth factor β (TGFβ) is a multifunctional cytokine that plays essential roles in development and carcinogenesis. It is a major inducer of the EMT. The MIR31 host gene (MIR31HG) is a newly identified long non-coding (lnc)RNA that exhibits ambiguous roles in cancer. In this study, a cancer genomics analysis predicted that MIR31HG overexpression was positively correlated with poorer disease-free survival of pancreatic ductal adenocarcinoma (PDAC) patients, which was associated with upregulation of genes related to TGFβ signaling and the EMT. In vitro evidence demonstrated that TGFβ induced MIR31HG expression in PDAC cells, and knockdown of MIR31HG expression reversed TGFβ-induced EMT phenotypes and cancer cell migration. Therefore, MIR31HG has an oncogenic role in PDAC by promoting the EMT.

1. Introduction

In 2020, pancreatic cancer became the seventh leading cause of cancer mortality worldwide, and there are nearly as many pancreatic cancer patient deaths (466,000) as there are new cases (496,000) due to its poor prognosis [1]. Pancreatic ductal adenocarcinoma (PDAC) accounts for more than 85% of patients, and it is the most prevalent and aggressive pancreatic cancer type [2]. PDAC has a very low 5-year survival rate (of <8%) because of late diagnoses, high rates of metastasis, and drug resistance [3,4]. The recurrence rate remains significant even in patients with early-stage disease [5]. The majority of PDAC patients (80%~85%) are not suitable for surgical resection, which is the only curative therapeutic option. Furthermore, approximately half of PDAC patients have metastases when first diagnosed, also negating curative resection [4]. Gemcitabine-based chemotherapy is indispensable for treating unresectable pancreatic cancer [5]. However, current regimens are not satisfactory because PDAC is one of the most chemoresistant cancer types [6]. Therefore, obtaining a better understanding of pancreatic cancer biology will help in developing novel therapeutic strategies.
Dysregulation of long non-coding (lnc)RNAs is frequently found in cancers, which reveals new targets for interventions [7,8]. The MIR31 host gene (MIR31HG) is an lncRNA located on human chromosome 9 (9p21.3). Although MIR31HG is the host gene of microRNA (miR)-31 and their expressions are positively correlated in some cancers, knockdown of MIR31HG does not change the level of miR-31 [9,10], suggesting that MIR31HG may function in cancers independent of miR-31. MIR31HG is frequently upregulated in various cancer types, including PDAC, and serves as an oncogene and a poor prognostic factor [9,10,11,12,13,14,15,16,17,18,19,20]. Previously identified MIR31HG targets include hypoxia-inducible factor (HIF)-1α, p21, miR-193b, miR-214, miR-361-3p, and miR-761, and these are associated with tumor growth, metastasis, and chemoresistance [10,11,12,14,17,20,21]. As a co-activator of HIF-1α, MIR31HG is also named long non-coding HIF-1α co-activating RNA (lncHIFCAR) [14]. Controversially, MIR31HG is downregulated in bladder cancer, esophageal squamous cell carcinoma, and hepatocellular carcinoma [22,23,24]. Therefore, the role of MIR31HG in cancers might be cancer type-specific.
The epithelial-to-mesenchymal transition (EMT) describes a biological process in which polarized epithelial cells are converted into highly motile mesenchymal cells. Loss of cell–cell adhesion and related markers such as E-cadherin (CDH1), upregulation of mesenchymal markers such as vimentin (VIM) and N-cadherin (CDH2), and acquisition of a motile capacity and a fibroblast-like phenotype, are major hallmarks of the EMT [25,26]. The EMT plays an important role during embryogenesis, such as facilitating the generation of new tissues and organs. It also contributes to the pathogenesis of tissue fibrosis, tumor progression, and metastasis [27,28,29]. One of the most distinguishing and significant characteristics of pancreatic cancer is the EMT. It occurs even in pancreatic intraepithelial neoplasia (PanIN), the histological precursor to invasive PDAC, and leads to early dissemination, drug resistance, and a poor prognosis [30]. Although the precise role of the EMT in the pancreatic cancer cells’ biological behaviors and its implications for clinical therapy remain controversial, a therapeutic strategy of combining EMT inhibition with chemotherapy is worth considering [31].
In this study, cancer genomics data mining revealed that MIR31HG overexpression was positively associated with poorer disease-free survival and a transforming growth factor β (TGFβ)-induced EMT gene signature in PDAC patients. In vitro experiments demonstrated that TGFβ induced MIR31HG expression and promoted the TGFβ-induced EMT in PDAC cells. Our results support the oncogenic role of MIR31HG in PDAC.

2. Results

2.1. Upregulation of MIR31HG Is Associated with Disease-Free Survival in PDAC Patients

Previously, only one study investigated the role of MIR31HG in pancreatic cancer [10], which found that MIR31HG is overexpressed in PDAC tissues and cell lines. Downregulation of MIR31HG inhibits in vitro and in vivo PDAC cell growth by repressing cell cycle progression and inducing apoptosis. In addition, they identified that a tumor-suppressive miR-193b directly targets MIR31HG, and MIR31HG also competes for miR-193b binding to its messenger (m)RNA targets [10]. To obtain a greater understanding of the role of MIR31HG in PDAC, MIR31HG expressions in normal and PDAC tissues were obtained from the Gene Expression Profiling Interactive Analysis (GEPIA) database [32]. As shown in Figure 1A, MIR31HG was overexpressed in PDAC tumor tissues according to The Cancer Genome Atlas (TCGA) PanCancer Atlas dataset [33]. Because only four normal pancreatic tissues existed in this dataset and an additional 167 normal pancreatic tissues were from Genotype-Tissue Expression (GTEx) data [34], two other pancreatic cancer cohorts (GSE16515 [35] and GSE28735 [36]) were obtained from the Gene Expression Omnibus (GEO) database [37]. Consistently, MIR31HG was found to be overexpressed in tumor tissues in these two cohorts (Figure 1B). To investigate the prognostic role of MIR31HG in PDAC patients, Kaplan-Meier overall and disease-free survival plots correlated with MIR31HG expression were obtained from the GEPIA and PROGgeneV2 databases [38]. As shown in Figure 1C and 1D, MIR31HG overexpression was not correlated with overall survival in either TCGA or GSE28735 datasets. However, MIR31HG expression was correlated with disease-free survival in the TCGA dataset, suggesting that MIR31HG overexpression may be correlated with recurrence and metastasis in PDAC patients. Because the disease-free survival status in the GSE28735 cohort was not available, whether MIR31HG overexpression was also correlated with patient’s disease-free survival was unknown.

2.2. Upregulation of MIR31HG Is Associated with the EMT Gene Signature in PDAC Patients

To investigate molecular alterations correlated with MIR31HG overexpression in PDAC, microarray gene expression profiles in GSE16515 and GSE28735 were analyzed using Gene Set Enrichment Analysis (GSEA) software [39,40] for enrichment of cancer hallmarks [41]. We found that seven cancer hallmarks, including TGFβ signaling, were commonly enriched in the two PDAC cohorts (Figure 2A). TGFβ signaling has both protumorigenic and tumor-suppressive roles in PDAC, which depends on the tumor stage [42,43]. TGFβ suppresses tumors in the early stages of carcinogenesis, because it serves as an antimitogen that stops cell-cycle progression during the G1 phase [44]. During pancreatic carcinogenesis, changes in TGFβ signaling components are prevalent. For example, mutations in the SMAD4 and TGFβ type II receptor (TGFBRII) genes may make cancer cells resistant to TGFβ’s antimitogenic activity [42,43]. Thus, TGFβ promotes cancer invasion, angiogenesis, and metastasis by inducing the EMT in late-stage carcinogenesis [42,43]. Although the EMT cancer hallmark was not commonly enriched in these two pancreatic cancer cohorts, we found that the EMT tended to be enriched in MIR31HG-high expressing pancreatic cancer patients (Figure 2B). Therefore, MIR31HG overexpression was correlated with the EMT gene signature in PDAC. Consistent with our study, colorectal cancer patients with higher MIR31HG expression were characterized by elevated TGFβ and EMT gene expressions [15].

2.3. Upregulation of MIR31HG Is Associated with the TGFβ-Induced EMT in PANC-1 Cells

lncRNAs, such as MALAT1, HOTAIR, H19, LncRNA-ATB, and LincRNA-ROR, are involved in the EMT through cross-talk with their master regulators [45,46]. However, the role of MIR31HG in the EMT is still unclear. To investigate the role of MIR31HG in the TGFβ-induced EMT, a microarray dataset (GSE23952 [47]) from TGFβ-treated PANC-1 cells was obtained from the GEO database. Relative mRNA expression levels of MIR31HG and EMT markers were visualized as a heat map (Figure 3A). We found that MIR31HG expression was higher during the TGFβ-induced EMT in PANC-1 cells, along with induction of mesenchymal markers (zinc finger E-box binding homeobox 1 (ZEB1), ZEB2, Snail (SNAI1), Slug (SNAI2), twist family bHLH transcription factor 1 (TWIST1), VIM, CDH2, fibronectin 1 (FN1), and collagen type I alpha 1 chain (COL1A1)) and reduction of epithelial markers (CDH1, P-cadherin (CDH3), plakoglobin (JUP), desmoplakin (DSP), plakophilin 2 (PKP2), claudin 3 (CLDN3), and CLDN4) (Figure 3A). To confirm this observation, PANC-1 cells were challenged with TGFβ, and selected EMT markers were detected by real-time quantitative polymerase chain reaction (qPCR) and Western blot analyses. As shown in Figure 3B, TGFβ indeed induced MIR31HG in PANC-1 cells. Induction of the EMT was ascertained by the upregulation of VIM, CDH2, COL1A1, SNAI1, and SNAI2, and the downregulation of CDH1, CLDN4, and JUP at the mRNA (Figure 3B) and/or protein levels (Figure 3C). Cell morphological changes further confirmed this phenomenon. Untreated cells showed a pebble-like shape and cell–cell adhesion. After treatment with TGFβ for 72 h, cells had converted to a spindle-shaped, fibroblast-like morphology (Figure 3D). Therefore, MIR31HG was induced during the TGFβ-induced EMT.

2.4. MIR31HG Enhances the TGFβ-Induced EMT in PANC-1 Cells

To investigate whether MIR31HG participates in the TGFβ-induced EMT, PANC-1 cells were transfected with MIR31HG small interfering (si)RNA and then challenged with TGFβ. Cell morphological observations showed that cells had converted to a spindle-shaped, fibroblast-like morphology by 48 h TGFβ treatment, which was prevented by MIR31HG knockdown (Figure 4A). Real-time qPCR and Western blot analyses showed that MIR31HG knockdown suppressed TGFβ-induced downregulation of CDH1 and CLDN4 and upregulation of COL1A1 at both the mRNA and protein levels (Figure 4B,C). On the other hand, MIR31HG overexpression slightly enhanced 24 h TGFβ treatment-induced cell morphological changes and upregulation of COL1A1 and VIM at the mRNA or protein level (Figure 4D–F). However, MIR31HG knockdown or overexpression alone was insufficient to induce significant changes in cell morphology and EMT marker expressions (Figure 4), suggesting that MIR31HG participates in the EMT when TGFβ signaling is activated. Acquisition of a motile capacity is an important feature of the EMT [48]. Thus, the migrating activity of PANC-1 cells was measured by a wound-healing assay. As shown in Figure 5, TGFβ increased the cell-migrating activity, which was rescued by silencing the MIR31HG expression. Consistently, MIR31HG knockdown alone did not affect cell-migratory activity.

3. Discussion

Depending on the cancer type, MIR31HG can be either oncogenic or tumor-suppressive [9,10,11,12,13,14,15,16,17,18,19,20,22,23,24]. For example, MIR31HG inhibits cell proliferation and metastasis in hepatocellular carcinoma [24]. In contrast, MIR31HG acts as an HIF-1α co-activator and drives oral cancer progression [14]. The oncogenic property of MIR31HG was also identified in PDAC, in which MIR31HG promotes cancer cell proliferation and invasion [10]. Our results also support the oncogenic role of MIR31HG in PDAC by enhancing the TGFβ-induced EMT.
Cancer cells employ the EMT to gain invasive ability [48]. It was shown that MIR31HG knockdown inhibited the invasion of AsPC-1 and PANC-1 pancreatic cancer cells [10]. Whether MIR31HG knockdown also inhibits TGFβ-induced cell invasion warrants further investigation. The above study together with ours also implies that the effects of MIR31HG on cancer cell migration and invasion are uncoupled as reported earlier in other cancer cell types [49,50]. In addition, previous studies in non-small cell lung cancer and osteosarcoma cells also identified a promoting role of MIR31HG in the EMT [51,52]. However, they found that MIR31HG knockdown was sufficient to upregulate mesenchymal markers and downregulate epithelial markers [52], suggesting that a cancer type-specific role of MIR31HG may exist.
Our results indicated that MIR31HG alone did not seem to impact the EMT phenotype. However, loss-of-function and gain-of-function experiments indicated that COL1A1 gene expression was regulated by MIR31HG among the EMT markers tested in this study, suggesting that COL1A1 may be a potential target of MIR31HG. Interestingly, COL1A1 expression can be suppressed by miR-193 family members [53,54]. Because MIR31HG acts as an endogenous sponge of miR-193b in PDAC [10], we hypothesized that MIR31HG may also compete for miR-193 binding to COL1A1 mRNA.
In addition to its role in cancer, MIR31HG also plays a role in adipocyte differentiation (adipogenesis) [55]. Overexpression of MIR31HG reduces adipocyte differentiation in vitro and in vivo. In contrast, knockdown of MIR31HG inhibits the expression of an adipogenic-related gene, fatty acid binding protein 4 (FABP4), via histone modification of its gene promoter, leading to suppression of adipocyte differentiation [55]. Interestingly, it has been reported that EMT-derived breast cancer cells, but not epithelial cancer cells, can be trans-differentiated into post-mitotic and functional adipocytes, leading to the repression of primary tumor metastasis [56]. It would be interesting to explore whether such phenomena also exist in PDAC and the involving role of MIR31HG in cancer cell-adipocyte trans-differentiation in the future.
In conclusion, we found that MIR31HG overexpression in PDAC was positively associated with patients’ disease-free survival, but not overall survival. In addition, MIR31HG overexpression was correlated with upregulation of TGFβ signaling and the EMT. In vitro experiments confirmed the induction of MIR31HG by TGFβ treatment in PDAC cells. Knockdown of MIR31HG expression reversed the TGFβ-induced EMT. However, this study has several potential biases and limitations. First, the expression of MIR31HG and its clinical impact in PDAC patients were only analyzed using TCGA, GSE16515, and GSE28735 datasets. Further validation using clinical samples is still needed. Second, only one cell line was used in the in vitro experiments. Cell type variations may exist. In addition, animal experiments are needed to validate the in vitro observations. Third, the mechanisms of how TGFβ upregulates MIR31HG expression and how MIR31HG promotes the TGFβ-induced EMT have not yet been elucidated. Therefore, more investigations are required for a full understanding of the oncogenic role of MIR31HG in PDAC and a better validation of our conclusions.

4. Materials and Methods

4.1. Cell Culture and Treatment

A human pancreatic cancer cell line (PANC-1) from the Bioresource Collection and Research Center (BCRC; Hsinchu, Taiwan) was kindly provided by Prof. Hsin-Yi Chen (Taipei Medical University, Taipei, Taiwan). Cells were cultured in Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum (FBS), 2 mM L-glutamine, 1 mM sodium pyruvate, and 1% antibiotic-antimycotic, and were grown at 37 °C in a humidified CO2 (5%) incubator. For TGFβ treatment, cells were first serum-starved for 24 h and then treated with TGFβ in serum-free medium. The recombinant human TGFβ1 (#100-21) was purchased from PeproTech (Rocky Hill, NJ, USA). Cellular morphological changes were photographed at 20× magnification under an inverted microscope (IX71, Olympus, Tokyo, Japan).

4.2. Real-Time Quantitative Polymerase Chain Reaction (qPCR)

Total RNA was purified with a GENEzol TriRNA Pure Kit (#GZX100; Geneaid, New Taipei City, Taiwan). First-strand complementary (c)DNA was synthesized using an iScript cDNA Synthesis Kit (#1708891; Bio-Rad Laboratories, Hercules, CA, USA). PCR amplification with gene-specific primers (Table 1) was performed using the IQ2 SYBR Green Fast qPCR System Master Mix (#DBU-006; Bio-Genesis Technologies, Taipei, Taiwan) on a LightCycler 96 System (Roche, Indianapolis, IN, USA).

4.3. Western Blot Analysis

Whole-cell lysates were extracted by lysing cells in the radioimmunoprecipitation assay (RIPA) buffer containing 1× protease and phosphatase inhibitor cocktails. After separation by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), proteins were transferred to nitrocellulose membranes. Membranes were blocked with 5% skimmed milk in TBST buffer (20 mM Tris-base, 150 mM NaCl, and 0.05% Tween-20), and then blotted with a specific primary antibody and a corresponding horseradish peroxidase (HRP)-conjugated secondary antibody. Protein bands were developed with the Western Lightning Plus ECL detecting reagent (#NEL105001EA; PerkinElmer, Waltham, MA, USA) and detected using GE Amersham Imager 600 (GE Healthcare Life Sciences, Marlborough, MA, USA). E-cadherin (CDH1; #3195), N-cadherin (CDH2; #13116), and plakoglobin (JUP; #2309) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Claudin 4 (CLDN4; #GTX108483), collagen type I alpha 1 chain (COL1A1; #GTX112731), vimentin (VIM; #GTX100619), and GAPDH (#GTX100118) antibodies were purchased from GeneTex (Hsinchu, Taiwan). Uncropped images for each blot are shown in Figure S1.

4.4. Transfection

For the MIR31HG-knockdown analysis, small interfering (si)RNAs were transiently transfected into cells using the Lipofectamine RNAiMAX reagent (#13778150; ThermoFisher Scientific, Waltham, MA, USA). Lincode MIR31HG siRNA (SMARTpool; #R-187931-00-0005) and its non-targeting siRNA (#D-001320-10-05) were purchased from Horizon Discovery (Cambridge, UK). For the MIR31HG-overexpression analysis, pSL-MS2 (pMS2) and pSL-MS2-MIR31HG plasmids [14], kindly provided by Prof. Jing-Wen Shih (Taipei Medical University), were transfected into cells using the Lipofectamine 3000 reagent (#L3000015; ThermoFisher Scientific). Twenty-four hours after transfection, cells were used for further experiments.

4.5. Wound-Healing Assay

Cells (106) were grown in six-well plates for 24 h to reach a monolayer at more than 90% confluence. A wound in each well was created with a pipette tip. Floating cells were washed away with prewarmed phosphate-buffered saline (PBS), and then cells were treated with TGFβ in serum-free medium. Wounds were photographed in the same position at 0 and 48 h. Wound sizes were analyzed by an ImageJ plugin (Wound_healing_size_tool) [57]. The wound closure rate was calculated by the following formula: Wound closure (%) = [(A0h − A48h)/A0h] × 100%, where A0h is the initial wound size at time zero and A48h is the wound size after 48 h.

4.6. Statistical Analysis

Statistical analyses were performed using GraphPad Prism 9 (GraphPad Software, San Diego, CA, USA). Data were initially tested for normality using the Kolmogorov–Smirnov test. Parametric (normally distributed) data were analyzed using an unpaired two-tailed Student′s t-test. Nonparametric (non-normally distributed) data were analyzed with the two-tailed Mann–Whitney test. A p value of <0.05 was considered statistically significant.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/ijms23126559/s1.

Author Contributions

Conceptualization, C.-C.K. and P.-M.Y.; methodology, C.-C.K. and P.-M.Y.; validation, C.-C.K., Y.-Y.H. and P.-M.Y.; formal analysis, Y.-Y.H.; investigation, Y.-Y.H.; resources, P.-M.Y.; data curation, Y.-Y.H.; writing—original draft preparation, C.-C.K., Y.-Y.H. and P.-M.Y.; writing—review and editing, C.-C.K. and P.-M.Y.; visualization, Y.-Y.H.; supervision, C.-C.K. and P.-M.Y.; project administration, C.-C.K. and P.-M.Y.; funding acquisition, C.-C.K. and P.-M.Y. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Chi Mei Medical Center, grant number 110CM-TMU-14; the Ministry of Education, grant numbers DP2-108-21121-01-C-03-05 and DP2-111-21121-01-C-01-05; and the health and welfare surcharge of tobacco products (WanFang Hospital, Chi-Mei Medical Center, and Hualien Tzu-Chi Hospital Joint Cancer Center Grant-Focus on Colon Cancer Research), grant numbers MOHW110-TDU-B-212-144020 and MOHW111-TDU-B-221-014013.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data supporting this study can be obtained from the public databases or are available on request from the corresponding author.

Acknowledgments

We thank Hsin-Yi Chen (Taipei Medical University) for providing the PANC-1 cell line, and Jing-Wen Shih (Taipei Medical University) for providing the pSL-MS2 and pSL-MS2-MIR31HG plasmids. We also thank the financial support from the “TMU Research Center of Cancer Translational Medicine” from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan, as well as the Office of Research and Development at Taipei Medical University for the support in English editing.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. MIR31HG overexpression in pancreatic ductal adenocarcinoma (PDAC) patients: (A) MIR31HG expressions in pancreatic tumor and normal tissues were obtained from the GEPIA database, while 151 pancreatic tumors and four normal tissues were from the TCGA PanCancer Atlas dataset. Another 167 normal pancreatic tissues were from the GTEx database; (B) MIR31HG expressions in normal and pancreatic cancer tissues were obtained from two pancreatic cancer cohorts (GSE16515 and GSE28735) in the GEO database; (C) Kaplan-Meier overall and disease-free survival plots for cancer patients (TCGA PanCancer Atlas dataset) with high and low MIR31HG expression were generated using the GEPIA database; (D) Kaplan-Meier overall survival plots for cancer patients (GSE28735) with high and low MIR31HG expression were generated using PROGgeneV2 database.
Figure 1. MIR31HG overexpression in pancreatic ductal adenocarcinoma (PDAC) patients: (A) MIR31HG expressions in pancreatic tumor and normal tissues were obtained from the GEPIA database, while 151 pancreatic tumors and four normal tissues were from the TCGA PanCancer Atlas dataset. Another 167 normal pancreatic tissues were from the GTEx database; (B) MIR31HG expressions in normal and pancreatic cancer tissues were obtained from two pancreatic cancer cohorts (GSE16515 and GSE28735) in the GEO database; (C) Kaplan-Meier overall and disease-free survival plots for cancer patients (TCGA PanCancer Atlas dataset) with high and low MIR31HG expression were generated using the GEPIA database; (D) Kaplan-Meier overall survival plots for cancer patients (GSE28735) with high and low MIR31HG expression were generated using PROGgeneV2 database.
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Figure 2. Gene set enrichment analysis (GSEA) revealed the potential role of MIR31HG in transforming growth factor β (TGFβ) signaling and the epithelial-to-mesenchymal transition (EMT): (A) A GSEA was performed to enrich 50 cancer hallmarks in pancreatic tumor tissues; (B) A GSEA was performed to enrich the EMT gene signature in two pancreatic cancer cohorts (GSE16515 and GSE28735). FDR, false-discovery rate.
Figure 2. Gene set enrichment analysis (GSEA) revealed the potential role of MIR31HG in transforming growth factor β (TGFβ) signaling and the epithelial-to-mesenchymal transition (EMT): (A) A GSEA was performed to enrich 50 cancer hallmarks in pancreatic tumor tissues; (B) A GSEA was performed to enrich the EMT gene signature in two pancreatic cancer cohorts (GSE16515 and GSE28735). FDR, false-discovery rate.
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Figure 3. Transforming growth factor β (TGFβ) induced MIR31HG and the epithelial-to-mesenchymal transition (EMT) in PANC-1 cells: (A) A microarray dataset (GSE23952) for TGFβ (5 ng/mL for 48 h)-treated PANC-1 cells was obtained from the GEO database. A heat map shows the relative expressions of MIR31HG and EMT markers; (B) PANC-1 cells were treated with 5 ng/mL TGFβ for 24, 48, and 72 h. Total RNAs were examined by a real-time qPCR for MIR31HG and EMT marker expressions. Data represent the fold changes of mRNA expression (mean ± standard deviation, SD) for each gene compared to untreated control cells at each time point. * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate statistical significance compared to untreated control cells; (C) PANC-1 cells were treated with 5 ng/mL TGFβ for 24, 48, and 72 h. Total protein lysates were examined by Western blotting for EMT marker expressions. Representative images of each protein were obtained from the same or different blots. The band intensity was quantified and the related ratio to each time point was shown; (D) PANC-1 cells were treated with 5 ng/mL TGFβ for 72 h. The cell morphology was observed under bright-field microscopy. Scale bar, 50 μm.
Figure 3. Transforming growth factor β (TGFβ) induced MIR31HG and the epithelial-to-mesenchymal transition (EMT) in PANC-1 cells: (A) A microarray dataset (GSE23952) for TGFβ (5 ng/mL for 48 h)-treated PANC-1 cells was obtained from the GEO database. A heat map shows the relative expressions of MIR31HG and EMT markers; (B) PANC-1 cells were treated with 5 ng/mL TGFβ for 24, 48, and 72 h. Total RNAs were examined by a real-time qPCR for MIR31HG and EMT marker expressions. Data represent the fold changes of mRNA expression (mean ± standard deviation, SD) for each gene compared to untreated control cells at each time point. * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate statistical significance compared to untreated control cells; (C) PANC-1 cells were treated with 5 ng/mL TGFβ for 24, 48, and 72 h. Total protein lysates were examined by Western blotting for EMT marker expressions. Representative images of each protein were obtained from the same or different blots. The band intensity was quantified and the related ratio to each time point was shown; (D) PANC-1 cells were treated with 5 ng/mL TGFβ for 72 h. The cell morphology was observed under bright-field microscopy. Scale bar, 50 μm.
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Figure 4. Knockdown of MIR31HG reversed transforming growth factor β (TGFβ)-induced epithelial-to-mesenchymal transition (EMT) phenotypes: (A) PANC-1 cells were transfected with MIR31HG or control siRNA for 24 h and then exposed to 5 ng/mL TGFβ for another 48 h. The cell morphology was observed under bright-field microscopy. The arrows indicate cells with spindle-shaped, fibroblast-like morphology. Scale bar, 25 μm; (B) PANC-1 cells were transfected with MIR31HG or control siRNA for 24 h and then exposed to 5 ng/mL TGFβ for another 24 h. Total RNAs were examined by a real-time qPCR for MIR31HG and EMT marker expressions. Results are shown as the mean ± standard deviation (SD). * p < 0.05 and *** p < 0.001 or # p < 0.05, ## p < 0.01, and ### p < 0.001 indicate statistical significance between groups. ns, no statistical significance between indicated groups; (C) PANC-1 cells were transfected with MIR31HG or control siRNA for 24 h and then exposed to 5 ng/mL TGFβ for another 48 h. Total protein lysates were examined by Western blotting for EMT marker expressions. Representative images of each protein were obtained from the same or different blots. The band intensity was quantified and the related ratio to untreated si-NC cells was shown; (D) PANC-1 cells were transfected with a MIR31HG-encoding or control plasmid for 24 h and then exposed to 5 ng/mL TGFβ for another 24 h. Cell morphology was observed under bright-field microscopy. The arrows indicate cells with spindle-shaped, fibroblast-like morphology. Scale bar, 25 μm; (E) PANC-1 cells were transfected with a MIR31HG-encoding or control plasmid for 24 h and then exposed to 5 ng/mL TGFβ for another 24 h. Total RNAs were examined by a real-time qPCR for MIR31HG and EMT marker expressions. Results are shown as the mean ± standard deviation (SD). * p < 0.05, ** p < 0.01, and *** p < 0.001 or # p < 0.05, ## p < 0.01, and ### p < 0.001 indicate statistical significance between groups. ns, no statistical significance between indicated groups; (F) PANC-1 cells were transfected with a MIR31HG-encoding or control plasmid for 24 h and then exposed to 5 ng/mL TGFβ for another 48 h. Total protein lysates were examined by Western blotting for EMT marker expressions. Representative images of each protein were obtained from the same or different blots. The band intensity was quantified and the related ratio to untreated pMS2 cells was shown.
Figure 4. Knockdown of MIR31HG reversed transforming growth factor β (TGFβ)-induced epithelial-to-mesenchymal transition (EMT) phenotypes: (A) PANC-1 cells were transfected with MIR31HG or control siRNA for 24 h and then exposed to 5 ng/mL TGFβ for another 48 h. The cell morphology was observed under bright-field microscopy. The arrows indicate cells with spindle-shaped, fibroblast-like morphology. Scale bar, 25 μm; (B) PANC-1 cells were transfected with MIR31HG or control siRNA for 24 h and then exposed to 5 ng/mL TGFβ for another 24 h. Total RNAs were examined by a real-time qPCR for MIR31HG and EMT marker expressions. Results are shown as the mean ± standard deviation (SD). * p < 0.05 and *** p < 0.001 or # p < 0.05, ## p < 0.01, and ### p < 0.001 indicate statistical significance between groups. ns, no statistical significance between indicated groups; (C) PANC-1 cells were transfected with MIR31HG or control siRNA for 24 h and then exposed to 5 ng/mL TGFβ for another 48 h. Total protein lysates were examined by Western blotting for EMT marker expressions. Representative images of each protein were obtained from the same or different blots. The band intensity was quantified and the related ratio to untreated si-NC cells was shown; (D) PANC-1 cells were transfected with a MIR31HG-encoding or control plasmid for 24 h and then exposed to 5 ng/mL TGFβ for another 24 h. Cell morphology was observed under bright-field microscopy. The arrows indicate cells with spindle-shaped, fibroblast-like morphology. Scale bar, 25 μm; (E) PANC-1 cells were transfected with a MIR31HG-encoding or control plasmid for 24 h and then exposed to 5 ng/mL TGFβ for another 24 h. Total RNAs were examined by a real-time qPCR for MIR31HG and EMT marker expressions. Results are shown as the mean ± standard deviation (SD). * p < 0.05, ** p < 0.01, and *** p < 0.001 or # p < 0.05, ## p < 0.01, and ### p < 0.001 indicate statistical significance between groups. ns, no statistical significance between indicated groups; (F) PANC-1 cells were transfected with a MIR31HG-encoding or control plasmid for 24 h and then exposed to 5 ng/mL TGFβ for another 48 h. Total protein lysates were examined by Western blotting for EMT marker expressions. Representative images of each protein were obtained from the same or different blots. The band intensity was quantified and the related ratio to untreated pMS2 cells was shown.
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Figure 5. Knockdown of MIR31HG reversed transforming growth factor β (TGFβ)-induced cell migration: (A) PANC-1 cells were transfected with MIR31HG or control siRNA for 24 h and then a wound was created and photographed before (0 h) and after treatment with 5 ng/mL TGFβ for 48 h. Cell migration was examined by the wound closure rate. Scale bar, 50 μm; (B) Results in (A) were quantified (mean ± standard error of the mean, SEM). * p < 0.05 and ** p < 0.01 indicate statistical significance between groups.
Figure 5. Knockdown of MIR31HG reversed transforming growth factor β (TGFβ)-induced cell migration: (A) PANC-1 cells were transfected with MIR31HG or control siRNA for 24 h and then a wound was created and photographed before (0 h) and after treatment with 5 ng/mL TGFβ for 48 h. Cell migration was examined by the wound closure rate. Scale bar, 50 μm; (B) Results in (A) were quantified (mean ± standard error of the mean, SEM). * p < 0.05 and ** p < 0.01 indicate statistical significance between groups.
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Table
Table 1. Primer pairs used in this study.
Table 1. Primer pairs used in this study.
GeneSequenceProduct Length
MIR31HGForward: 5′-CACCAAGGTGTTCCTGCCTA-3′147 bp
Reverse: 5′-CAACCAGGCCAAAAGCATCC-3′
CDH1Forward: 5′-TACACTGCCCAGGAGCCAGA-3′103 bp
Reverse: 5′-TGGCACCAGTGTCCGGATTA-3′
CLDN4Forward: 5′-CGCATCAGGACTGGCTTTATCTC-3′187 bp
Reverse: 5′-CAGCGCGATGCCCATTA-3′
VIMForward: 5′-AGTCCACTGAGTACCGGAGAC-3′98 bp
Reverse: 5′-CATTTCACGCATCTGGCGTTC-3′
COL1A1Forward: 5′-CGGAGGAGAGTCAGGAAGG-3′153 bp
Reverse: 5′-ACATCAAGACAAGAACGAGGTAG-3′
SNAI1Forward: 5′-ACCACTATGCCGCGCTCTT-3′115 bp
Reverse: 5′-GGTCGTAGGGCTGCTGGAA-3′
SNAI2Forward: 5′-TGTTGCAGTGAGGGCAAGAA-3′72 bp
Reverse: 5′-GACCCTGGTTGCTTCAAGGA-3′
β-actinForward: 5′-GTTGCTATCCAGGCTGTGCT-3′113 bp
Reverse: 5′-AGGGCATACCCCTCGTAGAT-3′
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Ko, C.-C.; Hsieh, Y.-Y.; Yang, P.-M. Long Non-Coding RNA MIR31HG Promotes the Transforming Growth Factor β-Induced Epithelial-Mesenchymal Transition in Pancreatic Ductal Adenocarcinoma Cells. Int. J. Mol. Sci. 2022, 23, 6559. https://doi.org/10.3390/ijms23126559

AMA Style

Ko C-C, Hsieh Y-Y, Yang P-M. Long Non-Coding RNA MIR31HG Promotes the Transforming Growth Factor β-Induced Epithelial-Mesenchymal Transition in Pancreatic Ductal Adenocarcinoma Cells. International Journal of Molecular Sciences. 2022; 23(12):6559. https://doi.org/10.3390/ijms23126559

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Ko, Ching-Chung, Yao-Yu Hsieh, and Pei-Ming Yang. 2022. "Long Non-Coding RNA MIR31HG Promotes the Transforming Growth Factor β-Induced Epithelial-Mesenchymal Transition in Pancreatic Ductal Adenocarcinoma Cells" International Journal of Molecular Sciences 23, no. 12: 6559. https://doi.org/10.3390/ijms23126559

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