N-Methyltransferase CaASHH3 Acts as a Positive Regulator of Immunity against Bacterial Pathogens in Pepper
Abstract
:1. Introduction
2. Results
2.1. Cloning and Sequence Analysis of the Full-Length cDNA of CaASHH3
2.2. Transcriptional Expression Levels of CaASHH3 Were Upregulated by R. solanacearum Inoculation and Exogenously Supplied Phytohormones including SA, MeJA, ETH, and ABA
2.3. CaASHH3 Was Localized to the Nucleus
2.4. CaASHH3-Silencing Compromised the Resistance of Pepper against R. solanacearum Infection
2.5. Transient Over-Expression of CaASHH3 Caused HR-like Cell Death and Accumulation of H2O2 in the Leaves of Pepper Plants
2.6. ChIP-PCR Analysis to Test Chromatin Modification in Pepper
2.7. CaASHH3-OX (Over-Expression) in Transgenic Arabidopsis Plants Enhanced the Resistance to Bacterial Pathogen
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Vectors Construction
4.3. Pathogens and Inoculation Procedures
4.4. Treatment of Pepper Plants with Exogenously Applied Phytohormones
4.5. Subcellular Localization Experiment of CaASHH3
4.6. Virus-Induced Gene Silencing (VIGS) in Pepper Plants
4.7. Agrobacterium-Mediated Transient CaASHH3 Over-Expression Assay
4.8. Histochemical Staining
4.9. RNA Extraction and Real-Time qRT-PCR
4.10. Measurement of Ion Conductivity
4.11. Immunoblotting
4.12. ChIP Assays
4.13. Generation of CaASHH3-OX-Overexpressing Transgenic Arabidopsis Plants
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hussain, A.; Kaisheng, L.; Noman, A.; Ashraf, M.F.; Albaqami, M.; Khan, M.I.; Liu, Z.; He, S. N-Methyltransferase CaASHH3 Acts as a Positive Regulator of Immunity against Bacterial Pathogens in Pepper. Int. J. Mol. Sci. 2022, 23, 6492. https://doi.org/10.3390/ijms23126492
Hussain A, Kaisheng L, Noman A, Ashraf MF, Albaqami M, Khan MI, Liu Z, He S. N-Methyltransferase CaASHH3 Acts as a Positive Regulator of Immunity against Bacterial Pathogens in Pepper. International Journal of Molecular Sciences. 2022; 23(12):6492. https://doi.org/10.3390/ijms23126492
Chicago/Turabian StyleHussain, Ansar, Liu Kaisheng, Ali Noman, Muhammad Furqan Ashraf, Mohammed Albaqami, Muhammad Ifnan Khan, Zhiqin Liu, and Shuilin He. 2022. "N-Methyltransferase CaASHH3 Acts as a Positive Regulator of Immunity against Bacterial Pathogens in Pepper" International Journal of Molecular Sciences 23, no. 12: 6492. https://doi.org/10.3390/ijms23126492