Picrasidine I Triggers Heme Oxygenase-1-Induced Apoptosis in Nasopharyngeal Carcinoma Cells via ERK and Akt Signaling Pathways
Abstract
:1. Introduction
2. Results
2.1. Picrasidine I Decreased the Cell Viability of NPC Cell Lines and Caused Cell Accumulation in Sub-G1, S, and G2/M Phases
2.2. Picrasidine I Induced Apoptosis in NPC Cells via Extrinsic and Intrinsic Pathways
2.3. The Activation of Mitogen-Activated Protein Kinases (MAPKs) and Akt Signaling Pathways Is Involved in Picrasidine I-Induced Apoptosis
2.4. HO-1 Participates in Picrasidine I-Induced Apoptosis via Activating the ERK1/2 and Akt Signaling Pathways
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Culture Conditions
4.2. Cell Viability Assay
4.3. Colony Formation Assay
4.4. Cell Cycle Analysis
4.5. Western Blot Analysis
4.6. Nuclear Morphology Analysis
4.7. Apoptosis Analysis
4.8. Mitochondrial Membrane Potential Analysis
4.9. Caspase-3/7 Activity Assay
4.10. Proteome Profiler Analysis
4.11. RNA Interference Experiments
4.12. Bioinformatic Analysis
4.13. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ho, H.-Y.; Chen, P.-J.; Chuang, Y.-C.; Lo, Y.-S.; Lin, C.-C.; Hsieh, M.-J.; Chen, M.-K. Picrasidine I Triggers Heme Oxygenase-1-Induced Apoptosis in Nasopharyngeal Carcinoma Cells via ERK and Akt Signaling Pathways. Int. J. Mol. Sci. 2022, 23, 6103. https://doi.org/10.3390/ijms23116103
Ho H-Y, Chen P-J, Chuang Y-C, Lo Y-S, Lin C-C, Hsieh M-J, Chen M-K. Picrasidine I Triggers Heme Oxygenase-1-Induced Apoptosis in Nasopharyngeal Carcinoma Cells via ERK and Akt Signaling Pathways. International Journal of Molecular Sciences. 2022; 23(11):6103. https://doi.org/10.3390/ijms23116103
Chicago/Turabian StyleHo, Hsin-Yu, Ping-Ju Chen, Yi-Ching Chuang, Yu-Sheng Lo, Chia-Chieh Lin, Ming-Ju Hsieh, and Mu-Kuan Chen. 2022. "Picrasidine I Triggers Heme Oxygenase-1-Induced Apoptosis in Nasopharyngeal Carcinoma Cells via ERK and Akt Signaling Pathways" International Journal of Molecular Sciences 23, no. 11: 6103. https://doi.org/10.3390/ijms23116103