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Article

A Micro-Immunotherapy Sequential Medicine MIM-seq Displays Immunomodulatory Effects on Human Macrophages and Anti-Tumor Properties towards In Vitro 2D and 3D Models of Colon Carcinoma and in an In Vivo Subcutaneous Xenograft Colon Carcinoma Model

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Preclinical Research Department, Labo’Life France, 1 rue François Bruneau, 44000 Nantes, France
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Kitos Biotech s.r.l.s., Porto Conte Ricerche, S.P. 55 Porto Conte—Capo Caccia, Km 8,400 Loc. Tramariglio, 07041 Alghero, Italy
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ProfileHIT, 7 rue du Buisson, 44680 Sainte-Pazanne, France
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Centre d’Investigation Clinique Hématologie CHU Hôtel Dieu, 44093 Nantes, France
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VivaCell Biotechnology GmbH, 79211 Denzlingen, Germany
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InFlectis Bioscience, 44200 Nantes, France
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Author to whom correspondence should be addressed.
Academic Editor: Antonio Maccio
Int. J. Mol. Sci. 2022, 23(11), 6059; https://doi.org/10.3390/ijms23116059
Received: 29 April 2022 / Revised: 20 May 2022 / Accepted: 25 May 2022 / Published: 27 May 2022
(This article belongs to the Special Issue Molecular Basis and Advances of Targeted Immunotherapy for Cancer)
In this study, the immunomodulatory effects of a sequential micro-immunotherapy medicine, referred as MIM-seq, were appraised in human primary M1 and M2 macrophages, in which the secretion of pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, IL-12, IL-23, and tumor necrosis factor (TNF)-alpha, was inhibited. In addition, the potential anti-proliferative effects of MIM-seq on tumor cells was assessed in three models of colorectal cancer (CRC): an in vitro two-dimensions (2D) model of HCT-116 cells, an in vitro tri-dimensional (3D) model of spheroids, and an in vivo model of subcutaneous xenografted mice. In these models, MIM-seq displayed anti-proliferative effects when compared with the vehicle. In vivo, the tumor growth was slightly reduced in MIM-seq-treated animals. Moreover, MIM-seq could slightly reduce the growth of our spheroid models, especially under serum-deprivation. When MIM-seq was combined with two well-known anti-cancerogenic agents, either resveratrol or etoposide, MIM-seq could even further reduce the spheroid’s volume, pointing up the need to further assess whether MIM-seq could be beneficial for CRC patients as an adjuvant therapy. Altogether, these data suggest that MIM-seq could have anti-tumor properties against CRC and an immunomodulatory effect towards the mediators of inflammation, whose systemic dysregulation is considered to be a poor prognosis for patients. View Full-Text
Keywords: micro-immunotherapy; MIM-seq; macrophages; immunomodulation; potential anti-tumor agent; colorectal cancer; 3D tumor spheroids micro-immunotherapy; MIM-seq; macrophages; immunomodulation; potential anti-tumor agent; colorectal cancer; 3D tumor spheroids
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MDPI and ACS Style

Jacques, C.; Marchesi, I.; Fiorentino, F.P.; Chatelais, M.; Lilli, N.L.; Appel, K.; Lejeune, B.; Floris, I. A Micro-Immunotherapy Sequential Medicine MIM-seq Displays Immunomodulatory Effects on Human Macrophages and Anti-Tumor Properties towards In Vitro 2D and 3D Models of Colon Carcinoma and in an In Vivo Subcutaneous Xenograft Colon Carcinoma Model. Int. J. Mol. Sci. 2022, 23, 6059. https://doi.org/10.3390/ijms23116059

AMA Style

Jacques C, Marchesi I, Fiorentino FP, Chatelais M, Lilli NL, Appel K, Lejeune B, Floris I. A Micro-Immunotherapy Sequential Medicine MIM-seq Displays Immunomodulatory Effects on Human Macrophages and Anti-Tumor Properties towards In Vitro 2D and 3D Models of Colon Carcinoma and in an In Vivo Subcutaneous Xenograft Colon Carcinoma Model. International Journal of Molecular Sciences. 2022; 23(11):6059. https://doi.org/10.3390/ijms23116059

Chicago/Turabian Style

Jacques, Camille, Irene Marchesi, Francesco Paolo Fiorentino, Mathias Chatelais, Nicoletta Libera Lilli, Kurt Appel, Beatrice Lejeune, and Ilaria Floris. 2022. "A Micro-Immunotherapy Sequential Medicine MIM-seq Displays Immunomodulatory Effects on Human Macrophages and Anti-Tumor Properties towards In Vitro 2D and 3D Models of Colon Carcinoma and in an In Vivo Subcutaneous Xenograft Colon Carcinoma Model" International Journal of Molecular Sciences 23, no. 11: 6059. https://doi.org/10.3390/ijms23116059

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