Dietary Interventions Ameliorate Infectious Colitis by Restoring the Microbiome and Promoting Stem Cell Proliferation in Mice

Ahmed, Ishfaq; Yusuf, Kafayat; Roy, Badal C.; Stubbs, Jason; Anant, Shrikant; Attard, Thomas M.; Sampath, Venkatesh; Umar, Shahid

doi:10.3390/ijms23010339

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Dietary Interventions Ameliorate Infectious Colitis by Restoring the Microbiome and Promoting Stem Cell Proliferation in Mice

^1,†,

^1,†

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^1,*

Department of Surgery, University of Kansas Medical Center, Kansas City, KS 66160, USA

Department of Internal Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA

Cancer Biology Department, University of Kansas Medical Center, Kansas City, KS 66160, USA

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Department of Pediatrics and Gastroenterology, Children’s Mercy Hospital, Kansas City, KS 66160, USA

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Academic Editor: Angelo Viscido

Int. J. Mol. Sci. 2022, 23(1), 339; https://doi.org/10.3390/ijms23010339

Received: 9 November 2021 / Revised: 21 December 2021 / Accepted: 25 December 2021 / Published: 29 December 2021

(This article belongs to the Special Issue Precision Medicine in Inflammatory Bowel Diseases: Epigenetics, Gut Microbiome, Sex Differences and Beyond)

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Abstract

Decreases in short-chain-fatty-acids (SCFAs) are linked to inflammatory bowel disease (IBD). Yet, the mechanisms through which SCFAs promote wound healing, orchestrated by intestinal stem cells, are poorly understood. We discovered that, in mice with Citrobacter rodentium (CR)-induced infectious colitis, treatment with Pectin and Tributyrin diets reduced the severity of colitis by restoring Firmicutes and Bacteroidetes and by increasing mucus production. RNA-seq in young adult mouse colon (YAMC) cells identified higher expression of Lgr4, Lgr6, DCLK1, Muc2, and SIGGIR after Butyrate treatment. Lineage tracing in CR-infected Lgr5-EGFP-IRES-CreERT2/ROSA26-LacZ (Lgr5-R) mice also revealed an expansion of LacZ-labeled Lgr5(+) stem cells in the colons of both Pectin and Tributyrin-treated mice compared to control. Interestingly, gut microbiota was required for Pectin but not Tributyrin-induced Lgr5(+) stem cell expansion. YAMC cells treated with sodium butyrate exhibited increased Lgr5 promoter reporter activity due to direct Butyrate binding with Lgr5 at −4.0 Kcal/mol, leading to thermal stabilization. Upon ChIP-seq, H3K4me3 increased near Lgr5 transcription start site that contained the consensus binding motif for a transcriptional activator of Lgr5 (SPIB). Thus, a multitude of effects on gut microbiome, differential gene expression, and/or expansion of Lgr5(+) stem cells seem to underlie amelioration of colitis following dietary intervention. View Full-Text

Keywords: intestinal stem cells; lgr5; gut microbiome; Citrobacter rodentium; colitis; Pectin; Tributyrin; short-chain-fatty-acids (SCFAs); bacterial infection; dietary intervention

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Ahmed, I.; Yusuf, K.; Roy, B.C.; Stubbs, J.; Anant, S.; Attard, T.M.; Sampath, V.; Umar, S. Dietary Interventions Ameliorate Infectious Colitis by Restoring the Microbiome and Promoting Stem Cell Proliferation in Mice. Int. J. Mol. Sci. 2022, 23, 339. https://doi.org/10.3390/ijms23010339

AMA Style

Ahmed I, Yusuf K, Roy BC, Stubbs J, Anant S, Attard TM, Sampath V, Umar S. Dietary Interventions Ameliorate Infectious Colitis by Restoring the Microbiome and Promoting Stem Cell Proliferation in Mice. International Journal of Molecular Sciences. 2022; 23(1):339. https://doi.org/10.3390/ijms23010339

Chicago/Turabian Style

Ahmed, Ishfaq, Kafayat Yusuf, Badal C. Roy, Jason Stubbs, Shrikant Anant, Thomas M. Attard, Venkatesh Sampath, and Shahid Umar. 2022. "Dietary Interventions Ameliorate Infectious Colitis by Restoring the Microbiome and Promoting Stem Cell Proliferation in Mice" International Journal of Molecular Sciences 23, no. 1: 339. https://doi.org/10.3390/ijms23010339

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Dietary Interventions Ameliorate Infectious Colitis by Restoring the Microbiome and Promoting Stem Cell Proliferation in Mice

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