Dietary Interventions Ameliorate Infectious Colitis by Restoring the Microbiome and Promoting Stem Cell Proliferation in Mice
Abstract
:1. Introduction
2. Results
2.1. Pectin and Tributyrin Diets Ameliorate Symptoms of Colitis
2.2. Pectin and Tributyrin Diets Enhanced MUC2 Secretion in CR-Infected Groups
2.3. Microbial Diversity and Composition Changes Following Pectin Diet
2.4. Pectin Fermentation Product Butyrate Induces Differential Expression of Genes Involved in Epithelial Regeneration and Repair
2.5. Pectin and Tributyrin Diets Regulate Lgr5 Expression
2.6. Butyrate Increases LGR5 Promoter Activity
2.7. Butyrate Promotes Active Transcription of Lgr5 through SPIB
3. Discussion
4. Methods
4.1. Animals
4.2. Lineage Tracing
4.3. Treatments and Humane Endpoints
4.4. Histology and Electron Microscopy
4.5. Immunohistochemistry
4.6. Immunofluorescence
4.7. Fluorescence In Situ Hybridization (FISH)
4.8. Bacterial DNA Extraction
4.9. Microbial Analysis Using 16S Ribosomal DNA Library Preparation and Sequencing
4.10. Functional Profiling of the Microbial Community
4.11. Fluorescein Isothiocyanate-Dextran (FITC-D) Assay
4.12. ChIP-Sequencing and Data Analysis
4.13. Lgr5 Promoter Activity Reporter Assay
4.14. Cellular Thermal Shift Assay (CETSA) and Western Blotting
4.15. Molecular Docking
4.16. RNA Sequencing
4.17. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genes | Fold Change CR Infection vs. Control | Fold Change Butyrate Treatment vs. Control | Fold Change Butyrate Treatment vs. CR Infection |
---|---|---|---|
DCLK 1 | −2.5 | −1.1 | 2.3 |
DLL1 | −1.8 | 1.5 | 2.7 |
FZD9 | 2.2 | 6.1 | 2.8 |
HES 1 | −1.2 | 1.2 | 1.4 |
IL 18 | −2.1 | −1.3 | 1.6 |
IL 33 | −1.7 | 1.8 | 3.1 |
IL 4 | 1.1 | 2.3 | 2.1 |
IL 6 | 3.2 | 2.1 | −1.5 |
JAG 1 | −1.1 | 4.6 | 5.1 |
LEF 1 | 1.4 | 10.4 | 7.5 |
LGR 4 | −1 | 2.2 | 2.2 |
LGR 6 | 1.6 | 9.1 | 5.7 |
LRP 5 | −1 | 1.5 | 1.5 |
LRP 6 | −1.1 | 1.5 | 1.7 |
MUC 2 | −1 | 8.7 | 9.1 |
MYC | 1.7 | 2.3 | 1.4 |
NOD 2 | 1.5 | 12.2 | 7.9 |
NOTCH 3 | −1.2 | 10.6 | 12.3 |
NOTCH 4 | −1.6 | 4.5 | 7.4 |
RSP0 1 | −3.4 | −1.3 | 2.6 |
RSP0 3 | −1.2 | 2.8 | 3.4 |
SIGIRR | 1.8 | 28.9 | 16.3 |
TGF-Alpha | 2.1 | 21.1 | 10.2 |
TGF-Beta | −1.7 | 10 | 17 |
VILL | −1.9 | 10.6 | 20.5 |
WNT 2B | −3.1 | −1.3 | 2.3 |
WNT 7A | 1.4 | 52.6 | 36.3 |
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Ahmed, I.; Yusuf, K.; Roy, B.C.; Stubbs, J.; Anant, S.; Attard, T.M.; Sampath, V.; Umar, S. Dietary Interventions Ameliorate Infectious Colitis by Restoring the Microbiome and Promoting Stem Cell Proliferation in Mice. Int. J. Mol. Sci. 2022, 23, 339. https://doi.org/10.3390/ijms23010339
Ahmed I, Yusuf K, Roy BC, Stubbs J, Anant S, Attard TM, Sampath V, Umar S. Dietary Interventions Ameliorate Infectious Colitis by Restoring the Microbiome and Promoting Stem Cell Proliferation in Mice. International Journal of Molecular Sciences. 2022; 23(1):339. https://doi.org/10.3390/ijms23010339
Chicago/Turabian StyleAhmed, Ishfaq, Kafayat Yusuf, Badal C. Roy, Jason Stubbs, Shrikant Anant, Thomas M. Attard, Venkatesh Sampath, and Shahid Umar. 2022. "Dietary Interventions Ameliorate Infectious Colitis by Restoring the Microbiome and Promoting Stem Cell Proliferation in Mice" International Journal of Molecular Sciences 23, no. 1: 339. https://doi.org/10.3390/ijms23010339