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Article

Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem Cells

Laboratory of Molecular Biology, Research Centre for Medical Genetics, 1 Moskvorechye St., 115522 Moscow, Russia
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Academic Editors: Dmitry Klokov and Andreyan N. Osipov
Int. J. Mol. Sci. 2022, 23(1), 261; https://doi.org/10.3390/ijms23010261
Received: 29 November 2021 / Revised: 22 December 2021 / Accepted: 24 December 2021 / Published: 27 December 2021
The concept of hormesis describes a phenomenon of adaptive response to low-dose ionizing radiation (LDIR). Similarly, the concept of mitohormesis states that the adaptive program in mitochondria is activated in response to minor stress effects. The mechanisms of hormesis effects are not clear, but it is assumed that they can be mediated by reactive oxygen species. Here, we studied effects of LDIR on mitochondria in mesenchymal stem cells. We have found that X-ray radiation at a dose of 10 cGy as well as oxidized fragments of cell-free DNA (cfDNA) at a concentration of 50 ng/mL resulted in an increased expression of a large number of genes regulating the function of the mitochondrial respiratory chain complexes in human mesenchymal stem cells (MSC). Several genes remained upregulated within hours after the exposure. Both X-ray radiation and oxidized cfDNA resulted in upregulation of FIS1 and MFN1 genes, which regulated fusion and fission of mitochondria, within 3–24 h after the exposure. Three hours after the exposure, the number of copies of mitochondrial DNA in cells had increased. These findings support the hypothesis that assumes oxidized cell-free DNA as a mediator of MSC response to low doses of radiation. View Full-Text
Keywords: low-dose ionizing radiation; mitohormesis; cell-free DNA; human mesenchymal stem cells; mitochondria low-dose ionizing radiation; mitohormesis; cell-free DNA; human mesenchymal stem cells; mitochondria
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MDPI and ACS Style

Kostyuk, S.V.; Proskurnina, E.V.; Konkova, M.S.; Abramova, M.S.; Kalianov, A.A.; Ershova, E.S.; Izhevskaya, V.L.; Kutsev, S.I.; Veiko, N.N. Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem Cells. Int. J. Mol. Sci. 2022, 23, 261. https://doi.org/10.3390/ijms23010261

AMA Style

Kostyuk SV, Proskurnina EV, Konkova MS, Abramova MS, Kalianov AA, Ershova ES, Izhevskaya VL, Kutsev SI, Veiko NN. Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem Cells. International Journal of Molecular Sciences. 2022; 23(1):261. https://doi.org/10.3390/ijms23010261

Chicago/Turabian Style

Kostyuk, Svetlana V., Elena V. Proskurnina, Marina S. Konkova, Margarita S. Abramova, Andrey A. Kalianov, Elizaveta S. Ershova, Vera L. Izhevskaya, Sergey I. Kutsev, and Natalia N. Veiko. 2022. "Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem Cells" International Journal of Molecular Sciences 23, no. 1: 261. https://doi.org/10.3390/ijms23010261

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