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Article

Cord Blood T Cells Expressing High and Low PKCζ Levels Develop into Cells with a Propensity to Display Th1 and Th9 Cytokine Profiles, Respectively

1
Department of Immunopathology, SA Pathology at the Women’s and Children’s Hospital, North Adelaide, SA 5006, Australia
2
Adelaide School of Medicine and the Robinson Research Institute, University of Adelaide, Adelaide, SA 5005, Australia
3
Department of Neonatal Medicine, Women’s and Children’s Hospital, North Adelaide, SA 5006, Australia
4
School of Paediatrics and Child Health, The University of Western Australia, 35 Stirling Highway, Perth, WA 6009, Australia
5
The ORIGINS Project, Telethon Kids Institute and Perth Children’s Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia
6
School of Biological Sciences, University of Adelaide, Adelaide, SA 5005, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Michael Roth
Int. J. Mol. Sci. 2021, 22(9), 4907; https://doi.org/10.3390/ijms22094907
Received: 30 March 2021 / Revised: 26 April 2021 / Accepted: 29 April 2021 / Published: 5 May 2021
(This article belongs to the Special Issue Molecular Mechanisms of Allergy and Asthma)
Low Protein Kinase C zeta (PKCζ) levels in cord blood T cells (CBTC) have been shown to correlate with the development of allergic sensitization in childhood. However, little is known about the mechanisms responsible. We have examined the relationship between the expression of different levels of PKCζ in CBTC and their development into mature T cell cytokine producers that relate to allergy or anti-allergy promoting cells. Maturation of naïve CBTC was initiated with anti-CD3/-CD28 antibodies and recombinant human interleukin-2 (rhIL-2). To stimulate lymphocyte proliferation and cytokine production the cells were treated with Phytohaemagglutinin (PHA) and Phorbol myristate acetate (PMA). Irrespective of the PKCζ levels expressed, immature CBTC showed no difference in lymphocyte proliferation and the production of T helper 2 (Th2) cytokine interleukin-4 (IL-4) and Th1 cytokine, interferon-gamma (IFN-γ), and influenced neither their maturation from CD45RA+ to CD45RO+ cells nor cell viability/apoptosis. However, upon maturation the low PKCζ expressing cells produced low levels of the Th1 cytokines, IFN-γ, IL-2 and tumour necrosis factor-alpha (TNF), no changes to levels of the Th2 cytokines, IL-4, IL-5 and IL-13, and an increase in the Th9 cytokine, IL-9. Other cytokines, lymphotoxin-α (LT-α), IL-10, IL-17, IL-21, IL-22 and Transforming growth factor-beta (TGF-β) were not significantly different. The findings support the view that low CBTC PKCζ levels relate to the increased risk of developing allergic diseases. View Full-Text
Keywords: cord blood T cells; T cell maturation; Th1 and Th2 subsets; PKCζ; cytokines; allergy cord blood T cells; T cell maturation; Th1 and Th2 subsets; PKCζ; cytokines; allergy
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MDPI and ACS Style

Perveen, K.; Quach, A.; McPhee, A.; Prescott, S.L.; Barry, S.C.; Hii, C.S.; Ferrante, A. Cord Blood T Cells Expressing High and Low PKCζ Levels Develop into Cells with a Propensity to Display Th1 and Th9 Cytokine Profiles, Respectively. Int. J. Mol. Sci. 2021, 22, 4907. https://doi.org/10.3390/ijms22094907

AMA Style

Perveen K, Quach A, McPhee A, Prescott SL, Barry SC, Hii CS, Ferrante A. Cord Blood T Cells Expressing High and Low PKCζ Levels Develop into Cells with a Propensity to Display Th1 and Th9 Cytokine Profiles, Respectively. International Journal of Molecular Sciences. 2021; 22(9):4907. https://doi.org/10.3390/ijms22094907

Chicago/Turabian Style

Perveen, Khalida, Alex Quach, Andrew McPhee, Susan L. Prescott, Simon C. Barry, Charles S. Hii, and Antonio Ferrante. 2021. "Cord Blood T Cells Expressing High and Low PKCζ Levels Develop into Cells with a Propensity to Display Th1 and Th9 Cytokine Profiles, Respectively" International Journal of Molecular Sciences 22, no. 9: 4907. https://doi.org/10.3390/ijms22094907

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