Cytoskeleton Rearrangements Modulate TRPC6 Channel Activity in Podocytes
Abstract
1. Introduction
2. Results
2.1. Disruption of the Actin Cytoskeleton with Cytochalasin D Affects Filopodial Extension and Cell Migration
2.2. Acute Disruption of Actin Microfilaments with Cytochalasin D Markedly Increases TRPC6 Activity (NPo) in CHO Cells and Rat Podocytes
2.3. ACTN4 K255E Reduces TRPC6 Activity (NPo) and Conductance
3. Discussion
4. Materials and Methods
4.1. Cells
4.2. Animals
4.3. Electrophysiological Analysis
4.4. Imaging of the Cytoskeleton
4.5. Scanning Ion Conductance Microscopy (SICM) Analysis
4.6. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Shalygin, A.; Shuyskiy, L.S.; Bohovyk, R.; Palygin, O.; Staruschenko, A.; Kaznacheyeva, E. Cytoskeleton Rearrangements Modulate TRPC6 Channel Activity in Podocytes. Int. J. Mol. Sci. 2021, 22, 4396. https://doi.org/10.3390/ijms22094396
Shalygin A, Shuyskiy LS, Bohovyk R, Palygin O, Staruschenko A, Kaznacheyeva E. Cytoskeleton Rearrangements Modulate TRPC6 Channel Activity in Podocytes. International Journal of Molecular Sciences. 2021; 22(9):4396. https://doi.org/10.3390/ijms22094396
Chicago/Turabian StyleShalygin, Alexey, Leonid S. Shuyskiy, Ruslan Bohovyk, Oleg Palygin, Alexander Staruschenko, and Elena Kaznacheyeva. 2021. "Cytoskeleton Rearrangements Modulate TRPC6 Channel Activity in Podocytes" International Journal of Molecular Sciences 22, no. 9: 4396. https://doi.org/10.3390/ijms22094396
APA StyleShalygin, A., Shuyskiy, L. S., Bohovyk, R., Palygin, O., Staruschenko, A., & Kaznacheyeva, E. (2021). Cytoskeleton Rearrangements Modulate TRPC6 Channel Activity in Podocytes. International Journal of Molecular Sciences, 22(9), 4396. https://doi.org/10.3390/ijms22094396